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Method for extracting high-purity collagen from bovine bone

A technology of pure collagen and extraction method, which is applied in the field of high-purity collagen extraction from bovine bone, can solve the problems of wide molecular weight distribution, high protein yield, and many impurity proteins, and achieves short extraction time, improved yield and purity. , the effect of improving biological activity

Active Publication Date: 2019-02-22
GUIZHOU MOUNT FANJING ECOLOGICAL AGRI INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzymatic extraction is to control a certain temperature and PH, use protease to enzymolyze raw materials, and extract collagen after impurity removal and fine filtration. The cycle is short, 1-24h ends, the protein yield is high, the amino acid is less damaged, and it is environmentally friendly. The disadvantage is the molecular weight Wide distribution, high cost, more miscellaneous proteins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for extracting high-purity collagen in bovine bone, comprising the steps of:

[0029] S1 washes the fresh beef bone with clear water to remove the meat residue on its surface, and then washes it with a citric acid solution with a mass concentration of 10%;

[0030] S2 Put the cleaned bovine bone into the reactor, add an ethanol solution with 7 times the mass of the cleaned bovine bone and a mass concentration of 50%, ventilate to a temperature of 130°C, stop the ventilating and adjust the pressure to 95MPa, and conduct a closed reaction for 2 hours Finally, high-speed stirring to form a slurry, and passing through a 60-mesh sieve to obtain bovine bone pulp;

[0031] S3 uses a high-pressure homogenizer to process bovine bone pulp. The material is ejected from the flow-limiting gap, turbulently sheared, and collided to make the material ultra-fine into extremely small particles to form a uniform bovine bone pulp;

[0032] S4 Add an organic acid solution with a m...

Embodiment 2

[0041] A method for extracting high-purity collagen in bovine bone, comprising the steps of:

[0042] S1 washes the fresh beef bone with clear water to remove the meat residue on its surface, and then washes it with a citric acid solution with a mass concentration of 7%;

[0043] S2 Put the cleaned bovine bone into the reactor, add 4 times the mass of the cleaned bovine bone and an ethanol solution with a mass concentration of 45%, ventilate to a temperature of 110°C, stop the ventilating and adjust the pressure to 80MPa, and conduct a closed reaction for 1h Finally, high-speed stirring to form a slurry, and passing through a 60-mesh sieve to obtain bovine bone pulp;

[0044] S3 uses a high-pressure homogenizer to process bovine bone pulp. The material is ejected from the flow-limiting gap, turbulently sheared, and collided to make the material ultra-fine into extremely small particles to form a uniform bovine bone pulp;

[0045] S4 Add an organic acid solution with a mass co...

Embodiment 3

[0054] A method for extracting high-purity collagen in bovine bone, comprising the steps of:

[0055]S1 washes the fresh beef bone with clear water to remove the meat residue on its surface, and then washes it with a citric acid solution with a mass concentration of 8%;

[0056] S2 Put the cleaned bovine bone into the reactor, add the ethanol solution with 5 times the mass of the cleaned bovine bone and 48% mass concentration, ventilate to a temperature of 120°C, stop the ventilating and adjust the pressure to 85MPa, and conduct a closed reaction for 1.5 After h, stir again at high speed to form a slurry, and pass through a 60-mesh sieve to obtain bovine bone pulp;

[0057] S3 uses a high-pressure homogenizer to process bovine bone pulp. The material is ejected from the flow-limiting gap, turbulently sheared, and collided to make the material ultra-fine into extremely small particles to form a uniform bovine bone pulp;

[0058] S4 Add an organic acid solution with a mass conc...

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Abstract

The invention relates to the technical field of bio-collagen preparation, in particular to a method for extracting high-purity collagen from bovine bone. The method includes the following steps: the step S1 of washing fresh bovine bone clean; the step S2 of putting the washed bovine bone into a reactor, adding an ethanol solution, ventilating and conducting sealed reaction to form bovine bone pulp; the step S3 of treating the bovine bone pulp with a high pressure homogenizer to form uniform bovine bone pulp; the step S4 of adding an acidic solution to the bovine bone pulp, mixing uniformly, conducting ultra-high pressure impinging stream treatment, closed reactor treatment, high-voltage pulsed electric field treatment and closed reactor treatment, conducting solid and liquid separation with a filter press to obtain an extract; the step S4 of conducting ultrafiltration treatment on the extract to collect an ultrafiltrate and conducting spray drying to obtain the collagen. The purity ofthe collagen is 98% or more, the yield of the collagen is 90% or more, the biological activity of the collagen is improved, the absorption rate of the human body is increased to more than 98.5%, and the extraction time is short.

Description

technical field [0001] The invention relates to the technical field of biological collagen preparation, in particular to a method for extracting high-purity collagen from bovine bone. Background technique [0002] Collagen is a natural biopolymer synthesized by animal cells. It is widely found in animal skin, bone, cartilage, teeth, tendons, ligaments and blood vessels. The role of the body. Collagen has a stable nature, and it is difficult to decompose it under general processing temperature and short-term heating, so it is difficult to be absorbed by the human body, and the utilization rate of food is low. [0003] At present, the commonly used extraction methods include chemical methods and enzymatic degradation methods. The chemical method is to use chemical reagents such as acid and alkali to break the peptide chain of protein molecules at a certain temperature to form small molecular substances. The prior art, such as the Chinese invention patent with the patent num...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/34
CPCC07K14/78
Inventor 王小清蒋宇
Owner GUIZHOU MOUNT FANJING ECOLOGICAL AGRI INC
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