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A high-efficiency enrichment method for functional substances in broccoli sprouts

A technology of broccoli sprouts and functional substances, which is applied in the fields of seed and rhizome treatment, agriculture, application, etc., can solve the problems of unused broccoli, achieve the effect of short enrichment cycle, scientific process, and improve nutritional value

Active Publication Date: 2021-07-20
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are domestic reports on the effects of environmental stress on plant glucosinolate content, for example, Guo Liping's doctoral thesis "Heat Shock, Hypoxia and JA and ABA Regulate the Formation Mechanism of Sulforaphane in Broccoli Sprouts", Tian Ming's Doctoral thesis "Study on the Effects of Selenium Treatment on the Synthesis and Metabolism of Glucosinolates in Broccoli", Ding Yi's master thesis "Effects of Selenium-Sulphur Interaction on the Growth and Antioxidant Quality of Chinese Cabbage Plants" and thesis "Broccoli Sulfur, Selenium Metabolic Competition and Its Effects on Health Function Research Progress" and others did not conduct heat shock treatment and selenium-sulfur interaction at the same time and did not control the treatment time period, or did not use broccoli as the experimental raw material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The particles were full, high germination rate, and unbeatted broccoli as a raw material, which was immersed in 1.5% sodium hypochlorite solution, then washed with distilled water until neutral pH, placed in 5 times (V / W) deion Soak 4 h in water in water; placed the soaked Broccoli seed particles in the germinated machine, germinate 5d at 30 ° C, and the spray concentration of 0.01 mM sodium selenite and 3 mM sulfate was mixed 10ml during the first 2D germinate. After germination 2d, the bud seedlings were placed at 40 ° C for 1 time, 20 min each time, during the concentration of 0.1 mM sodium selenite and 6 mM sulfate mixed aqueous Aqueous Zilang bud seedlings were brought for 2 minutes every 24 hours. The spray liquid flow is 10 mL / min. The sulfur in the broccoli seedlings is about 51 mg / g dw, and the content of selenium is about 26 μg / g dw.

Embodiment 2

[0021] The granules were full, the germination rate was high, and the unbeatted broccoli was selected from 1.5% hypochlorite solution for 15 min, then washed with distilled water until neutral pH, placed in 6 times (V / W) deion Soak 4 h in water in water; placed the soaked Broccoli seed particles in the germinated machine, germinate 5d at 30 ° C, and the spray concentration of 0.05 mm sodium sodium sulfate and 2 mM sulfate mixed aqueous solution during the first 2D germinate. After germination 2D, the bud seedling was placed at 40 ° C for 1 time, 15 min each time, during which aqueous concentration of 0.1 mM sodium selenite and 10 mm sulfate mixed aqueous solution was 2min for 2 min every 16h. The spray liquid flow is 6 ml / min. The glucoside content of the sulfur in the broccoli seedlings is about 56 mg / g DW, and the content of selenium is about 24 μg / g dw.

Embodiment 3

[0023] The granules were full, the germination rate was high, and the unbeatted broccoli was selected from 1.5% hypochlorite solution for 15 min, then washed with distilled water until neutral pH, placed in 6 times (V / W) deion Soak 4 h at 30 ° C in water; placed the soaked broccoli seed particles in the germinated machine, germination of 7d, and spray concentrations per 24 mm sodium sodium sodium sulfate during 30 ° C, 5 ml of sodium sodium sulfate, zinc sulfate. After 2 days, the bud seedling was placed at 37 ° C for 1 time, 20 min each time, during which the concentration of 1 mm sodium selenite and 6 mm sulfate mixed aqueous solution was 2min, sprayed. The shadder flow rate is 10 ml / min. The glucoside content of the sulfur in the broccoli seedlings is about 60 mg / g dW, and the content of selenium is about 33 μg / g dw.

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Abstract

The invention relates to a high-efficiency enrichment method for functional substances in broccoli sprouts, belonging to the field of food biotechnology. The specific steps are: within 5-7 days of germination of broccoli seeds, they germinate normally in the first two days without applying stress. During the growth process of the sprouts two days after the seeds germinate, place them under high temperature stress at 35-60°C once a day, 15-25 minutes each time, and spray stress with a mixed aqueous solution of 0.1-1mM sodium selenite and 6-10mM zinc sulfate every 16-24 hours for 1-2 minutes, and the spray flow rate is 5-10mL / min. The raw material of the invention has wide application range, simple operation and low production cost. After broccoli sprouts are treated with heat shock and selenium-sulfur interaction stress, the content of glucosinolate and selenoamino acid in the broccoli sprouts produced is 5-10 times that of before treatment, which can realize the Efficient enrichment of functional substances such as amino acids.

Description

Technical field [0001] The present invention relates to a high-efficiency enrichment method of functional substances in a broccoli branch, belonging to the field of food biotechnology. Background technique [0002] Thiogucoside (referred to as sulfide) is a nitrogen-containing and sulfur secondary metabolite, thioside (GRA) and its metabolite radish (ICTS) have been confirmed to have a flavor and nutrient ingredients of cruciferous vegetables. , Plant self-protection mechanisms and human physical health have close relationships. Broccoli belongs to two bonus plants in one year, and a large number of studies have confirmed that they can effectively enrich organic selenium. Compared with other plant species, 薹 薹 is not only effective to synthesize the ability of selenoids, selenoids and selenoids, but also in 450 organisms containing sulfides in nature, their thioside content is the most abundant. Studies have shown that radish sulfide content is 10 to 100 times the ripened broccol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00
CPCA01C1/00
Inventor 尹永祺韩宇朱振宇沈敏熙程雨薇方维明
Owner YANGZHOU UNIV
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