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A method for rapid detection of pesticide residues by extracting samples with organic solvents

A technology for organic solvents and pesticide residues, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, so as to improve the recovery rate, eliminate the influence, and improve the accuracy and sensitivity.

Active Publication Date: 2022-01-14
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, when reporting various methods for the rapid detection of pesticides by enzymatic reactions, they are all limited to aqueous solvents. Whether it is the production of standard samples or the extraction of samples, the solvents are mostly water or aqueous buffers.

Method used

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  • A method for rapid detection of pesticide residues by extracting samples with organic solvents
  • A method for rapid detection of pesticide residues by extracting samples with organic solvents
  • A method for rapid detection of pesticide residues by extracting samples with organic solvents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] figure 1 It is a flow chart of the method for rapid detection of pesticide residues by using a microfluidic paper chip to extract samples with an organic solvent. The paper chip can adopt various configurations of the microfluidic paper chip, as long as the detection area is required to be the same size. In the embodiment of the present invention, the most common radial Pozider-shaped chip configuration is adopted.

[0080] figure 1 Among them, the paper chip is provided with a rice-shaped hydrophilic channel network, including a central area and 8 hydrophilic channels evenly surrounding the central area; one end of each hydrophilic channel is connected to the central area, and the end far away from the central area is set as Reaction detection area

[0081] The central zone is circular with a diameter of 5 mm. Each channel has a length of 4mm and a width of 1.5mm;

[0082] The reaction detection zone is a circle with a diameter of 5 mm.

[0083] Eight hydrophilic ...

Embodiment 2

[0100] In order to compare the detection effect of the inventive method (carry out pretreatment extraction with organic solvent) and conventional enzyme inhibition detection method (carry out sample extraction with water), and check the reliability of the detection method of the present invention in actual sample detection, the inventor concludes Lettuce was used as a model, and water and acetonitrile were used as extraction solvents respectively, and the recoveries of four representative pesticides with different polarities, such as phoxim, carbaryl, carbofuran, and methamidophos, were determined at a single spiked level. The specific operation is as follows:

[0101] (2-1) Weigh four crushed head lettuce samples, 10g each, count as a, b, c, and d samples, put them into 4 centrifuge tubes in turn, and add them according to the corresponding concentrations shown in Table 1 Corresponding to the pesticide standard solution and vortex mixing, 4 spiked samples of lettuce were obta...

Embodiment 3

[0115] The method established by the invention can not only be used to detect solid samples, but also can realize rapid determination of pesticide residues in various water bodies and beverages.

[0116] In the national limit standard, the limit level of organophosphorus and carbamate pesticide residues in samples such as environmental and domestic water and fruit juices is below 0.05 mg / kg, which is far lower than the detection limit of existing quick detection methods. Conventional detection methods are difficult to perform simple on-site rapid screening tests. In the method of the present invention, since the enzyme inhibition detection can be compatible with organic solvents, the extraction and concentration of the target pesticides can be realized by using water-immiscible organic solvents for extraction and reducing the solvent / sample ratio, thereby improving the detection sensitivity as a whole . This is especially true for the rapid detection of poorly water-soluble car...

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Abstract

The invention provides a method for quickly detecting pesticide residues by extracting samples with an organic solvent: (1) extracting samples with an organic solvent, vortex mixing, centrifuging after standing, and taking the supernatant as a sample to be tested; (2) extracting the sample with an organic solvent Prepare the standard solution with solvent; add the sample to be tested, the blank control and / or the standard solution dropwise to each detection area of ​​the paper chip, and after drying naturally, add the cholinesterase solution dropwise and let it stand for 5-20 minutes to obtain the sample addition solution. (3) Enzyme activity detection: detect the residual activity of cholinesterase on the paper chip after sample addition, perform semi-quantitative determination or make an enzyme activity index working curve and calculate the level of pesticide content in the sample to be tested. The invention eliminates the influence of the solvent on the enzyme activity of the added enzyme reagent, extracts the sample with an organic solvent, and uses the enzyme reaction to perform rapid detection of pesticide residues. The organic solvent extracts the sample can greatly improve the extraction efficiency, and the recovery rate is greatly improved compared with water extraction. The detection accuracy and sensitivity are improved.

Description

technical field [0001] The invention belongs to the field of food detection, and in particular relates to a method for sample pretreatment and enzyme inhibition reaction detection in rapid detection of pesticide residues. Background technique [0002] The rapid detection technology of pesticide residues based on enzyme activity inhibition is widely used in the screening and detection of agricultural product quality and safety. The principle of this reaction is that the enzyme can catalyze the reaction of the probe substrate and produce a detectable photochemical or electrochemical signal, but the pesticide residue will inhibit the enzyme activity, resulting in a decrease or even disappearance of the substrate reaction signal, thus quickly screening out Whether there are pesticide residues in agricultural products. [0003] However, biological enzymes generally have high activity in aqueous solvents, and the presence of organic solvents will significantly affect the activity...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 郝振霞刘新鲁成银金莉莉
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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