Extracting agent for extracting DNA from insect materials and application thereof

Abstract

The invention discloses an extracting agent for DNA extraction and application thereof. The extracting agent for DNA extraction is composed of Tris-base, NaCl, PVP and Tween-20, and can rapidly extract a trace amount of DNA from insect materials, and only the extracting agent, instead of other liquid, needs to be disposed in the sample extraction process. The reagents adopted by the extracting agent are all commonly used chemical reagents in a laboratory, and are low in cost, so that the cost is reduced; and the extracting agent is stable in component, does not need to be prepared when needed,can be repeatedly used, and can be stored at room temperature for at least 3 months. At the same time, the process of extracting the trace amount of DNA of insect samples is simple and rapid, and theextraction can be completed within 30 s. The DNA extracted by the extracting agent has stable quality and can be directly used for subsequent experimental detection. The biggest advantage of the method is that only one extracting agent is used, the extraction process can be completed within 30 s, and the extracted DNA has stable quality and can be directly applied to subsequent experimental detection.

Description

Technical field [0001] The patent of the present invention relates to the field of biotechnology, in particular to an extractant for extracting trace DNA from insects and its application. Background technique [0002] With the development of modern molecular biology and informatics, various DNA-based gene detection technologies have developed rapidly, such as allele specific amplification (ASA), restriction fragment length polymorphism analysis ( Restrietion fragment length polymorphism (RFLP), PCR single / double strand conformation polymorphism (SSCP), random amplified polymorphic DNA (RAPD), loop-mediated isothermal Amplification technology (Loop-mediated isothermal amplification, LAMP), etc., are widely used in the fields of insect species identification and identification, resistance level monitoring, insect biological characteristics research, etc., for the protection of insect biodiversity, scientific management of harmful insects, and beneficial insects in China Reasonable...

AI Technical Summary

Problems solved by technology

There are many traditional DNA methods, such as the traditional classic phenol-chloroform method, etc. The DNA obtained by these methods is of high purity and can meet the requirements of various tests, but the operation is cumbersome and time-consuming, and the reagents used have certain toxicity

For example, the salt extraction method, the yield is relatively low, and the purity is poor

Such as silica gel membrane adsorption column method, magnetic bead method, etc., need to be eluted repeatedly, and the operation is still relatively complicated

Domestic and foreign biological companies have also developed a variety of commercial nucleic acid DNA extraction kits, and some companies have developed a rapid micro-DNA method, but there are still problems such as complicated operations, long time, more samples, and unstable extraction results.

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