A strain of Aspergillus oryzae za112 and its application
A ZA112, Aspergillus oryzae technology, applied in the direction of fungi, microorganisms, microorganisms, etc., can solve the undiscussed problems of Aspergillus oryzae hydrolysis and dissolution rate, increase production cost, high production cost, etc., achieve rich aspergillus spores, save production cost, The effect of high utilization rate of raw materials
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Embodiment 1
[0038] Embodiment 1 Aspergillus oryzae strain mutagenesis
[0039] The spores of the mature strain of Huyao 3.042 were made into spore suspension, and the spore suspension was subjected to ultraviolet mutagenesis, and the spore suspension after ultraviolet mutagenesis was diluted with a 10-fold gradient method, and the spore suspension was suspended with sterile physiological saline. diluted to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Concentration, made spore dilution.
[0040] Take 0.2 mL of spore dilution and spread it on the wheat bran plate medium, and incubate at 30°C in the dark for 4 days. During the cultivation process, observe and record the growth of the colony, the speed of the formation of the transparent circle, the size of the transparent circle, and measure and calculate the circle-diameter ratio. A total of 32 single colonies were observed and recorded during this process, among which 5 strains had a fast growth rate and a large circle-to-diameter ratio...
Embodiment 2
[0043] Example 2 Screening of mutagenized strains
[0044] (1) Primary screening of mutagenized strains
[0045] The 5 strains of target strains obtained by the above mutagenesis were activated and transferred to the test tube slant medium, and cultured for four days. Take the mature slant and inoculate them in the culture medium of Erlenmeyer flasks for expansion. Among them, the culture medium is prepared from bran, soybean flour, flour, and water; the culture medium and process control of the triangular flask adopt conventional methods.
[0046] Select bacterial strains LA001, LA002, LA003, LA004, and LA005 with normal hyphae growth and spores to detect the koji index (using conventional detection methods). The detection results are shown in Table 1.
[0047] Table 1 The quality analysis results of strain screening Erlenmeyer flasks
[0048] strain Spore count Neutral protease activity Arabinase activity As3.042 1.00 1.00 1.00 LA001 0.85 1....
Embodiment 3
[0061] Example 3 Aspergillus oryzae ZA112 strain genetic stability detection
[0062] The aspergillus oryzae bacterial strain ZA112 that embodiment 2 is screened out is continuously subcultured 10 generations through bean juice slant medium, observes the growth situation of each generation strain, and the 1st generation, the 5th generation and the 10th generation slant bacterial classification are according to embodiment 1. Said method is expanded step by step to prepare triangular flask koji and koji making, and its genetic stability is judged. If the error of spore count, neutral protease activity and arabinase activity error in ten generations is within 10% error range, it shows that the bacterial strain is genetically stable. sex is good. See Table 4 for specific data.
[0063] Table 4 Koji-making test results of subculture stability of Aspergillus oryzae strain ZA112
[0064] Test items ZA112 first generation ZA112 Generation 5 ZA112 10th generation As...
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