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Application of gene ESP for regulating and controlling development of rice spikes

A rice and gene technology, applied in the genetic improvement of rice panicle type, the application of gene ESP, the field of gene ESP to control rice panicle type, can solve the problems of limited understanding of molecular regulation mechanism, and improve the population structure and light receiving situation , reduce shading, broaden the effect of genetic basis

Active Publication Date: 2019-03-08
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current understanding of the molecular regulation mechanism of rice panicle formation is still very limited.

Method used

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  • Application of gene ESP for regulating and controlling development of rice spikes
  • Application of gene ESP for regulating and controlling development of rice spikes
  • Application of gene ESP for regulating and controlling development of rice spikes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 rice esp mutant phenotype and genetic effect analysis

[0039] Among the progeny of wild-type japonica rice Zhonghua 11, a plant with slightly shorter panicle length, erect panicle type and increased grain density was screened out, which was named rice esp mutant.

[0040] At the grain filling stage and maturity stage, the plants and ear types of the wild-type and mutant heterozygotes were photographed, and the phenotypes were as follows: figure 1 As shown in A and 1B, the spikes of the esp heterozygous mutants were shorter than those of the wild type, and the grain density increased ( figure 1 A and 1B). Interestingly, this mutation has a low-frequency spontaneous back mutation phenomenon, so different tillers of a single plant or even different panicle branches of the same rice panicle can show the coexistence of wild type and mutant type. The seedling phenotypes of esp homozygous mutants and wild type are as follows figure 1 As shown in C, the esp hom...

Embodiment 2e

[0042] Embodiment 2Esp mutant panicle character analysis

[0043] The spikes of esp heterozygous mutants and wild-type rice from the completion of grouting to yellow maturity were photographed, and statistical analysis was performed on the panicle traits such as spike length, grain shape, number of primary branches, and primary branch length. The results are as follows figure 2 shown. The panicle length of esp heterozygous mutants was significantly shorter ( figure 2 A and 2B), the primary branch length was also significantly shortened ( figure 2 C), while the grain shape and the number of primary branches did not change significantly ( figure 2 A and 2D), indicating that ESP mainly affects the panicle shape and panicle length by affecting the primary branch development of rice panicle, and basically has no effect on the number of primary branches and grain shape.

Embodiment 3

[0044] Map-position cloning and candidate gene expression analysis of embodiment 3ESP gene

[0045] F constructed using the esp mutant and the indica rice variety Huajingxian 74 (a rice variety publicly available, commercially available) 2 population (esp crossed with Huajingxian 74 to obtain F 1 , F 1 F 2 Population), ESP gene mapping was carried out, and it was initially located between the SSR markers RM583 and RM9 on chromosome 1, with a genetic distance of 42.4cM. According to the Nipponbare and 9311 reference sequences, an Indel (insertion-deletion) marker was developed between these two markers, and the ESP gene was further positioned on the 51.2kb interval of the rice chromosome 1 ( image 3 A). There are 4 genes in this interval, namely Os01g0356900, Os01g0356951, Os01g0357100 and Os01g0357200.

[0046] In order to identify the candidate gene ESP, the expression levels of these four genes in the esp mutant and wild type were detected respectively, and the specifi...

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Abstract

The invention discloses application of a gene ESP for regulating and controlling development of rice spikes and belongs to the field of plant genetic engineering. According to the invention, the geneESP for regulating and controlling development of the rice spikes is obtained by adopting a map-based cloning method for cloning; the gene ESP has a DNA sequence shown in SEQ ID NO:1; and the gene ESPis proved to play an important role in regulating and controlling the type of the rice spikes by mutant phenotypic analysis and complementary analysis. The cloning of the gene ESP not only enriches the molecular mechanism for regulating and controlling the type of the rice spikes and has an important theoretical significance in revealing the development mechanism of the rice spikes, but also provides a new clue for researching the evolution of the type of the rice spikes. Meanwhile, a new gene resource is provided for genetic improvement of the type of the rice spikes and a new material choice is provided for rice molecular design breeding. A rice variety with vertical dense spikes can be cultivated by increasing the expression of ESP in rice, and the operability is ultrahigh, so that a new approach for widening the genetic basis of the existing vertical spike variety is opened up.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and in particular relates to the application of a gene ESP that regulates rice panicle development, and specifically relates to the use of a map-based cloning strategy to isolate and clone a gene ESP that controls rice panicle type, the biological function verification of the gene, and the genetic Transformation and its application as a gene regulating panicle development in the genetic improvement of rice panicle shape. Background technique [0002] Rice is an important food crop in my country and even in the world, and more than half of the world's population uses rice as a staple food. The panicle length and grain density of rice are important factors affecting the yield. Therefore, elucidating the genetic basis and molecular regulation mechanism of panicle development will help improve the panicle shape and yield of rice. [0003] Rice panicle type is of great significance in the stu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H1/02A01H6/46
CPCA01H1/02C07K14/415C12N15/8261
Inventor 张向前栾鑫彭海峰柯善文刘树春代航
Owner SOUTH CHINA AGRI UNIV
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