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Preparation method of oxygen consumption protective layer on chlorella cell surface

A cell surface, chlorella technology, applied in the biological field, achieves good biocompatibility, promotes continuous hydrogen production, and is easy to operate

Active Publication Date: 2019-01-22
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still relatively few related studies on changing the green algae cells from oxygen production to hydrogen production in the atmospheric environment through cell surface engineering technology.

Method used

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  • Preparation method of oxygen consumption protective layer on chlorella cell surface
  • Preparation method of oxygen consumption protective layer on chlorella cell surface
  • Preparation method of oxygen consumption protective layer on chlorella cell surface

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specific Embodiment approach 1

[0016] Specific embodiment one: the preparation method of a kind of chlorella cell surface oxygen-consuming protective layer of this embodiment is specifically carried out according to the following steps:

[0017] 1. Cultivation of Chlorella pyrenoidosa cells: Select Chlorella pyrenoidosa as a biological template, and use TAP medium to cultivate in a light incubator with a temperature of 25-30°C under continuous light of 1200-4800LUX. When pyrenoid pellets Coating is carried out when the number of algae cells reaches the logarithmic growth phase to obtain Chlorella pyrenoidosa cells in the logarithmic growth phase;

[0018] 2. First use a NaCl solution with a concentration of 0.01 to 0.06mol / L to clean the logarithmic phase of the growth of Chlorella pyrenoidosa cells, and then use deionized water to clean the growth of the logarithmic phase of the Chlorella pyrenoidosa cells, Collect the cells by centrifugation, add the collected cells to the Tris buffer solution to obtain t...

specific Embodiment approach 2

[0022] Embodiment 2: This embodiment differs from Embodiment 1 in that: the OD value of the cell culture buffer in Step 2 is 2.0. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0023] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that the mass ratio of dopamine in step 2 to the cell culture buffer is 1 mg:0.375 mL. Others are the same as in the first or second embodiment.

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Abstract

The invention belongs to the technical field of bioengineering technology, and particularly relates to a preparation method of an oxygen consumption protective layer on the chlorella cell surface. According to the preparation method of the oxygen consumption protective layer on the chlorella cell surface, cell surface engineering technology is introduced to the green alga cell surface, green algacells generate hydrogen in an atmospheric environment through single coating of the green alga cell surface, and a functional protective layer is constructed manually on the cell surface to improve inherent nature and functions of a living body. The preparation method of the oxygen consumption protective layer on the chlorella cell surface comprises the steps that step one, culture of pyrenoid chlorella cells is carried out; step two, pyrenoid chlorella cells modified by polydopamine are prepared; and step three, pyrenoid chlorella cells coated with the polydopamine and laccase are prepared. The oxygen consumption protective layer with a bi-component structure is constructed on the pyrenoid chlorella surface. The oxygen consumption protective layer has better biocompatibility, can maintainown activity of the living body, enables the periphery of the pyrenoid chlorella to maintain the atmospheric environment and generate hydrogen, and has a certain defensive and protective effect on the living body when the external environment is unfriendly.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing an oxygen-consuming protective layer on the surface of chlorella cells. Background technique [0002] Energy is an important material basis for the survival and development of human society. With the depletion of fossil fuels, seeking and developing clean and non-polluting green alternative energy has become an important issue for scientific and technological workers. As a carbon-free and clean energy, hydrogen energy has the advantages of high calorific value, high energy conversion efficiency and clean and pollution-free combustion products. It is undoubtedly a very potential alternative energy source for fossil fuels. However, hydrogen energy is a secondary energy carrier and must be generated by other energy conversions. At present, the methods of hydrogen production include electrolysis of water to produce hydrogen, water gas method to produc...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 黄鑫苏东悦
Owner HARBIN INST OF TECH
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