Double-network hydrogel material containing antibacterial nano calcium phosphate particle filling material and preparation method thereof

Abstract

The invention discloses a double-network hydrogel material containing an antibacterial nano calcium phosphate particle filling material and a preparation method thereof. The method comprises the following steps: -poly(glutamic acid) modified copper-doped nano-calcium phosphate is prepared into an aqueous suspension, and then an initiator ammonium persulfate and a crosslinking agent N,N'-methylenebisacrylamide are added to adjust the pH to 5.0-6.5, the sodium alginate is added and stirred evenly, then tetramethylethylenediamine is added, and stirred for a reaction, and the material is washed and dried to obtain the double-network hydrogel material containing the antibacterial nano-calcium phosphate particle filling material. The copper-doped calcium phosphate is modified by poly(glutamic acid), which not only improves the replacement rate of copper ions in calcium phosphate, but also strengthens the bonding, effectively slows the overflow of the copper ions, and slows down the cell toxicity at an initial release stage on the basis of antibacterial action. The antibacterial calcium phosphate particles are nanospheres, and the particle size is mainly between 10 nm and 70 nm.

Description

Technical field: [0001] The invention belongs to the technical field of biological materials, in particular to a double network hydrogel material containing antibacterial nano calcium phosphate particle fillers. Background technique: [0002] Copper is closely related to bone health. When the human body lacks copper, it can easily lead to osteoporosis, which makes bones prone to fractures. The main reason is that copper can affect collagen maturation. Copper deficiency will reduce the synthesis of collagen fibers in bone, resulting in inhibition of epiphyseal growth, loose new bone structure, fragility of long bones and termination of bone growth. Supplementation of copper in the culture medium of osteoblasts can increase the cross-linking of collagen fibers, which is beneficial to the synthesis of collagen. Osteogenesis is the result of a combination of factors including angiogenesis and cell induction. Copper ions promote angiogenesis. More importantly, copper has good a...

AI Technical Summary

Problems solved by technology

At present, the preparation method of antibacterial agent using hydroxyapatite as the carrier is mainly the ion exchange method. The disadvantage of this method is that the combination of copper ions and the carrier is weak, resulting in rapid dissociation of copper ions and the long-term antibacterial properties of the product cannot be maintained. In addition, use If the concentration of free copper ions is too high in the early stage, it will be toxic, interfere with bone metabolism, inhibit collagen synthesis, and lead to a decrease in collagen in the backbone and epiphysis