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Preparation method of chitosan-graphene oxide supported polyprenol-fullerene nanocomposite

A nanocomposite, polyprenol technology, applied in the field of biomedicine, can solve the problems of insoluble in water, low bioavailability, affecting the development of biological activity research preparations, etc., and achieves good water dispersibility and obvious biological activity effect. , the effect of improving bioavailability

Inactive Publication Date: 2019-03-01
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polyprenol has strong hydrophobicity, is difficult to dissolve in water, and has low bioavailability in humans and animals, which affects the research on its biological activity and the development of related preparations

Method used

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  • Preparation method of chitosan-graphene oxide supported polyprenol-fullerene nanocomposite
  • Preparation method of chitosan-graphene oxide supported polyprenol-fullerene nanocomposite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Preparation of polyprenol-fullerene nanoemulsion

[0025] Slowly add 10 mL of toluene solution containing 0.1% fullerene and 2% polyprenol dropwise to 50 mL of emulsifiers, polyethylene glycol octylphenyl ether and n-octanol (mass ratio 1:1, HLB value 9.2) In the mixture, and under the condition of heating at 40°C, stir evenly at a speed of 500r / min to obtain an oil phase; after the system is stable, continue to add 20% deionized water under stirring at a speed of 500r / min for phase inversion, and the system will change from clarified to It becomes turbid, then add 5 times the amount of deionized water under the condition of heating at 70°C, assisted by 500W ultrasonic power and stir at the speed of 12000r / min for 0.25h at the same time, the system becomes transparent or translucent to obtain polypentane Enol / Fullerene Nanoemulsions.

Embodiment 2

[0026] Embodiment 2 Preparation of chitosan-graphene oxide composite dispersion

[0027] Slowly add 100 mL of acetic acid solution containing 1% chitosan dropwise into 40 mL of aqueous solution containing 0.1% graphene oxide, heat at 60°C and stir at a speed of 500 r / min for 10 h, then slowly add 4 mL of ammonia solution containing 0.5% folic acid Add it dropwise, heat at 70°C and stir at a speed of 500r / min for 10h, and then the chitosan-graphene oxide composite dispersion is obtained;

Embodiment 3

[0028] Example 3 Preparation and performance measurement results of chitosan-graphene oxide-loaded polypentenol-fullerene nanocomposites

[0029]Slowly add the polypentenol-fullerene nanoemulsion obtained in 10mL embodiment 1 into the chitosan-graphene oxide composite dispersion liquid obtained in 100mL embodiment 2, and heat at 50°C at a speed of 500r / min Stir for 5 hours, then slowly add a solution containing 1% sodium tripolyphosphate, heat at 60°C and stir at a speed of 500r / min for 10 hours, and then freeze and vacuum dry for more than 24 hours to obtain chitosan-graphene oxide-supported Polyprenol-fullerene nanocomposites.

[0030] The chitosan-graphene oxide-loaded polypentenol-fullerene nanocomposite prepared by the above method was tested for performance, stability and biological activity. The results showed that the particle size distribution of the material in the emulsion reached 27.3- 82.1nm, with an average particle size of 44.9nm; the drug loading rates of poly...

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Abstract

The invention provides a preparation method of a chitosan-graphene oxide supported polyprenol-fullerene nanocomposite. The preparation method is characterized by including (1) adding a methylbenzene solution of fullerene and polyprenol dropwise to an emulsifier, heating and stirring to obtain an oil phase; adding a certain amount of deionized water for phase inversion after a system is stabilized,then increasing the temperature, adding a large amount of deionized water, assisting stirring ultrasonically to obtain a polyprenol / fullerene nano-emulsion; (2) slowly adding a chitosan acetic acid solution into an aqueous graphene oxide solution dropwise, heating and stirring, slowly adding a folic acid ammonia aqueous solution dropwise into the aqueous graphene oxide solution, heating and stirring to obtain a chitosan-graphene oxide compound dispersion solution; (3) heating and stirring the two solutions obtained in the steps (1) and (2) for mixing, slowly adding a sodium tripolyphosphate solution dropwise to the mixture, heating and stirring and conducting vacuum freeze drying to obtain the chitosan-graphene oxide supported polyprenol-fullerene nanocomposite.

Description

technical field [0001] The invention relates to a preparation method of a chitosan-graphene oxide-loaded polypentenol-fullerene nanocomposite, which belongs to the field of biomedicine. technical background [0002] Polyprenols (GBP) is based on C 5 Lipid compounds with isopentenyl as the structural unit are important active ingredients in the synthesis of glycoproteins in the cell membrane of organisms, participate in various metabolisms in organisms, and have a wide range of biological activities. Polyprenol is a key product of the biosynthesis of cell membrane glycoproteins in organisms. It is distributed in the cell membrane in the form of free alcohol or phosphate, and can participate in various physiological metabolic activities in organisms. Therefore, polyprenol can be used as a good drug delivery carrier. Using polyprenol as a good biological cell membrane carrier to send drugs into cells to participate in metabolism can better improve the bioavailability and targ...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K31/765A61K47/36A61K47/04A61P35/00
CPCA61K9/143A61K9/146A61K31/765A61P35/00
Inventor 王成章陶冉张昌伟
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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