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Electrochemical method for detecting beta-lactam antibiotics

A technology for lactams and antibiotics, which is applied in the electrochemical field of detecting beta-lactam antibiotics, can solve problems such as mutagenesis, imbalance of bacterial flora in the human body, allergic reactions, etc., and achieves rapid and accurate detection and good application prospects. , the effect of simple and convenient operation

Active Publication Date: 2019-02-15
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antibiotic residues can cause the following problems: allergic reactions; increased bacterial resistance; carcinogenic, teratogenic, mutagenic effects; imbalance of flora in the human body and affect the quality of the ecological environment, etc.
[0006] However, at present, there are no relevant research reports on the preparation of β-lactam antibiotic electrochemical biosensors by combining polymethylene blue, NDM-1 enzyme, and electrochemical methods at home and abroad.

Method used

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  • Electrochemical method for detecting beta-lactam antibiotics
  • Electrochemical method for detecting beta-lactam antibiotics
  • Electrochemical method for detecting beta-lactam antibiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Sprinkle a small amount of 1 μm, 0.3 μm and 0.05 μm polishing powder (Al 2 o 3 ), the glassy carbon electrode was first polished on 1 μm, 0.3 μm and 0.05 μm polishing powder in turn, and the surface of the glassy carbon electrode was cleaned with deionized water, and then the electrode was moved into an ultrasonic water bath, followed by distilled water, ethanol and Rinse with distilled water for 2 min and dry at room temperature. Put the polished glassy carbon electrode into the phosphate buffer solution of pH=6 for activation, use the platinum wire electrode as the counter electrode, the silver silver chloride electrode as the reference electrode, and the polished glassy carbon electrode as the working electrode to construct three electrodes The structure, using the Chenhua electrochemical workstation to activate the electrode for 20 cycles, the activation method is cyclic voltammetry, the conditions are: the initial potential -0.6V, the highest potential 2V.

[002...

Embodiment 2

[0029] Sprinkle a small amount of 1 μm, 0.3 μm and 0.05 μm polishing powder (Al 2 o 3 ), the glassy carbon electrode was first polished on 1 μm, 0.3 μm and 0.05 μm polishing powder in turn, and the surface of the glassy carbon electrode was cleaned with deionized water, and then the electrode was moved into an ultrasonic water bath, followed by distilled water, ethanol and Rinse with distilled water for 2 min and dry at room temperature. Put the polished glassy carbon electrode into the phosphate buffer solution of pH=6 for activation, use the platinum wire electrode as the counter electrode, the silver silver chloride electrode as the reference electrode, and the polished glassy carbon electrode as the working electrode to construct three electrodes The structure, using the Chenhua electrochemical workstation to activate the electrode for 20 cycles, the activation method is cyclic voltammetry, the conditions are: the initial potential -0.6V, the highest potential 2V.

[003...

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Abstract

The invention discloses an electrochemical method for detecting beta-lactam antibiotics. The surface of a glassy carbon electrode is modified by polymethylene blue, New Delhi metal-beta-lactamase-1 (NDM-1 enzyme) and glutaraldehyde. The preparation method comprises: activating bare electrodes; performing electropolymerization of methylene blue; preparing working electrodes; mixing 4 [mu]L of enzyme and 2 [mu]L of 1.0% glutaraldehyde onto the surface of the electrode and drying it; placing the electrode in penicillin sodium and ampicillin solution and deceting by differential pulse voltammetryto obtain linear ranges of 0.001 ng to 10 [mu]g and 0.01 ng to 10 [mu]g, respectively. The detection limits were 0.0057 ng mL-1, 0. 011 ng mL-1. The advantages are high sensitivity, low detection limit, low cost, simple operation, and reusability. The electrochemical method can be applied to the detection of beta-lactam antibiotics, and provide a promising technology for the detection of beta-lactam antibiotics.

Description

technical field [0001] The invention relates to an electrochemical method for detecting beta-lactam antibiotics, in particular to an electrochemical method for detecting penicillin sodium and ampicillin. Background technique [0002] Antibiotics are widely used in livestock farming, and there is overuse, resulting in antibiotic residues in meat and dairy products (such as milk), of which β-lactam antibiotics are the most common. Antibiotic residues can cause the following problems: allergic reactions; increased bacterial resistance; carcinogenic, teratogenic, mutagenic effects; imbalance of the flora in the human body and affect the quality of the ecological environment. At present, although antibiotic residues in food have been widely concerned, due to the limitation of detection methods, the problem of antibiotic residues in animal products in my country has not been solved, so it is extremely important to find a fast and accurate detection method. [0003] Conventional a...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3271
Inventor 王虹苏罗瑞平牛效迪修毅朱丽郭玲韩保庆黄延军
Owner JILIN UNIV
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