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Application of multiprotein composition and congenital heart disease screening kit

A technology of congenital heart disease and composition, applied in the field of molecular biology, can solve the problems of not achieving the expected effect, not having many relationships and in-depth and comprehensive research, and achieve the effect of great application value

Active Publication Date: 2021-10-01
TEDA INT CARDIOVASCULAR HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although many researches on etiology and genetics related to PDA have been carried out, there are not many and in-depth and comprehensive researches on the relationship between disease-related protein substances and diseases, which is far from reaching people's expected results

Method used

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  • Application of multiprotein composition and congenital heart disease screening kit
  • Application of multiprotein composition and congenital heart disease screening kit
  • Application of multiprotein composition and congenital heart disease screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0169] 1. Collection of Human Plasma Samples

[0170] Blood samples were collected from all congenital heart disease patients (experimental group) and normal control children (control group) in the morning before surgery under the condition of fasting>10h. 2ml of whole blood was collected from each patient's peripheral vein, and immediately centrifuged at 2500rpm for 10 minutes to separate the upper layer of plasma. The separated plasma was then divided into several equal parts, placed in plasma collection tubes, and stored in a -80°C refrigerator for testing.

[0171] 2. Proteomic detection

[0172] 1) Plasma proteome detection

[0173] Proteins in each mixed sample of the two groups were detected by iTRAQ combined with multidimensional liquid chromatography-mass spectrometry (LC-MS / MS) (Applied Biosystems, Foster City, CA).

[0174] 2) Sample preparation

[0175] a) Purchase a high-abundance protein removal kit (ProteoExtractTM Albumin / IgG Removal Kit, CALBIOCHEM, USA) t...

Embodiment 2

[0238] Enzyme-linked immunosorbent assay (ELISA)

[0239] The platelet factor 4, von Willebrand factor, fibrinogen, collagen, protein-associated serine protease 2 and fibronectin screened out in the proteomics results were verified in the plasma samples of the verification group by ELISA method. Experimental principle: The antibody of the protein to be tested is pre-embedded at the bottom of the 96-well plate. After the standard and sample are added, the protein to be tested will bind to the antibody. After removal of unbound substrate, a biotin-conjugated antibody to the protein of interest is added. After washing, anti-biotin conjugated horseradish peroxidase-labeled antibody (HRP) was added, and unbound HRP was removed by washing. After adding a chromogenic reagent to terminate the reaction, measure the absorbance of the liquid.

[0240] A platelet factor 4 protein detection kit, which comprises:

[0241] Multi-well plate coated with platelet factor 4 antibody

[0242] ...

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Abstract

Application of polyprotein composition and congenital heart disease screening kit. The present invention uses modern biological technology and bioinformatics analysis to study the detection and function of platelet factor 4, von Willebrand factor, fibrinogen, collagen, protein-related serine protease 2 and fibronectin, and find the joint application It has the purpose of predicting the patent ductus arteriosus of congenital heart disease, can be applied to the preparation of targeted drugs to intervene related diseases early, and can also be applied to the preparation of congenital heart disease screening kits, which are useful for preventing the patent ductus arteriosus. This disease has great potential application value.

Description

technical field [0001] The invention relates to the field of molecular biology, especially the application of the polyprotein composition and the screening kit for congenital heart disease. Background technique [0002] Birth defects are the number one cause of death of infants and children, and congenital heart disease has become the number one disease of birth defects in my country. How to reduce the incidence of congenital heart disease and improve the curative effect of congenital heart disease has become an important challenge to the health of our population in the new century. Internationally, birth defects are also the number one cause of threats to children's survival and quality of life. The "Global Birth Defects Report" released by the United States in 2006 estimated that there are more than 8 million new birth defects in the world every year, and 90% of them occur in low- and middle-income countries. Every year, more than 3.3 million children under the age of 5 ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/543A61K45/00A61P9/00
CPCA61K45/00A61P9/00G01N33/543G01N33/68
Inventor 何国伟侯海涛张茜王君杨沁
Owner TEDA INT CARDIOVASCULAR HOSPITAL
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