Streptomycete and application thereof

A technology of Streptomyces and Streptomyces regis, applied in the application, bacteria, biocide and other directions, can solve the problems of poor stability and low insecticidal efficiency of antibiotics, and achieve high insecticidal activity, wide insecticidal spectrum, and genetic stability. Good results

Active Publication Date: 2019-03-08
JILIN CITY ACADEMY OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing antibiotics generally have problems such as low insecticidal efficiency and poor stability.

Method used

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  • Streptomycete and application thereof
  • Streptomycete and application thereof
  • Streptomycete and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 1.2.1 Soil sample collection

[0055] After selecting a suitable location, use a small shovel to remove the topsoil, take about 30-50g of soil at a depth of 5-10cm, put it into a sterilized paper bag, number it, and indicate the collection location and date for the isolation of Streptomyces .

[0056] 1.2.2 Isolation and purification of Streptomyces

[0057] Streptomyces was isolated and purified by plate dilution separation method (Reference: Fang Zhongda. Plant Disease Research Methods [M] Beijing: China Agricultural Press, 2007, 243-250). In order to reduce the contamination of miscellaneous bacteria, especially bacteria, first bake 10g of the weighed soil at 120°C for 1h, then add 100mL of sterile water, shake for 30min, draw 1mL of the suspension, and dilute it with sterile water gradient, from 10 -3 、10 -4 and 10 -5 Pipette 0.1mL from the dilution tubes and add them to the Gaoshi No. 1 plate, spread evenly, and then place the culture dish upside down in a 28°...

Embodiment 2

[0068] 1.2.5 Determination of broad-spectrum insecticidal activity of Streptomyces LKY208 fermentation broth

[0069] Determination of insecticidal activity against calico moth, American white moth, diamondback moth, cabbage caterpillar and cabbage armyworm: use the leaf dipping method (the method is the same as 1.2.4).

[0070] Determination of insecticidal activity against corn borer: using feed poisoning method (references: Xu Shulan, Li Hui, Chen Qijin. Pathogenicity determination of beet armyworm nuclear polyhedrosis virus and the effect of group breeding on virus yield[J]. Changjiang Vegetables, 2010(18):10-12.). Evenly cut feed of a certain size (approximately the daily food intake of larvae), mix the fermentation liquid of the strain into the artificial feed after autoclaving (100 μ L / g), and feed the 2nd instar corn borer larvae, 20 larvae per treatment, Repeat 3 times. The rearing conditions were the same as above. After 48 hours, the survival or death of the test ...

Embodiment 3

[0075] 1.2.6 Determination of thermal stability

[0076] Take the same amount of fermentation liquid of the strain and treat it at 30°C, 40°C, 50°C, 60°C, 70°C, 80°C, 90°C and 100°C for 1 hour, take it out, cool it naturally, and use the method of soaking leaves to treat the 2-3 year old The insecticidal activity was detected on the larvae of C. chinensis, and the untreated fermentation broth (25°C) was used as the control.

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Abstract

The invention relates to streptomycete and application thereof. The strain is Streptomyces regensis and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 16746. The streptomycete can be used in the field of biological prevention and control and is used for preventing and controlling camptoloma interiorata, hyphantria cunea, plutella xylostella, ostriniafurnacalis, tetranychus urticae, cabbage caterpillar, mamestra brassicae, henosepilachna vigintioctomaculata, myzus persicae and dolycoris baccarum Linnaeus. The streptomycete has the characteristicsof high insecticidal activity, wide insecticidal spectrum, good genetic stability and the like; a strain fermentation solution has the advantages of strong temperature tolerance, stable insecticidalstability, good light stability, storage resistance and the like, and has very good development and application values.

Description

technical field [0001] The invention belongs to the technical field of biological control, and relates to a strain of streptomyces and its application. Background technique [0002] Actinomycetes are the first type of microorganisms found to have biocontrol effects. It has been reported that a variety of streptomyces in actinomycetes can produce new insect-resistant antibiotics. The third milestone of application in production is the most potential antibiotic in agricultural production. Among the more than 3,000 new antibiotics reported in recent years, insecticidal antibiotics account for about 5%. Some antibiotic varieties with development potential have been discovered. However, the existing antibiotics generally have problems such as low insecticidal efficiency and poor stability. Therefore, the development of a highly efficient and stable new Streptomyces or its biocontrol products is crucial to the sustainable development of agriculture and forestry. Contents of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P7/04C12R1/465
CPCA01N63/28
Inventor 李立梅陈越渠宋丽文李兴鹏左彤彤邹建军毛赫勾天兵杨帆陈思羽刘庆珍
Owner JILIN CITY ACADEMY OF FORESTRY
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