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Germ plasma in-vitro preservation and rejuvenation method of pitaya

A technology of in vitro preservation and pitaya, applied in the field of fruit forest science, can solve the problems of consuming land, manpower, material resources, unable to provide large-scale seedlings, and being susceptible to virus and insect pests, and achieves space saving, strong resistance, and preservation. long time effect

Inactive Publication Date: 2019-01-25
LIANYUNGANG XIUJING LANDSCAPING ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing literature, most of the pitaya germplasm resources are preserved in the pitaya germplasm nursery by field planting, but this preservation method has some limitations: it is greatly affected by natural conditions or the external environment, and the preservation In the process, it is easy to be infected with viruses and insect pests, causing the degradation of germplasm resources; it consumes land, manpower, and material resources, but the reproduction coefficient is not high, and it is impossible to provide large-scale seedlings
At present, there is no relevant report on the in vitro preservation technology of pitaya germplasm. Therefore, the research on pitaya tissue culture is strengthened, and the method of germplasm preservation and rejuvenation of pitaya tissue culture is explored to establish a long-term storage time, stable genetic traits, and high survival rate after rejuvenation. It has important promotion value for the development of dragon fruit industry

Method used

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  • Germ plasma in-vitro preservation and rejuvenation method of pitaya
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  • Germ plasma in-vitro preservation and rejuvenation method of pitaya

Examples

Experimental program
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Embodiment 1

[0032] From 2011 to 2015, the pitaya germplasm Guihonglong No. 1, which originated from the pitaya plantation in Bobai County, Yulin, Guangxi, was selected for in vitro preservation of the germplasm according to the technical route of the present invention. The detailed steps are as follows:

[0033] (1) Acquisition and disinfection of dragon fruit explants

[0034] Select 'Guihonglong 1' 1-2 year-old young shoots with normal development and no pests and diseases, dip a little washing powder with a brush, rinse under running water for 30-40min; roughly cut the shoots into lengths of 3-4cm, width 1 For stems of -2cm, keep one thorn seat for each segment, rinse with purified water; then perform aseptic operation on an ultra-clean workbench, soak the stems in 75% alcohol for 30s, and then use 5% antifoni The solution was sterilized for 9 minutes, and finally rinsed with sterilized water for 3-5 times, and placed in a petri dish as an explant for later use;

[0035] (2) Adventiti...

Embodiment 2

[0040] The same germplasm source and technical route as in Example 1 were used for germplasm preservation in vitro, except that the germplasm preservation medium in step (3) was replaced with MS medium.

Embodiment 3

[0042] The pitaya germplasm material preserved for 4 years in embodiment 1 is carried out germplasm rejuvenation according to the technical route of the present invention, and detailed steps are as follows:

[0043] (1) Germplasm rejuvenation and proliferation

[0044] The surviving pitaya adventitious buds are transferred to the rejuvenation medium (the formula of the rejuvenation medium is: KNO 3 2300mg / L, NH 4 NO 3 1590mg / L, KH 2 PO 4 240mg / L, MgSO 4 ·7H 2 O 365mg / L, Ca(H 2 PO 4 ) 2 ·H 2 O 319mg / L, MnSO 4 4H 2 O 22mg / L, ZnSO 4 ·7H 2 O 8mg / L, H 3 BO 3 12.5mg / L, KI 0.7mg / L, CuSO 4 ·5H 2 O0.024mg / L, CoCl 2 ·6H 2 O 0.024mg / L, Na 2 MoO 4 2H 2 O 0.25mg / L, FeSO 4 ·7H 2 O 55mg / L, Na 2 -EDTA 62mg / L, inositol 66mg / L, lysine 5.5mg / L, serine 13.5mg / L, vitamin B1 0.4mg / L, vitamin B60.5mg / L, pantothenic acid 0.5mg / L, glutathione 37mg / L, complex peptide nucleic acid 0.35mg / L, candolol 0.75mg / L, calcium diphenylureasulfonate 1.0mg / L, tomato juice 44mL / L, succi...

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Abstract

The invention discloses a germ plasma in-vitro preservation and rejuvenation method of pitaya. The germa plasma in-vitro preservation and rejuvenation method comprises the following technical procedures: (1) acquiring and sterilizing a pitaya explant; (2) performing the adventitious bud induction culture; (3) performing the germ plasma culture; (4) performing the germ plasma rejuvenation proliferation; (5) performing the rooting culture; and (6) exercising and transplanting seedlings. The germ plasma preservation method can enable the pitaya to be stable in genetic traits, is simple to operate, achieves long preservation time, and provides technical guarantee for the preservation of excellent pitaya germ plasma; and the rejuvenation method can restore the vitality of the germ plasma storedfor a long term, is not limited by natural conditions, can provide a great amount of seedlings at any time, and has important industrial popularization value.

Description

technical field [0001] The invention relates to a method for in vitro preservation and rejuvenation of pitaya germplasm, in particular to a method for realizing long-term preservation and post-preservation rejuvenation and propagation of dragon fruit germplasm by using tissue culture technology, and belongs to the field of fruit forest science and technology. Background technique [0002] Dragon fruit (scientific name: Hylocereus undatus 'Foo-Lon'), also known as red dragon fruit, dragon pearl fruit, xianmi fruit, and jade dragon fruit, is a cultivar of the genus Liangtianchi in the cactus family. Its fruit is oblong or oval, with Red, fleshy, with egg-shaped scales with sharp tips, the whole appearance looks like an angry red fireball, hence the name dragon fruit. Dragon fruit is native to the tropical regions of Central America, and is mainly distributed in China and Vietnam in Asia. Pitaya is mainly distributed in Taiwan, Hainan, Guangdong, Guizhou, Guangxi, Fujian, Yu...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 秦楠楠张翠朱慧玲
Owner LIANYUNGANG XIUJING LANDSCAPING ENG CO LTD