A device and method for detecting cecropin b using magnetic iron oxide nanoparticles as signal amplifiers
A magnetic iron oxide and signal amplifier technology, applied in the directions of magnetic particle immunodiagnostic reagent carriers, measuring devices, instruments, etc., can solve the problem of high analysis cost by high performance liquid chromatography, difficulty in determining the titration end point of potentiometric titration, and objective environmental impact. Large and other problems, to achieve the effect of high detection sensitivity, small impact on the objective environment, and fast detection speed
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Embodiment 1 4
[0043] EXAMPLES One to 4 Blank Detection Device and Polarization Detection
Embodiment 1
[0045] 1) Cut the slide into a slide and a slide, and then soak the upper slide and the slide with the Piranha solution at a temperature of 80 ° C, so that the upper slide and the surface of the slide have a hydroxyl group, and then rinsed Blow dry, where the Piranha solution is 2 SO 4 With h 2 O 2 The volume ratio is 7: 3;
[0046] 2) Immersion of the upper slide into the DMOAP aqueous solution for 30 min to 50 min, and then rinse it, then blow it; at the same time, the slide is placed in an ethanol solution of 60 ° C, and then blows dry, wherein the ethanol solution is added to DMOAP. The volume ratio is 3: 1, the volume fraction of the DMOAP aqueous solution is 0.2%, wherein the volume fraction of APTES is 3%, and the volume fraction of DMOAP is 1%;
[0047] 3) Immerse the slide into the GA aqueous solution for 30-50 min, then purge it, blow dry; where the volume fraction of the Ga aqueous solution is 2%;
[0048]4) Take the Tiansworm B antibody solution to the surface of the s...
Embodiment 2
[0055] 1) Cut the slide into a slide and a slide, and then soak the upper slide and the slide with a Piranha solution at a temperature of 100 ° C, so that the upper slide and the surface of the slide have a hydroxyl group, and then rinsed Blow dry, where the Piranha solution is 2 SO 4 With h 2 O 2 The volume ratio is 7: 3;
[0056] 2) Immersion of the upper slide into the DMOAP aqueous solution for 30 min to 50 min, then rinse, blow dry; simultaneously placed the slide in a ethanol solution of 70 ° C, then blow it, then blow dry, where Aptes and DMOAP in ethanol The volume ratio was 3: 1, the volume fraction of the DMOAP aqueous solution was 0.2%, of which the volume fraction of APTES was 5%, and the volume fraction of DMOAP was 1.7%;
[0057] 3) Immersion of the slide into the GA aqueous solution for 30-50 min, then purge, blow dry; wherein the volume fraction of the Ga aqueous solution is 1.5%;
[0058] 4) Take the Tiansworm B Antibody solution to the surface of the slide, then ...
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