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Tryptophanol and xanthanin in urine as diagnostic markers for idiopathic male infertility and their application

A male infertility, idiopathic technology, applied in the field of analytical chemistry and clinical medicine, can solve problems that have not received corresponding attention, and achieve the effect of preventing disease progression, strong correlation, and easy detection

Active Publication Date: 2022-04-08
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of metabolomics analysis of urinary metabolites in the diagnostic monitoring of idiopathic male infertility has not received corresponding attention

Method used

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  • Tryptophanol and xanthanin in urine as diagnostic markers for idiopathic male infertility and their application
  • Tryptophanol and xanthanin in urine as diagnostic markers for idiopathic male infertility and their application
  • Tryptophanol and xanthanin in urine as diagnostic markers for idiopathic male infertility and their application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Example 1: Research object selection and grouping basis

[0106] The subjects of this part of the study were first-diagnosed cases of idiopathic male infertility and healthy reproductive controls from the Affiliated Hospital of Nanjing Medical University. The research content and informed consent form were approved by the Ethics Committee of Nanjing Medical University and met the requirements of relevant regulations. Cases and controls signed the informed consent after learning the content. All study subjects underwent a complete physical examination and completed a questionnaire that included basic personal information, living habits, occupational and environmental exposures, genetic risk factors, sexual and reproductive function, disease history, and physical activity. The first phase included 607 eligible cases of idiopathic male infertility and 430 healthy controls; the second phase included 15 eligible cases of idiopathic male infertility and 15 healthy controls a...

Embodiment 2

[0137] Example 2: UPLC-MS Metabolomics Biomarker Screening for Idiopathic Male Infertility

[0138] 1. Sample pretreatment

[0139] 1.1. Take 300 μL of urine, add 10 μL of internal standard A, add 10 μL of internal standard B, add 10 μL of internal standard C, add 40 μL of methanol (reagent A), and vortex for 30 s.

[0140] 1.2. Centrifuge at 16000g at 4°C for 15min in a centrifuge, transfer the supernatant to a 1.5mL inlet EP tube, and concentrate the supernatant to dryness in a centrifugal concentration dryer at room temperature.

[0141] 1.3. Reconstitute with 5 μL of ultrapure water (reagent D) for analysis.

[0142] 2. Instrument testing

[0143] 2.1. Analytical instruments: UPLC Ultimate 3000system (Dionex) high performance liquid chromatograph; Q-Exactive high resolution mass spectrometer.

[0144] 2.2. Liquid phase conditions:

[0145] 2.2.1 The liquid chromatographic column was a Hypersil GOLD C18 chromatographic column (100 mm×2.1 mm, particle size 1.9 μm, Thermo...

Embodiment 3

[0158] Example 3 Stability analysis of tryptophanol and xanthoside in urine

[0159] The stability of tryptophanol and xanthoside levels in urine was evaluated using the method of Example 2 (interval time was 2 weeks). The results showed that the measured levels of tryptophanol and xanthoside in urine were stable ( figure 2 ), possessing properties as diagnostic / monitoring markers.

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Abstract

The invention belongs to the fields of analytical chemistry and clinical medicine, and discloses tryptophanol and xanthanin in urine as diagnostic markers for idiopathic male infertility and their application. The markers are tryptophanol and xanthanin, have high sensitivity and specificity in diagnosing idiopathic male infertility, and can be used to prepare reagents for diagnosing or monitoring idiopathic male infertility.

Description

[0001] Field of Invention [0002] The invention belongs to the field of analytical chemistry and clinical medicine, and relates to tryptophanol and xanthoside related to idiopathic male infertility in urine as biomarkers and a detection method and application based on UPLC-Q exactive MS. Background technique [0003] At present, the number of infertility patients among newlyweds in my country is far more than one million. Current research suggests that genetic factors such as Y chromosome AZF gene region sequence changes, DAZ gene copy number changes, sex hormone receptor gene sequence changes, and environmental factors such as exposure to harmful factors, nutrition and living habits are all related to male infertility. It is worth noting that a considerable part of male infertility presents with unexplained causes, i.e. idiopathic male infertility, which makes the diagnosis of idiopathic male infertility extremely difficult and delays the timing of treatment. The WHO's diag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 陈敏健丁兴旺管昱盛张怡云周昆徐菠庄婷钰张晓玲韩秀梅胡艳辉
Owner NANJING MEDICAL UNIV