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A kind of mildew-resistant bacillus strain and its application in grain storage

A technology of Bacillus spp. and Bacillus licheniformis is applied in the application, preservation of food ingredients as antimicrobials, bacteria, etc., to achieve the effect of good application prospects, strong development potential, and guaranteeing the safety of stored grains

Active Publication Date: 2020-05-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, there is no relevant report on the application of Bacillus volatile metabolites to the field of anti-mold and fresh-keeping of grain

Method used

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  • A kind of mildew-resistant bacillus strain and its application in grain storage
  • A kind of mildew-resistant bacillus strain and its application in grain storage
  • A kind of mildew-resistant bacillus strain and its application in grain storage

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: Determination of Bacillus strains of the present invention and their volatile metabolites on Aspergillus flavus antibacterial effect and comparison with other Bacillus strains

[0033] 1. Experimental method

[0034]A multi-area partitioned petri dish was selected, and potato dextrose agar medium (PDA) was added to one side of the partition for cultivating Aspergillus flavus; LB medium was added to the other side for cultivating Bacillus. In the treatment group, the activated bacillus was picked and spread on the petri dish, and the coating area was kept consistent; in the blank control group, the above operation was not performed, that is, the plate medium in this area was not inoculated. Then pick a small amount of Aspergillus flavus spores with a bamboo stick and inoculate them in the center of the PDA medium side area of ​​the petri dish in a spot-coating manner.

[0035] After the inoculation was completed and the double-layer sealing film was sealed...

Embodiment 2

[0044] Embodiment 2: the optimal culture medium screening of bacillus strain described in the present invention

[0045] 1. Experimental method

[0046] In the experiment, a multi-region partition culture dish was selected, wherein one side of the partition was added with potato dextrose agar medium (PDA) for cultivating Aspergillus flavus; In the treatment group, the activated bacillus was picked and spread on the petri dish, covering the entire area on one side of the partition of the petri dish; the blank control group did not perform the above operations, that is, they did not inoculate on the half plate. Then pick a small amount of Aspergillus flavus spores and inoculate them in the center of the area on one side of the PDA culture medium in the petri dish.

[0047] The culture medium to be screened for cultivating the bacillus described in the present invention in the experiment is NB medium, TSA medium, TSB-YE medium three kinds.

[0048] NB medium formula: 10g pepton...

Embodiment 3

[0055] Embodiment 3: simulated rice storage test

[0056] 1. Pretreatment of rice for in vivo experiments

[0057] 1.1 Experimental materials, reagents and instruments

[0058] Experimental materials: Northeast rice (purchased from a local supermarket), Aspergillus flavus, Bacillus numbered BYJ7, normal saline, sterile water, centrifuge tubes, bamboo sticks, plastic boxes, etc.

[0059] Experimental reagents: 0.3% sodium hypochlorite solution, 0.1% sodium hypochlorite solution, TSA liquid medium.

[0060] Experimental equipment: VOCs test bottle, shaker, optical microscope, oven, ultra-clean bench, etc.

[0061] 1.2 Experimental method

[0062] (1) Construction of VOCs test bottle device

[0063] Two 250mL upper nozzle filter bottles and a two-way straight plunger are connected through rubber tubes. A 250mL filter bottle with an upper mouth was sealed with a rubber stopper (the bottle was used for culturing Bacillus), and the other 250mL filter bottle with an upper mouth ...

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Abstract

The invention discloses a mildew-resistant bacillus strain and its screening and application. The strain is classified as Bacillus licheniformis BYJ7, and is preserved in the China General Microorganism Culture Collection and Management Center with the preservation number CGMCC 16705. The invention also provides a method for preparing the bacillus culture solution and its volatile metabolites. The Bacillus licheniformis and its volatile metabolites in the present invention can well inhibit the growth of main spoilage bacteria in stored grains (especially rice), and have a wide application range, good application prospects and strong development potential; And it has the characteristics of high efficiency, energy saving, environment friendliness, etc., and can be applied to the preparation of grain antifungal agent.

Description

technical field [0001] The invention belongs to the field of new strain screening technology and microorganism technology, and in particular relates to an anti-mold Bacillus strain and its anti-mold application in grain storage. Background technique [0002] Rice is one of the main food crops in southern my country. However, due to the influence of individual farmers' planting and storage methods, as well as the high temperature and high humidity weather in the Yangtze River Basin and South China, rice is contaminated by mycotoxins during storage, transportation and sales. In severe cases, it is extremely prone to mildew. [0003] At home and abroad, the methods of rice preservation are mainly studied from the following aspects: one is to study the mechanism of rice aging, to control aging to improve the storage period of rice, and to delay the storage of water, protein, starch and fat in rice. The second is to kill the adults, eggs, and bacteria attached to the rice or dest...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02A23B9/28C12R1/10
CPCA23B9/28A23V2002/00C12N1/02C12N1/20C12N1/205C12R2001/10A23V2200/10Y02P60/85
Inventor 周文文张一鞠刘姝妤汤曦
Owner ZHEJIANG UNIV
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