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A kind of preparation method of puerarin extract

A puerarin and extract technology, applied in the field of preparation of puerarin extract, can solve the problems of increasing the difficulty of puerarin monomer preparation, low yield of puerarin, low puerarin content, etc., to achieve environmental protection, The effect of less impurities and easy preparation

Active Publication Date: 2021-12-28
绵阳膳意生物科技有限公司
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Problems solved by technology

[0004] At present, most puerarin is extracted from Pueraria root by water boiling or alcohol heat reflux extraction technology. The content of puerarin in the obtained extract is low, between 10-15%, and it contains more mechanical impurities and other impurities, which increases the subsequent puerarin. Difficulty in the preparation of puerarin monomer, the yield of puerarin is very low, and the cost is high

Method used

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  • A kind of preparation method of puerarin extract
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Examples

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Embodiment 1

[0039] Medium preparation: comprehensive potato medium: peeled potato 200g, glucose 20g, agar 20g, KH 2 PO 4 , Thiamine 8mg, MgSO 4 ·7H 2 O 1.5g. Preparation method: Peel the potatoes, cut them into small pieces, add 1000mL of water and boil for 20 minutes, then filter out the potato pieces, add glucose and other substances, and make up water to 1000mL after melting. Sterilize at 121°C for 15 minutes.

[0040] Recovery and expanded culture of Trichoderma viride: Use a sterile pipette to draw 0.5mL of suitable liquid medium, drop it into the ampoule containing Trichoderma viride, shake gently, so that the freeze-dried strain dissolves into a suspension, and draw 100μL The bacterial suspension was transplanted on the slant medium, and cultured at the corresponding temperature of 28°C. Prepare 100 mL of comprehensive potato liquid culture medium, insert the activated strains into the culture medium on a sterile operating table, and carry out expansion culture in a constant t...

Embodiment 2

[0043]Medicinal material fermentation and extraction methods are the same as in Example 1, and the raw powder of unfermented dried Pueraria lobata medicinal material is used as a control (without adding Trichoderma viride suspension, other processing procedures are the same as in Example 1). The content of puerarin in puerarin medicinal powder before and after fermentation was detected by high performance liquid chromatography. The content of puerarin was 14.0mg / g before fermentation and 35.1mg / g after fermentation, which was the content before fermentation. 2.5 times. See Table 1 for specific data. Puerarin powder was fermented by Trichoderma viride, and the puerarin content was measured at different time points, and the average value was obtained from five parallel operations. The results showed that Trichoderma viride had an obvious increasing effect on puerarin, and reached the peak on the seventh day. After 3-5 days of fermentation, the extraction rate of the extracted ...

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Abstract

The invention provides a preparation method of puerarin extract. Firstly, trichoderma viride is used to carry out solid fermentation of kudzu root medicinal material, and then more than 90% ethanol or methanol is used to extract the fermented material, so as to obtain kudzu root with a puerarin content of more than 45% Vegetarian extract. The preparation method greatly improves the extraction yield of the puerarin, greatly reduces the production cost of the puerarin, and has a simple preparation process and is easy to realize industrialization.

Description

technical field [0001] The invention relates to a preparation method of puerarin extract, which belongs to the field of traditional Chinese medicine extraction. Background technique [0002] Pueraria lobata (Willd.) Ohwi is the root of Pueraria lobata (Willd.) Ohwi. Pueraria genus is distributed in all provinces except Xinjiang, Tibet and Qinghai in my country. Pueraria lobata contains different chemical components from roots, vines, flowers to leaves. In addition to high-quality starch, essential amino acids (lysine, methionine, phenylalanine, isoleucine, leucine, etc.) and trace elements (selenium, zinc, manganese, germanium, etc.), kudzu root also contains quantitative The main active ingredients are daidzein, daidziun, puerarin, puerarin-7-xyloside and other substances. [0003] Studies have shown that puerarin has the effects of lowering blood pressure, slowing down heart rate, dilating coronary vessels, improving microcirculation, inhibiting arteriosclerosis, anti-a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D407/04
CPCC07D407/04
Inventor 吴宪君谢相林付书清
Owner 绵阳膳意生物科技有限公司
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