Microbial oil and extraction method thereof

A microbial oil and extraction method technology, applied in the direction of fat production, fat oil/fat production, etc., can solve the problems of low ARA and low extraction efficiency

Active Publication Date: 2019-01-11
梁云
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction efficiency of microbial oil by wet process is low at present, and the content of exotoxin in the obtained microbial oil is high, while the content of DHA (docosahexaenoic acid) and ARA (arachidonic acid) is low

Method used

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  • Microbial oil and extraction method thereof
  • Microbial oil and extraction method thereof
  • Microbial oil and extraction method thereof

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Experimental program
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Embodiment approach

[0023] According to a preferred embodiment of the present invention, the cell wall lyase includes alkaline protease. In addition, preferably, the cell wall lyase may also include at least one of cellulase, hemicellulase, pectinase, helicase, chitinase and ligninase.

[0024] According to the present invention, the dosage of the cell wall lyase can be selected within a wide range, as long as the cell wall of the oleaginous microorganism can be fully lysed, so as to release the microbial oil. Preferably, the amount of the cell wall lyase is 1-5 g per liter of the fermentation broth.

[0025] According to the present invention, the conditions of the enzymatic hydrolysis can be the conditions conventionally used to crack the cell wall of oleaginous microorganisms by enzymatic hydrolysis, but the inventors of the present invention have found that by performing the enzymatic hydrolysis under aerobic conditions, it is possible to The enzymatic hydrolysis efficiency is further improv...

Embodiment 1

[0057] This embodiment is used to illustrate microbial grease provided by the present invention and preparation method thereof

[0058] (1) lye preparation: add 200L of demineralized water in the alkali tank, take food-grade NaOH according to the concentration of 20% by weight and put it into the alkali tank to fully dissolve it, and place the prepared lye in a pressure of 0.14MPa. Sterilize in saturated steam at 145°C for 1 hour, then pass sterile compressed air into the alkali tank to make the tank pressure of the alkali tank 0.02-0.05MPa, and pass cooling water into the jacket of the alkali tank to cool the alkali solution to 35-45℃, standby;

[0059] (2) Preparation of cell wall lysate: sterilize the series-connected secondary liquid sterile filtration system with a pressure of 0.14 MPa and a temperature of 145° C. with saturated steam for 1 h, and then use sterile compressed air to keep the pressure for standby; the sterile filtration The filter specification of the syst...

Embodiment 2

[0063] This embodiment is used to illustrate microbial grease provided by the present invention and preparation method thereof

[0064] (1) lye preparation: add the demineralized water of 200L in the alkali tank, take food-grade sodium carbonate according to the concentration of 15% by weight and drop into the alkali tank to fully dissolve, the prepared lye is placed in a pressure of 0.14MPa, Sterilize in saturated steam at 145°C for 1 hour, then pass sterile compressed air into the alkali tank to make the tank pressure of the alkali tank 0.02-0.05MPa, and pass cooling water into the jacket of the alkali tank to cool the alkali solution to 35-45°C, set aside;

[0065] (2) Preparation of cell wall lysate: sterilize the series-connected secondary liquid sterile filtration system with a pressure of 0.14 MPa and a temperature of 145° C. with saturated steam for 1 h, and then use sterile compressed air to keep the pressure for standby; the sterile filtration The filter specificati...

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Abstract

The invention discloses a microbial oil and an extraction method thereof, belonging to the field of extraction of microbial oil. The extraction method comprises the following steps: (1) contacting a fermentation broth of an oil producing microorganism with a cell wall lyase in an aseptic environment to perform enzymolysis on the oil producing microorganism in the fermentation broth so as to obtaina mixed solution containing the microbial oil; and (2) subjecting the mixed solution containing the microbial oil obtained in the step (1) to oil-water-solid three-phase separation so as to obtain the microbial oil. With such a technical scheme, the whole process is controlled to be implemented in the aseptic environment, contaminative microbes are prevented in the extraction process of the microbial oil, foreign bacteria and toxins are prevented from migrating into the product, the safety of the microbial oil can be increased, and the yield of the obtained microbial oil used as crude oil canbe increased to 97-98%, which is significantly improved compared with the same industry; in addition, the DHA content or ARA content of the crude oil is significantly improved, and the content of extracellular toxins is significantly reduced.

Description

technical field [0001] The invention relates to the field of extracting microbial oils, in particular to a method for extracting microbial oils and microbial oils extracted by the method. Background technique [0002] Microbial oil is produced by biosynthesis and transformation of nitrogen sources, carbon sources, trace elements, etc. in the culture medium by oil-producing microorganisms such as fungi and bacteria. The oils produced are all wrapped by cell walls, and microbial oils are usually called intracellular oils. In order to obtain this type of intracellular oil, the cell walls of these microorganisms must be cracked, so the degree of cell wall cracking has become a key factor in the efficiency of oil extraction. [0003] At present, microbial oil extraction mainly has two extraction processes: wet method and dry method. The dry extraction process has problems such as high temperature dehydration resulting in large oil oxidation loss, the need to use organic solvent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/02C11B1/00
CPCC11B1/00C11B1/025
Inventor 瞿瀚鹏曹晟王身健
Owner 梁云
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