Culturing method of gerbera jamesonii novel variety

A cultivation method and technology for new varieties, applied in the directions of flower cultivation, botanical equipment and methods, culture medium, etc., can solve the problems of inability to maintain the excellent characteristics of the mother plant, affecting the promotion of new varieties, slow propagation of seedlings, etc. The effect of culture cycle, strong repeatability and good quality

Active Publication Date: 2019-02-22
云南喜迎春科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Tissue culture is an effective way to use transgenic technology to genetically improve organisms and cultivate fine varieties. It has been widely used in the rapid reproduction of fruit trees and flowers, and the cultivation of virus-free plants. Thi

Method used

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  • Culturing method of gerbera jamesonii novel variety
  • Culturing method of gerbera jamesonii novel variety

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A cultivation method for a new variety of gerbera, comprising: explant callus induction, proliferation cultivation, rooting cultivation, seedling hardening and transplanting, specifically comprising the following steps:

[0016] 1) Callus induction of explants: take the lateral buds of a new variety of Gerbera chrysanthemum with strong growth, disinfect with 75% alcohol for 30 seconds, then disinfect with 0.1% mercury chloride solution for 5 minutes, rinse twice with sterile water, and take the stems The tip part was cut off, the length was 0.3mm, inserted into MS solid medium without exogenous hormones, and cultured under the conditions of temperature 22°C, light intensity 2500lx, and light time 24h / d to obtain callus tissue in vitro Under the action of suitable nutrients, hormones and other external conditions, the living plant cells in the state can show totipotency and develop into a complete plant. The induction of callus is to induce the cells in the differentiated...

Embodiment 2

[0021] A cultivation method of a new gerbera variety, specifically comprising the following steps:

[0022]1) Callus induction of explants: take the lateral buds of a new variety of Gerbera chrysanthemum with strong growth, disinfect them with 85% alcohol for 40 seconds, then disinfect them with 0.1% mercuric chloride solution for 10 minutes, rinse them twice with sterile water, and take the stems. The tip part was cut off, the length was 0.5mm, inserted into the MS solid medium without exogenous hormones, and cultured under the conditions of temperature 24°C, light intensity 2800lx, and light time 24h / d to obtain callus;

[0023] 2) Proliferation culture: insert the callus into the proliferation medium, and under the conditions of temperature 26°C, light intensity 1300lx, and light time 13h / d, cultivate new shoots to obtain clustered shoots after proliferation. Contains the following ingredients: MS+6-benzylaminoadenine 0.5mg / l+KT kinetin 0.3mg / l+naphthaleneacetic acid 0.2mg / ...

Embodiment 3

[0027] A cultivation method of a new gerbera variety, specifically comprising the following steps:

[0028] 1) Callus induction of explants: take the lateral buds of a new variety of Gerbera chrysanthemum with strong growth, disinfect with 80% alcohol for 35 seconds, then disinfect with 0.2% mercuric chloride solution for 8 minutes, finally rinse with sterile water for 3 times, and take the stems The tip part was cut off with a length of 0.4mm, inserted into MS solid medium without exogenous hormones, and cultured under the conditions of temperature 25°C, light intensity 3000lx, and light time 24h / d to obtain callus;

[0029] 2) Proliferation culture: insert the callus into the proliferation medium, and under the conditions of temperature 28°C, light intensity 1500lx, and light time 14h / d, cultivate new shoots to obtain clustered shoots after proliferation. Contains the following ingredients: MS+6-benzylaminoadenine 0.4mg / l+KT kinetin 0.3mg / l+naphthaleneacetic acid 0.2mg / l+aga...

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Abstract

The invention discloses a culturing method of a gerbera jamesonii novel variety. The method comprises the steps that after lateral buds of the gerbera jamesonii novel variety are taken and sterilized,bud tip parts are taken to be grafted to a culture medium, and culturing is conducted to obtain a callus; the callus is grafted to a propagation culture medium to by cultured to obtain a clump shoot;after the clump shoot is segmented, the clump shoot is grafted to a rooting culture medium, and a rooting seedling is obtained; the rooting seedling is hardened and taken out, sterilized and transplanted to a nutritious culture medium to be cultured, and a gerbera jamesonii novel variety young seedling is obtained; the propagation culturing medium contains sodium p-toluenesulfonate and ethyl decanoate. The invention provides a culturing method of the gerbera jamesonii novel variety, the method is simple, the implementation is easy, the operability is high, the repeatability is high, the emergence rate of vitrification seedlings in tissue culturing is reduced, the culturing efficiency is effectively improved, the purposes of efficient and rapid propagation are achieved, and the gerbera jamesonii novel variety young seedling has the advantages of stable hereditary character, low variation, excellent quality, strong root system and the increasing number of tiller.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for cultivating a new variety of gerbera. technical background [0002] Gerbera, also known as Gerbera, is a perennial herb of the genus Gerbera in the Compositae family. It is popular among people for its rich colors, large flowers, tall and straight flower branches, and bright colors. Its high yield can be produced every year. It is the main cut flower in the world. One, widely used in cut flower species. Gerbera is native to southern Africa. In 1887, Jameson of England introduced it to the UK, and it was widely planted in many countries and regions in the world. As one of the top five cut flowers in the world, my country has been cultivating them on a large scale since the 1980s. The main production areas are Shanghai, Guangdong, Yunnan, Beijing, Liaoning, Hainan and other places. [0003] With the continuous development of the flower market, the amount of...

Claims

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Application Information

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IPC IPC(8): A01G22/60A01G2/00A01G24/28A01G24/25A01G24/15A01G24/12C05G3/00
CPCA01G2/00A01G22/60A01G24/12A01G24/15A01G24/25A01G24/28C05F11/10C05G5/20C05G3/00
Inventor 鲁元学马振兴桂大萍陈丹蒲佳红
Owner 云南喜迎春科技有限公司
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