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Horseradish peroxidase magnetic nano flower and preparation method and application thereof

A horseradish peroxidase and magnetic nanotechnology, applied in the field of nanomaterials, can solve the problems of inability to rapidly separate the immobilized enzyme, reduce the efficiency of reuse, etc., and achieve the improvement of activity and catalytic activity, improvement of reuse ability, and stability. Sexual improvement effect

Active Publication Date: 2019-01-18
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The defect of the nanoflowers directly formed by this method is that the immobilized enzyme cannot be quickly separated from the product, which reduces the efficiency of reuse.

Method used

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  • Horseradish peroxidase magnetic nano flower and preparation method and application thereof
  • Horseradish peroxidase magnetic nano flower and preparation method and application thereof
  • Horseradish peroxidase magnetic nano flower and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1: Preparation of magnetic composite microspheres

[0074] (1) Ferric oxide nanoparticles (Fe 3 o 4 )Synthesis

[0075] It was prepared by an improved hydrothermal method. 1.050 g of FeCl 3 ·6H 2 O, 3.454 g of NH 4 AC and 0.3 g of trisodium citrate dihydrate were dissolved in a single-necked flask (250 ml) filled with 60 mL of ethylene glycol, 80 o The oil bath of C was heated and stirred for 1 h to obtain a black homogeneous system, which was then transferred to a polytetrafluoroethylene-lined stainless steel autoclave (100 ml), and placed in a 180 o C oven reacted for 16 h, cooled to room temperature, magnetically separated the black product, washed several times with absolute ethanol until the supernatant had no color, and placed at 30 o C dried in a vacuum oven for 24 h.

[0076] (2) Polyacrylic acid modified ferric oxide (Fe 3 o 4 @MPS) Synthesis of microspheres

[0077] Weigh 0.1 g of Fe 3 o 4 Add 40 mL of ethanol, 10 mL of water and 1.5 mL...

Embodiment 2

[0084] Embodiment 2: Preparation of magnetic composite microspheres

[0085] (1) Ferric oxide nanoparticles (Fe 3 o 4 )Synthesis

[0086] It was prepared by an improved hydrothermal method. 1.650 g of FeCl 3 ·6H 2 O, 4.254 g of NH 4 AC and 0.5 g of trisodium citrate dihydrate were dissolved in a single-necked flask (250 ml) filled with 80 mL of ethylene glycol, 120 o The oil bath of C was heated and stirred for 1 h to obtain a black homogeneous system, which was then transferred to a polytetrafluoroethylene-lined stainless steel autoclave (100 ml), and placed in a 220 o C oven reacted for 16 h, cooled to room temperature, magnetically separated the black product, washed several times with absolute ethanol until the supernatant had no color, and placed at 30 o C dried in a vacuum oven for 24 h.

[0087] (2) Polyacrylic acid modified ferric oxide (Fe 3 o 4 @MPS) Synthesis of microspheres

[0088] Weigh 0.5 g of Fe 3 o 4 Add 40 mL of ethanol, 10 mL of water and 1.5 m...

Embodiment 3

[0095] Embodiment 3: Preparation of magnetic composite microspheres

[0096] (1) Ferric oxide nanoparticles (Fe 3 o 4 )Synthesis

[0097] It was prepared by an improved hydrothermal method. 1.350 g of FeCl 3 ·6H 2 O, 3.854 g of NH 4 AC and 0.4 g of trisodium citrate dihydrate were dissolved in a one-necked flask (250 ml) containing 70 mL of ethylene glycol, heated in an oil bath at 100 °C and stirred for 1 h to obtain a black homogeneous system, which was then transferred to polytetrafluoroethylene Vinyl fluoride-lined stainless steel autoclave (100 ml), placed in an oven at 200 °C for 16 h, cooled to room temperature, magnetically separated the black product, washed several times with absolute ethanol until the supernatant had no color, and placed in 30 o C dried in a vacuum oven for 24 h.

[0098] (2) Polyacrylic acid modified ferric oxide (Fe 3 o 4 @MPS) Synthesis of microspheres

[0099] Weigh a certain amount of 0.3 g of Fe 3 o 4 Add 40 mL of ethanol, 10 mL of...

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Abstract

The invention belongs to the technical field of nanometer materials, in particular to a novel horseradish peroxidase magnetic nanometer flower and a preparation method and application thereof. The invention adds HRP into the configured phosphate buffer solution. And then the synthesized magnetic composite microsphere Fe3O4 + PMG + IDA-Cu<2+> is added into that reaction solution, the temperature ofthe thermostat is adjusted. And the reaction is continue for a certain time to obtain magnetic nano flowers; And was used to degrade bisphenol A pollutant. The enzyme activity and catalytic activityof the nano flower prepared by the invention are obviously improved. And the stability under different conditions is also obviously improved. And the enzyme activity can reach 183% of free enzyme; Degradation of more than 90% bisphenol A in 25 minutes is much better than that of free enzymes.

Description

technical field [0001] The invention belongs to the technical field of nanometer materials, and in particular relates to a novel horseradish peroxidase magnetic nanoflower and its preparation method and application. Background technique [0002] Bisphenol A is an important organic material, which is widely used as a monomer material in life products such as synthetic polycarbonate plastics and epoxy resins. At the same time, the use of bisphenol A has also brought pollution, and the treatment of phenolic wastewater in the chemical industry has always been a problem. Many studies have shown that bisphenol A has adverse effects on humans and animals, and can cause a series of diseases including obesity, chronic diseases, endocrine disorders and cancer. Therefore, the removal of BPA from water has attracted extensive attention. The technologies currently applied to degrade bisphenol A mainly include activated sludge (carbon) method, biological fluidized bed method, microbial ...

Claims

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Application Information

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IPC IPC(8): C12N11/14B01J13/02C02F3/34C02F101/34
CPCB01J13/02C12N9/0065C12N11/14C12Y111/01007C02F3/342C02F2101/345
Inventor 罗鹏韩娟王赟王蕾李春梅毛艳丽倪良
Owner JIANGSU UNIV
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