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Yarn for cell culture support, ply twisted yarn containing same, and fabric containing same

A technology of cell culture and yarn, applied in cell culture support/coating, fabric, tissue culture, etc., can solve problems such as no effect, limited growth, proliferation, migration, differentiation, etc., to improve value-added rate and survival rate effect

Active Publication Date: 2022-02-01
AMOGREENTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned Patent Document 2 is a cell scaffold composed of a hydrophilized structure by nanofibrillating a biocompatible polymer material, which facilitates initial cell attachment. However, due to the characteristics of electrospinning, the nanofibers are randomly arranged, and it is difficult to The size of the formed stomata is 1 μm or more, and growth, proliferation, migration, and differentiation are limited after cell attachment, so there is a possibility that it may not bring appropriate effects when applied to a scaffold of cells with a specific shape

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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  • Yarn for cell culture support, ply twisted yarn containing same, and fabric containing same
  • Yarn for cell culture support, ply twisted yarn containing same, and fabric containing same
  • Yarn for cell culture support, ply twisted yarn containing same, and fabric containing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084]A spinning solution was prepared by dissolving polyvinylidene fluoride as a fiber forming component in DMAc / acetone (Acetone) as a mixed solution at 15% by weight. Utilize the electrospinning device to electrospin the above-prepared spinning solution, as the electrospinning conditions, the applied voltage is 25KV, the distance between the current collector and the spinning port is 25cm, the discharge volume is 0.05ml / hole, at RH65% Electrospinning was carried out under an environment of 30° C. to obtain a nanofiber web roll (Roll) with a width of 1.5 m, a weight of 5 g / ㎡, and a length of 500 m. Image 6 Part (a) is a photo of the prepared nanofibrous web by winding, Image 6 Part (b) is a photograph of a scanning electron microscope showing a nanofibrous web. Such as Image 6 As shown in part (b), the average diameter of the nanofibers forming the nanofiber web is about 230 nm.

Embodiment 2

[0085] Embodiment 2. Slitting yarn preparation and twisting

[0086] Such as Figure 7 Shown in part (a), after the roll of the nanofibrous web prepared in embodiment 1 is slitting for the first time with a width of 5mm, as Figure 7 As shown in part (b) of , the second precise slitting is carried out so that each width is 1.5mm to obtain the slitting yarn, and the winding photo of the slitting yarn prepared in the second precise slitting process is as follows Figure 7 as shown in part (c). Such as Figure 3a As shown, the width of the prepared slit yarn is 1.5mm. Using a 2-in-1 twister, the prepared slit yarn was Z-twisted so that the kink per minute was 700 T / M (T / M, twist / meter) to prepare a twisted yarn for cell scaffolds. Figure 8 Part (a) is a photograph showing the yarn wound with twisted yarn, Figure 8 Part (b) is a scanning electron micrograph of the surface of the twisted yarn, and it can be confirmed that two slit yarns are twisted to form multiple twists. ...

Embodiment 3～5

[0087] Embodiment 3~5. ply twisted yarn preparation

[0088] The slitting yarns prepared in Example 2 were plied with different yarns of nylon monofilament yarns of 20 deniers, polyester (Polyester) DTY of 30 deniers, and cotton of 60 counts respectively, and were plied by using a double twister to The condition of 1000T / M implements double twisting. Figure 9 A photograph and a scanning electron microscope photograph of the multi-twisted yarn prepared above are shown, respectively. Such as Figure 9 As shown, it can be confirmed that even in the case of preparing a plied twisted yarn of a slitting yarn and a different type of yarn, the plied twisted yarn is smoothly produced without fiber breakage, and it can be confirmed that a plurality of twists are formed by the plied twisted yarn.

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Abstract

The invention provides a yarn for a cell culture support. The yarn in one embodiment of the present invention includes: a plurality of yarns formed by twisting one or more single yarns; and fiber valleys formed in gap spaces between twists to provide three-dimensional growth spaces and moving paths for cells. Thus, the microenvironment suitable for the migration, proliferation, and differentiation of cultured cells can be realized by the yarn, thereby improving the cell proliferation rate and survival rate. Also, for each scaffold, the growth space and the migration path of the cells are made as similar as possible, so that a cell cluster with a more uniform shape can be easily realized. Further, the cells thus cultured can be cultivated to have a shape and result that are more suitable for in vitro realization of models or in vivo transplantation in animals, and can be widely used in cell culture fields such as bioreactors, cell culture containers, and kits for in vivo transplantation. Or various products used in the field of tissue engineering.

Description

technical field [0001] The present invention relates to a yarn for a cell culture scaffold, and more specifically, to a yarn for a cell culture scaffold, a ply-twisted yarn containing the same, and a fabric containing the same that achieve attachment, migration, and proliferation suitable for cultured cells , differentiated microenvironment, thereby improving the survival rate of cells, cells can proliferate three-dimensionally, and cell clusters can easily proliferate in a uniform shape, so that cell clusters can express physical properties similar to in vivo structures in physical, chemical, and biological ways. Background technique [0002] Recently, with the expansion of the use and application of cultured cells for disease treatment, interest and research on cell culture are actively being carried out. Cell culture is a technique of taking cells from an organism and culturing them in vitro, and differentiating the cultured cells into various body tissues such as skin, o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D02G3/26D02G1/02D02G3/44C12N5/00A61L27/38A61L27/44D03D15/41D03D15/50D03D15/33D03D15/283D03D15/47
CPCA61L27/38D02G3/448D02G1/0206C12N2535/00C12N2533/40C12N5/0068C12N2533/30A61L27/14A61L27/16C08L27/16A61L27/3834A61L27/3895A61L27/44C12N5/0062D02G1/02D02G3/26C12N2513/00D03D15/41D03D15/283D03D15/00C12N5/00C12N5/0656D02G1/0286D02G1/0293D02G3/02D10B2201/02D10B2321/042D10B2331/02D10B2331/04D10B2509/00
Inventor 徐寅踊张仙虎具松熙金灿李承勋
Owner AMOGREENTECH CO LTD
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