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Method for preparing carvacrol microcapsule antibacterial agent from attapulgite stabilized oil-in-water emulsion

A technology of oil-in-water emulsion and attapulgite, which is applied in microcapsule preparations, botany equipment and methods, chemicals for biological control, etc., can solve irritating odor, poor thermal stability of carvacrol, volatility, Limit the use efficiency and other issues, achieve high antibacterial persistence, solve long-term dependence on antibiotics, and prevent volatilization

Active Publication Date: 2019-03-08
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the use of carvacrol, carvacrol has a certain pungent odor due to its poor thermal stability and volatility, which limits its use efficiency in animal breeding.

Method used

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  • Method for preparing carvacrol microcapsule antibacterial agent from attapulgite stabilized oil-in-water emulsion
  • Method for preparing carvacrol microcapsule antibacterial agent from attapulgite stabilized oil-in-water emulsion
  • Method for preparing carvacrol microcapsule antibacterial agent from attapulgite stabilized oil-in-water emulsion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Preparation of attapulgite-stabilized oil-in-water emulsion

[0048] Under the stirring condition of 200r / min, 50mL of 0.5 wt% sodium alginate solution was mixed with 30mL of 10 wt% attapulgite suspension to form a uniform suspension A;

[0049] 0.2g of Span 80 and 40g of carvacrol were fully mixed and emulsified, then added to 20mL of 20 wt% quaternized chitosan oligosaccharide solution, stirred at 150r / min for 30min to form solution B;

[0050] Add solution B to solution A, and stir and emulsify at 6000r / min for 60min to form attapulgite stable oil-in-water emulsion.

[0051] (2) Preparation of carvacrol microcapsules

[0052] Under slow stirring (30r / min), use a peristaltic pump to drop the attapulgite stable oil-in-water emulsion into 0.5 wt% calcium chloride solution, and the speed of the peristaltic pump is 3r / min; The formed microcapsules were separated, dried at 37°C, and packaged. The encapsulation efficiency of the obtained carvacrol microcapsules was 5...

Embodiment 2

[0054] (1) Preparation of attapulgite-stabilized oil-in-water emulsion

[0055] Under the stirring condition of 150r / min, 50mL of 1.0 wt% sodium alginate solution was mixed with 50mL of 15 wt% attapulgite suspension to form a uniform suspension A;

[0056] After fully mixing 0.3g of Span 80 and 30g of carvacrol, add it to 10mL of 30 wt% quaternized chitosan solution, and stir at 250r / min for 20min to form solution B;

[0057] Add B to A and emulsify at 8000r / min for 30min to form attapulgite stable oil-in-water emulsion.

[0058] (2) Preparation of carvacrol microcapsules

[0059] Under slow stirring (40r / min), use a peristaltic pump to drop the attapulgite stable oil-in-water emulsion into 1.5 wt% calcium chloride solution, and the speed of the peristaltic pump is 4r / min; The formed microcapsules were separated, dried at 37°C, and packaged. The encapsulation efficiency of the obtained carvacrol microcapsules was 62.4%, and the oil content was 47.8%.

Embodiment 3

[0061] (1) Preparation of attapulgite-stabilized oil-in-water emulsion

[0062] Under the stirring condition of 200r / min, 50mL of 1.5 wt% sodium alginate solution was mixed with 40mL of 15 wt% attapulgite suspension to form a uniform suspension A.

[0063] After fully mixing 0.15g of Span 80 and 20g of carvacrol, add it to 20mL of 15 wt% quaternized chitosan solution, stir at 300r / min for 20min to form solution B;

[0064] Add B to A and emulsify at 7000r / min for 40min to form attapulgite stable oil-in-water emulsion.

[0065] (2) Preparation of carvacrol microcapsules

[0066] Under slow stirring (50r / min), use a peristaltic pump to drop the attapulgite stable oil-in-water emulsion obtained in step 1 into 2.0 wt% calcium chloride solution, and the speed of the peristaltic pump is 5r / min; After 20 minutes, the formed microcapsules were separated, dried at 37°C, and packaged. The encapsulation efficiency of the obtained carvacrol microcapsules was 65.5%, and the oil content ...

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Abstract

The invention discloses a method for preparing a carvacrol microcapsule antibacterial agent from attapulgite stabilized oil-in-water emulsion. The method comprises the following steps: firstly, preparing the attapulgite stabilized oil-in-water emulsion from antibacterial agent carvacrol molecules under the action of an emulsifying agent on the basis of water solubility and cationic property of quaternized chitosan oligosaccharide and in combination with biological activity and nanometer effect of natural nano-material attapulgite; then, encapsulating the natural antibacterial agent carvacrol with volatile and irritating odor with a microcapsule preparation technology to prevent volatilization of carvacrol; further forming a protective shell layer by cross-linking with calcium chloride; finally, obtaining the carvacrol microcapsule antibacterial agent by separation and drying. The carvacrol microcapsule antibacterial agent has broad-spectrum antibacterial activity and high antibacterialefficiency, is free of irritating smell, also has high thermal stability and antibacterial durability, can be applied to feed additives, and solves the current situation of long-term dependence on antibiotics in the animal breeding process in China.

Description

technical field [0001] The invention relates to a method for preparing a carvacrol microcapsule antibacterial agent, in particular to a method for preparing a carvacrol microcapsule antibacterial agent by using attapulgite to stabilize an oil-in-water emulsion, and belongs to the field of clay mineral functional materials. Background technique [0002] In recent years, the phenomenon of bacterial resistance caused by the abuse of antibiotics has become increasingly serious, and antibiotic resistance is threatening human health. It is extremely urgent to find new alternatives to antibiotics and provide effective and feasible antibacterial strategies (Journal of Antimicrobial Chemotherapy, 2014, 69: 827–834). The abuse of antibiotics is particularly serious in the livestock industry. At present, livestock farmers generally add feed antibiotics to feed as additives to promote the growth of animals, which not only increases the risk of "super bacteria", but also causes serious ...

Claims

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Application Information

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IPC IPC(8): A01N31/08A01N25/28A01P1/00B01J13/14
CPCA01N25/28A01N31/08B01J13/14
Inventor 王爱勤惠爱平康玉茹朱永峰汪琴
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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