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Use of loach polysaccharide as medicine or health food for treating immunodeficiency

A loach polysaccharide and immunodeficiency technology, which is applied in the directions of polysaccharide/gum-containing food ingredients, functions of food ingredients, drug combinations, etc., can solve the problems of low immune function, imperfect research, etc. Effects of treating immunodeficiency

Inactive Publication Date: 2019-01-18
深圳市生命谷生命科技研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, cyclophosphamide can also induce immunosuppression in patients, leading to low immune function in patients and forming an immunodeficiency state
[0005] Although loach polysaccharides are known to have immunomodulatory effects, the current research on this aspect is not perfect

Method used

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  • Use of loach polysaccharide as medicine or health food for treating immunodeficiency
  • Use of loach polysaccharide as medicine or health food for treating immunodeficiency
  • Use of loach polysaccharide as medicine or health food for treating immunodeficiency

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Detection of loach polysaccharides

[0042] Sulfuric acid-phenol method standard curve: Accurately weigh 100mg of glucose reference substance, place it in a 1000mL volumetric flask, add appropriate amount of water to dissolve, dilute to the mark, and shake well to obtain 0.1mg / ml glucose mother solution. Draw 0.4, 0.8, 1.2, 1.6, 2.0ml of glucose mother solution into 25ml stoppered test tubes, add water to make up to 2.0ml, add 1.0ml of 5% phenol aqueous solution and mix well. Then quickly add 5ml of concentrated sulfuric acid, shake well for 30s after 10min, and bathe in 30℃ water for 20min. Accurately pipette 150 μl of glucose solution into a 96-well plate, and measure the absorbance at a wavelength of 490 nm with the reagent blank solution as a reference. With the absorbance as the abscissa and the glucose concentration as the ordinate, draw a standard curve to obtain a regression equation: Y=0.1707X+0.0007(R 2 =0.9997).

[0043] Sample preparation: Accu...

Embodiment 2

[0046] Embodiment 2. Preparation of loach polysaccharide

[0047] method 1:

[0048] Put 2kg of cleaned loach in a stainless steel bucket with a cover, pour hot water of 1 times the weight of the loach at 70°C into the bucket, cover the lid, stir for 1 minute, filter, and collect the hot water; After cooling, add papain (800 u / mg) with 0.05% weight of loach, keep it in a water bath at 60°C for 6 hours; After standing still at 4°C for 4 hours, filter, and concentrate the filtrate with a 1000D nanofiltration membrane to about 200ml, add 4 times 95% ethanol to make the alcohol concentration greater than 70%, mix well, and stand at 4°C for 8 hours; Centrifuge at 10,000r / min for 10min to obtain a white or off-white precipitate, wash the precipitate twice with 95% ethanol, and then dry it at 60°C to obtain 1.5g of white powder. The sample is detected according to the method described in Example 1, and the polysaccharide content of loach is 19.9%.

[0049] Method 2:

[0050] Put ...

Embodiment 3

[0053] Example 3. Study on the Immunomodulatory Effect of Loach Polysaccharides

[0054] In this example, the immunomodulatory effect of polysaccharides from loach was studied.

[0055] 1 Experimental materials

[0056] 1.1 Materials and reagents

[0057] Cyclophosphamide (CY) was purchased from Jiangsu Hengrui Pharmaceutical Co., Ltd.; concanavalin A (ConA) was purchased from Sigma-Aldrich, USA; immunoglobulin kits (IgA, IgG, IgM), complement kits (C3, C4) were purchased from Nanjing Jiancheng Biological Company; mouse IL-2, TNF-α ELISA kits were purchased from Wuhan Boster Biological Engineering Co., Ltd.

[0058] 1.2 Instruments and equipment

[0059] Milli-Q Integral 5 purified water equipment (Merck Millipore Company); RCT basic type constant temperature magnetic stirrer (IKA Instrument Co., Ltd.); AL204 precision balance (Mettler-Toledo Instrument Co., Ltd.); Thermo 3111 carbon dioxide incubator (USA Thermo FisherScientific); CYTATION I5 microplate reader (BioTek, US...

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PUM

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Abstract

The invention relates to use of loach polysaccharide as medicine or health food for treating immunodeficiency. In a preferred embodiment, the immunodeficiency is a disease that can be treated by increasing the serum immunoglobulin content or a disease that can be treated by increasing the complement content. In another embodiment, the present invention also provides a health food for enhancing immunity comprising a loach polysaccharide.

Description

technical field [0001] The invention relates to the use of loach polysaccharide as medicine or health food for treating immunodeficiency. Background technique [0002] Loach (Misgurnus anguillicaudatus Cantor) is an animal of Loach family, and its meat or whole body is used as medicine, which is recorded in "Dictionary of Traditional Chinese Medicine". The synovial fluid secreted by the whole loach or the skin can be used as medicine, sweet, flat and non-toxic. Its main functions are to invigorate the spleen and Qi, nourish yin and strengthen yang, clear heat and detoxify, remove dampness, cure thirst, edema, jaundice, dysentery, impotence and other diseases. [0003] Loach polysaccharide is a neutral crude polysaccharide extracted from loach. The polysaccharide is composed of one high polysaccharide and one oligosaccharide. It is reported that it has anti-oxidation, immune regulation, and scavenging oxygen free radicals and other active functions. [0004] Cyclophosphamid...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P7/04A61P9/00A61P37/06A23L33/125
CPCA23L33/125A23V2002/00A61K31/715A61P7/04A61P9/00A61P37/06A23V2200/324A23V2200/326A23V2250/51
Inventor 刘牧龙左小峰蔡青青
Owner 深圳市生命谷生命科技研究院
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