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Method for culturing magnetosome-producing iron oxidizing bacteria

A technology of iron-oxidizing bacteria and culturing method, applied in the biological field, can solve the problems of low yield of magnetosomes, harsh culturing conditions and the like

Inactive Publication Date: 2019-01-15
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the problems existing in the prior art, the present invention provides a method for cultivating magnetosome-producing iron-oxidizing bacteria, which mainly solves the problem of harsh culture conditions for existing iron-oxidizing bacteria and low magnetosome yields. question

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Fermentation culture of acidophilic iron oxidizing bacteria: Take acidophilic iron oxidizing bacteria liquid (the concentration of bacteria in the bacteria liquid is 5mg / L), and insert it according to the volume ratio of the bacterial liquid to the medium of 10%. In the fermenter, the culture temperature is 20℃, the oxygen supply is 1.8L / min, the culture time is 28h, the culture medium (calculated in 1L): ammonium sulfate 0.24g, potassium chloride 0.1g, dipotassium hydrogen phosphate 0.5g, sulfuric acid 0.5g magnesium, 0.01g calcium nitrate, 1.98g gluconic acid, add distilled water to 1000mL, use 1.0MH 2 SO 4 Adjust the pH to 2.0.

[0037] (2) Bacterial collection of acidophilic iron oxidizing bacteria: the grown bacterial liquid (log phase) is suction filtered with ordinary filter paper, and the filtrate is filtered through a microporous membrane with a diameter of 50 mm and 0.22 μm. Discard the filtrate. Take sulfuric acid with a pH of 2.0 to wash off the bacteria o...

Embodiment 2

[0041] (1) Fermentation culture of acidophilic iron oxidizing bacteria: Take acidophilic iron oxidizing bacteria liquid (bacteria concentration is 5mg / L), and put into the fermentation tank according to the volume ratio of bacterial liquid to medium of 10%. , Cultivation temperature is 30℃, oxygen supply is 3.6L / min, culture time is 28h, culture medium (calculated in 1L): ammonium sulfate 1.2g, potassium chloride 1.0g, dipotassium hydrogen phosphate 5.0g, magnesium sulfate 5.0 g, 0.1g calcium nitrate, 1.98g gluconic acid, add distilled water to 1000mL, adjust the pH to 2.0 with 1M sulfuric acid.

[0042] (2) Bacterial collection of acidophilic iron oxidizing bacteria: the grown bacterial liquid (log phase) is suction filtered with ordinary filter paper, and the filtrate is filtered through a microporous membrane with a diameter of 50 mm and 0.22 μm. Discard the filtrate. Take sulfuric acid with a pH of 2.0 to wash off the bacteria on the filter paper. Centrifuge the cell suspen...

Embodiment 3

[0046] (1) Fermentation culture of acidophilic iron oxidizing bacteria: Take acidophilic iron oxidizing bacteria liquid (the concentration of bacteria in the bacteria liquid is 5mg / L), and insert it according to the volume ratio of the bacterial liquid to the medium of 10%. In the fermenter, the culture temperature is 25℃, the oxygen flow rate is 5.6L / min, the culture time is 30h, the culture medium (calculated in 1L): ammonium sulfate 0.36g, potassium chloride 0.1g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.5 g, 0.01g calcium nitrate, 1.98g gluconic acid, add distilled water to 1000mL, use 1M sulfuric acid to adjust the pH to 2.0.

[0047] (2) Bacterial collection of acidophilic iron oxidizing bacteria: the grown bacterial liquid (log phase) is suction filtered with ordinary filter paper, and the filtrate is filtered through a microporous membrane with a diameter of 50 mm and 0.22 μm. Discard the filtrate. Take sulfuric acid with a pH of 2.0 to wash off the bacte...

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Abstract

The invention relates to a method for culturing magnetosome-producing iron oxidizing bacteria, comprising the following steps: inoculating the magnetosome-producing iron oxidizing bacteria into a culture medium, fermenting and culturing, wherein the culture temperature is 20 DEG C and the fermentation temperature is 20 DEG C; 25 DEG C. In addition to the culture temperature, the invention furtherconsiders the influence of the composition of the culture medium, the oxygen flux and other parameters on the production of ferrooxidizing bacteria magnetosomes, and finally realizes the purpose of significantly increasing the production rate of magnetosomes.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to a method for culturing magnetosomal iron oxidizing bacteria. Background technique [0002] Magnetotactic bacteria are bacteria that can form nano-scale magnetic particles-magnetosomes in the bacteria, and can move towards one end of the magnetic pole under the action of an external magnetic field. Magnetosomes have the characteristics of good biocompatibility, uniform particle size, high purity, strong magnetism and unique crystal form. They are a natural high-quality magnetic nanomaterial resource with great application potential. The magnetosomes extracted and purified from magnetotactic bacteria are single magnetic domain crystals. Due to their huge surface area to volume ratio, uniform particles, and the particles are covered by a layer of biofilm, they are not prone to aggregation and are not cytotoxic. This kind of nano-level magnetic particles has a wide range of potent...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 晏磊张爽范欣欣张玉王伟东
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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