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Periodontal healing baicalin-containing drug combination

A technology of baicalin and drugs, applied in the field of pharmacy, can solve problems such as inability to promote alveolar bone regeneration, drug resistance, and imbalance of organism flora

Inactive Publication Date: 2019-02-19
WUXI CHILDRENS HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Drugs include systemic medication and topical medication. Most systemic medications are oral antibiotics, and local medications are mostly chemically synthesized antibacterial rinses, gargles, and gel drugs for local use. However, long-term systemic and local application of antibiotics will lead to imbalance of the body’s flora , and produce drug resistance, also unable to promote alveolar bone regeneration
Paleo Dental Ointment, a drug commonly used in the treatment of periodontitis, is mainly composed of minocycline hydrochloride, and its safety in children has not yet been determined

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 Detection of baicalin monomethoxy polyethylene glycol-polycaprolactone nanoparticles

[0014] The concentration of baicalin in the nanospheres was determined by high performance liquid chromatography. Accurately weigh 5 mg of baicalin nanoparticles freeze-dried powder and dissolve in 5 ml of distilled water, then centrifuge the solution at 12,000 rpm and 4° C. for 20 min, and take 20 μl of the supernatant into a chromatographic column for determination. Another weighed equivalent amount of baicalin nanoparticles was dissolved in 5ml of methanol, and the solvent was evaporated to dryness with slight heat, then dissolved in the mobile phase and filtered through a 0.45 μm membrane, then transferred to a 10ml volumetric flask and fixed to volume with the mobile phase to obtain a sample solution. A 20 μl sample was used for chromatographic analysis. The chromatographic column is Agilent C-18, 5μ, 200mm×4.6mm, the mobile phase is 48 / 52 / 0.05 (v / v / v) (methanol / double...

Embodiment 2

[0016] Cytological experiment effect of embodiment 2 baicalin nanoparticles

[0017] ① Isolation, culture and identification of periodontal ligament stem cells

[0018] Primary culture of periodontal ligament cells: collect healthy periodontal and caries-free fresh premolars extracted for orthodontic needs, wash them with PBS 3 times, scrape 1 / 3 of the periodontal ligament tissue from the root, and use enzymes to decompose the tissue blocks Cultured in this method, changing the medium every 3 days until the cells swim out from around the tissue block, and the cells are passaged when they reach 80% confluency.

[0019] Cloning, culture, and purification of PDLSCs Take the first generation cells in the logarithmic growth phase, seed them on a 96-well plate at a density of 1-2 cells / well, and culture them routinely for 7-14 days until cell clones appear (the number of cells ≥ 50 is the criterion) Then expand the cultivation.

[0020] Identification: The second-generation clone-...

Embodiment 3

[0027] Example 3 Effect of baicalin mPEG-PCL nanoparticle gel on experimental periodontitis in rats

[0028] ①Establishment of rat experimental periodontitis model: SD rats were anesthetized by intraperitoneal injection of 0.38ml / 100g body weight with 10% chloral hydrate. The buccal-palatal gingiva of the first molar on the side was instilled with 50 μL 1g / L LPS at the bottom of the gingival sulcus to establish an experimental periodontitis alveolar bone resorption model, once every other day, a total of 7 times.

[0029] ②The rats with periodontitis in the experimental group were given baicalin mPEG-PCL nanoparticle gel locally daily, and the rats with periodontitis in the control group were given iodine glycerin locally. Four weeks later, after the rats were sacrificed, the jaws of the experimental side were fixed, decalcified, embedded, and cut into paraffin blocks. Histological observations were made on the experimental group and the control group.

[0030] The results s...

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Abstract

The invention provides a periodontal healing baicalin-containing drug combination. The periodontal healing baicalin-containing drug combination is composed of mPEG-PCL (methoxy poly(ethylene glycol)-poly(epsilon-caprolactone)) and baicalin and can be applied to treatment on young permanent tooth injury, inhibit local inflammatory response and promote regeneration of periodontal tissues.

Description

technical field [0001] The invention belongs to the field of pharmacy, and in particular relates to the application of nano particles containing baicalin in periodontal healing of young permanent teeth after trauma. Background technique [0002] For adolescents who are in the growth and development stage, due to their large amount of exercise but lack of self-protection awareness, maxillofacial injuries are extremely prone to occur. The incisors are located in the front of the mouth and are relatively more vulnerable to injury. Permanent central incisor trauma has become one of the common diseases of maxillofacial injuries in the Department of Stomatology of Children's Hospital. Once the front teeth are damaged, it will affect the patient's ability to cut food, and even the pronunciation and facial appearance will be affected, causing great physical and mental damage to the child. [0003] After the trauma of young permanent teeth, in addition to the damage of the tooth bod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/34A61K31/7048A61K9/06A61P1/02
CPCA61K9/0063A61K9/06A61K31/7048A61K47/34A61P1/02
Inventor 徐贤寅沈波朱金晓邹茵薛黛朱文婷李晓丹傅晓峰蔡欣茹
Owner WUXI CHILDRENS HOSPITAL
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