A method for efficiently extracting polysaccharide from Hericium erinaceus by fermentation

A technology of Hericium erinaceus polysaccharides and fermentation technology, applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problem of low polysaccharide content, achieve the effects of less impurities, environmental friendliness, and improved extraction rate

Active Publication Date: 2019-01-15
福建拓天生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the yield of the extract and the con...

Method used

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  • A method for efficiently extracting polysaccharide from Hericium erinaceus by fermentation
  • A method for efficiently extracting polysaccharide from Hericium erinaceus by fermentation
  • A method for efficiently extracting polysaccharide from Hericium erinaceus by fermentation

Examples

Experimental program
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Embodiment 1

[0031] Step 1: Select fresh Hericium erinaceus fruiting bodies, remove inferior parts, wash with water, and use a refiner to refine;

[0032] Step 2: Treat the fruiting bodies of Hericium erinaceus after the pulping with microwaves for 5.5 minutes, then ultrasonic waves for 12.5 minutes, cool down to below 35°C, and set aside;

[0033] Step 3: Add water to the Hericium erinaceus sporocarp slurry treated in step 2, stir, and prepare Hericium erinaceus fruiting body mother liquor; the ratio of Hericium erinaceus fruiting body to water in the prepared mother liquor is 0.115 g / mL;

[0034] Step 4: Sterilize the mother liquor at 75°C for 55 minutes, inoculate the mixed strains in the Hericium erinaceus fruiting body mother liquor in the seed tank and cultivate them for 20 hours at 36°C, the total inoculation amount of the mixed strains is 7.5%, the ratio of the inoculation amount of lactic acid bacteria, saccharomyces, and Bacillus subtilis in the mixed strains is 3:1.25:1; mic...

Embodiment 2

[0042] Step 1: Select fresh Hericium erinaceus fruiting bodies, remove inferior parts, wash with water, and use a refiner to refine;

[0043] Step 2: Treat the fruiting bodies of Hericium erinaceus after the pulping with microwaves for 3 minutes, then ultrasonic waves for 5 minutes, cool down to below 35°C, and set aside;

[0044] Step 3: add water to the Hericium erinaceus fruiting body slurry after the treatment in step 2, stir, and prepare Hericium erinaceus fruiting body mother liquor; the ratio of Hericium erinaceus fruiting body to water in the prepared mother liquor is 0.03 g / mL;

[0045] Step 4: Sterilize the mother liquor at 65°C for 10 minutes, inoculate the mixed strains in the Hericium erinaceus fruiting body mother liquor in the seed tank and cultivate them for 12 hours at 35°C, the total inoculation amount of the mixed strains is 5%, the ratio of the inoculation amount of lactic acid bacteria, saccharomyces, and Bacillus subtilis in the mixed strains is 1:0.5...

Embodiment 3

[0053] Step 1: Select fresh Hericium erinaceus fruiting bodies, remove inferior parts, wash with water, and use a refiner to refine;

[0054] Step 2: Treat the fruiting bodies of Hericium erinaceus after refining with microwaves for 8 minutes, then use ultrasonic waves for 20 minutes, cool down to below 35°C, and set aside;

[0055] Step 3: add water to the Hericium erinaceus fruiting body slurry after step 2 treatment, stir, and prepare Hericium erinaceus fruiting body mother liquor; the ratio of Hericium erinaceus fruiting body to water in the prepared mother liquor is 0.2 g / mL;

[0056] Step 4: The mother liquor was sterilized at 85°C for 100min, and the mixed strain was inoculated in the mother liquor of Hericium erinaceus in the seed tank at 37°C for 28h, and the total inoculation amount of the mixed strain was 10%, the ratio of the inoculation amount of lactic acid bacteria, saccharomyces, and Bacillus subtilis in the mixed strains is 5:2:1; microscopically check the...

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Abstract

The invention discloses a method for efficiently extracting Hericium erinaceus polysaccharide by adopting a fermentation process, which comprises the following steps: selecting a fresh Hericium erinaceus fruit body, removing inferior parts, washing with water and grinding. The ground Hericium erinaceus fruiting body is firstly treated with microwave and then treated with ultrasonic wave, and the temperature is lowered for standby; Preparation of Hericium erinaceus fruiting body mother liquor; Sterilizing the obtained mother liquor, inoculating the mixed strain, fermenting and culturing, and then inactivating. The mixed strain comprises lactic acid bacteria, yeast and Bacillus subtilis; a liquid-solid separation is carry out on that fermentation broth, the filter residue is elute with clearwater, the eluting water is collect, the precipitate is standing and separated, the eluting water supernatant is taken and combined with the filtrate 1 to obtain liquid 2, the liquid 2 is added withethanol, the supernatant is standing and stratified, the supernatant is discarded, centrifugal separation is carried out, and the obtained substance is dried. The polysaccharide content of the extractobtained by the method is up to about 35% and the water solubility is good, and the yield of the extract is more than 20%, the impurities in the extract are few, the environment is friendly, and theextract can be produced on a large scale.

Description

technical field [0001] The invention relates to the field of plant processing, in particular to a method for efficiently extracting Hericium erinaceus polysaccharides through a fermentation process. Background technique [0002] Hericium erinaceus (scientific name: Hericium erinaceus), is a famous and precious traditional Chinese dish, with tender meat, fragrant taste, delicious taste. It is known as "mountain delicacy Hericium erinaceus, seafood bird's nest". This fungus of the Dendrite family has hairy spines on the surface of the cap, about 1 to 3 cm long. Its fruiting bodies are round and thick, white when fresh, light yellow to light brown after drying, and the base is narrow or slightly It has a short stalk, the upper part is enlarged, and the diameter is 3.5-10 cm. It looks like a golden monkey's head from a distance, so it is called "Hericium erinaceus", and it looks like a hedgehog, so it is also called "hedgehog fungus". A large number of medical studies have sho...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P19/04C12R1/125
CPCC12P19/04C12P39/00
Inventor 肖志勇方丽金郑秋芳叶艳鹏
Owner 福建拓天生物科技有限公司
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