Electrochemical method for detecting beta-lactam antibiotics

A technology for lactams and antibiotics, which is applied in the electrochemical field of detecting beta-lactam antibiotics, can solve problems such as mutagenesis, imbalance of bacterial flora in the human body, allergic reactions, etc., and achieves rapid and accurate detection and good application prospects. , the effect of simple and convenient operation

Active Publication Date: 2019-02-15
JILIN UNIV
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  • Application Information

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Problems solved by technology

Antibiotic residues can cause the following problems: allergic reactions; increased bacterial resistance; carcinogenic, teratogenic, mutagenic effects; imbalance of flora in the human body and affect the quality of the ecological environm

Method used

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  • Electrochemical method for detecting beta-lactam antibiotics
  • Electrochemical method for detecting beta-lactam antibiotics
  • Electrochemical method for detecting beta-lactam antibiotics

Examples

Experimental program
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Example Embodiment

[0024] Example 1

[0025] Sprinkle a small amount of 1μm, 0.3μm and 0.05μm polishing powder (Al 2 O 3 ), first grind the glassy carbon electrode on 1μm, 0.3μm and 0.05μm polishing powder in turn, clean the surface of the glassy carbon electrode with deionized water, then move the electrode into an ultrasonic water bath, use distilled water, ethanol and Wash with distilled water for 2 min and dry at room temperature. The polished glassy carbon electrode was activated in pH=6 phosphate buffer, the platinum wire electrode was used as the counter electrode, the silver-silver chloride electrode was used as the reference electrode, and the polished glassy carbon electrode was used as the working electrode to construct three electrodes. The electrode was activated by Chenhua electrochemical workstation for 20 cycles. The activation method was cyclic voltammetry, and the conditions were as follows: the initial potential was -0.6V, and the highest potential was 2V.

[0026] The acti...

Example Embodiment

[0028] Example 2

[0029] Sprinkle a small amount of 1μm, 0.3μm and 0.05μm polishing powder (Al 2 O 3 ), first grind the glassy carbon electrode on 1μm, 0.3μm and 0.05μm polishing powder in turn, clean the surface of the glassy carbon electrode with deionized water, then move the electrode into an ultrasonic water bath, use distilled water, ethanol and Wash with distilled water for 2 min and dry at room temperature. The polished glassy carbon electrode was activated in pH=6 phosphate buffer, the platinum wire electrode was used as the counter electrode, the silver-silver chloride electrode was used as the reference electrode, and the polished glassy carbon electrode was used as the working electrode to construct three electrodes. The electrode was activated by Chenhua electrochemical workstation for 20 cycles, and the activation method was cyclic voltammetry, and the conditions were: initial potential -0.6V, maximum potential 2V.

[0030] The activated glassy carbon electro...

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Abstract

The invention discloses an electrochemical method for detecting beta-lactam antibiotics. The surface of a glassy carbon electrode is modified by polymethylene blue, New Delhi metal-beta-lactamase-1 (NDM-1 enzyme) and glutaraldehyde. The preparation method comprises: activating bare electrodes; performing electropolymerization of methylene blue; preparing working electrodes; mixing 4 [mu]L of enzyme and 2 [mu]L of 1.0% glutaraldehyde onto the surface of the electrode and drying it; placing the electrode in penicillin sodium and ampicillin solution and deceting by differential pulse voltammetryto obtain linear ranges of 0.001 ng to 10 [mu]g and 0.01 ng to 10 [mu]g, respectively. The detection limits were 0.0057 ng mL-1, 0. 011 ng mL-1. The advantages are high sensitivity, low detection limit, low cost, simple operation, and reusability. The electrochemical method can be applied to the detection of beta-lactam antibiotics, and provide a promising technology for the detection of beta-lactam antibiotics.

Description

technical field [0001] The invention relates to an electrochemical method for detecting beta-lactam antibiotics, in particular to an electrochemical method for detecting penicillin sodium and ampicillin. Background technique [0002] Antibiotics are widely used in livestock farming, and there is overuse, resulting in antibiotic residues in meat and dairy products (such as milk), of which β-lactam antibiotics are the most common. Antibiotic residues can cause the following problems: allergic reactions; increased bacterial resistance; carcinogenic, teratogenic, mutagenic effects; imbalance of the flora in the human body and affect the quality of the ecological environment. At present, although antibiotic residues in food have been widely concerned, due to the limitation of detection methods, the problem of antibiotic residues in animal products in my country has not been solved, so it is extremely important to find a fast and accurate detection method. [0003] Conventional a...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3271
Inventor 王虹苏罗瑞平牛效迪修毅朱丽郭玲韩保庆黄延军
Owner JILIN UNIV
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