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Warfarin individualized drug-related gene polymorphism detection kit and its preparation method and application

A gene polymorphism, detection kit technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of easily polluted flux, cumbersome operation, and long time.

Active Publication Date: 2021-02-19
XIAMEN ZEESAN BIOTECH
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  • Description
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Problems solved by technology

[0023] The purpose of the present invention is to provide a simple and fast, high sensitivity, high reliability, low cost based on Fluorescence PCR melting curve technology warfarin individualized drug-related gene polymorphism detection kit and its preparation method and application

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  • Warfarin individualized drug-related gene polymorphism detection kit and its preparation method and application
  • Warfarin individualized drug-related gene polymorphism detection kit and its preparation method and application
  • Warfarin individualized drug-related gene polymorphism detection kit and its preparation method and application

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specific Embodiment

[0116] The prepared kit of the present invention is used to detect polymorphic sites of warfarin individualized drug-related genes in case samples, and the specificity and accuracy of the kit to detect samples are investigated.

[0117] according to figure 1 Described sample detection flow process, utilize the case sample [peripheral blood of the genotype of the polymorphism base site of 14 known individualized drug-related genes of the present invention, through Lab-Aid nucleic acid (DNA) separation kit (Xiamen Zhishan Biotechnology Co., Ltd.) for the extraction of genomic DNA, which has been sequenced to verify the genotype of the polymorphic site of the relevant gene] for detection, and set a standard control and negative control at the same time, including the following steps:

[0118] 1) Using each sample to be tested and the standard control as templates, prepare PCR reaction solution: 25 μL PCR reaction solution includes: 5 μL DNA template (negative control is water), 1...

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Abstract

The invention relates to a kit for detecting gene polymorphisms related to warfarin individualized medicine and its preparation method and application, which relate to the detection of gene polymorphisms. The detection kit is provided with a box body, a partition, a box cover, a WP PCR mixed solution bottle, a WP enzyme mixed solution bottle, a WP standard control bottle, a WP negative control bottle, an amplification reagent and a control reagent. Prepare the amplification reagent; prepare the control reagent, the control reagent includes MP standard control and MP negative control; set the amplification reagent and the control reagent in the box body, and obtain the warfarin individualized drug-related gene polymorphism detection kit . The warfarin individualized drug-related gene polymorphism detection kit is applied in detecting the genotype of the warfarin individualized drug-related gene polymorphism site. Simple and fast, a single reaction can detect multiple sites, and takes a short time.

Description

technical field [0001] The invention relates to the detection of gene polymorphisms, in particular to a kit for detecting gene polymorphisms related to warfarin individualized medication and its preparation method and application. Background technique [0002] Warfarin is a coumarin-based oral anticoagulant that exerts its anticoagulant effect by inhibiting the interconversion of vitamin K and its 2,3-epoxide (ie, vitamin K epoxide). Warfarin is currently the most widely used oral anticoagulant in clinical practice. It has been used clinically for nearly 60 years. It is mainly used to prevent and treat venous thrombosis, myocardial infarction, ischemic shock, pulmonary embolism and other thrombotic diseases and cardiac Artificial valve replacement, artificial blood vessel transplantation, etc. Due to the narrow therapeutic window of warfarin (there is a risk of thrombosis if the dose is insufficient, and fatal bleeding may occur if the dose is overdose), the dose difference...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12Q1/6858
CPCC12Q1/6858C12Q1/6883C12Q2600/106C12Q2600/156C12Q2531/113C12Q2563/107C12Q2527/107
Inventor 黄秋英李庆阁
Owner XIAMEN ZEESAN BIOTECH
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