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Composite microbial inoculum for improving tobacco quality

A compound microbial agent and compound bacterial agent technology, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., to achieve the effects of short treatment cycle, low treatment cost, and improved quality of tobacco leaves

Inactive Publication Date: 2019-03-19
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practical applications, due to the different metabolites of different microbial strains and the needs of different processing techniques, it is often necessary to use different strains and strains in combination. However, due to different growth characteristics of different strains and strains, therefore Further research and discussion are needed on whether it can be compounded and the application effect after compounding

Method used

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  • Composite microbial inoculum for improving tobacco quality
  • Composite microbial inoculum for improving tobacco quality
  • Composite microbial inoculum for improving tobacco quality

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The composite microbial bacterial agent provided in this application is specifically prepared through the following steps.

[0043] (1) Preparation of Bacillus thuringiensis XC-3 active bacterial agent

[0044] Place the Bacillus thuringiensis XC-3 strain on the LB plate, activate and cultivate it at 37°C for 72 hours, collect the cultured colonies, inoculate them in liquid LB medium, and culture them overnight on a shaker at 37°C as the seed solution;

[0045] The seed solution was transferred to liquid LB medium at a volume ratio of 1%, and cultivated to OD at 30°C and 150 rpm. 600 =2.0 or so as fermentation broth;

[0046] Centrifuge the fermentation broth at 3600 rpm for 10min, discard the supernatant, then add sterile water to suspend fully, and adjust the number of viable bacteria to (10~11)×10 7 A / mL spare.

[0047] (2) Preparation of Bacillus pumilus SMXP-3 active bacterial agent

[0048] Put the Bacillus pumilus SMXP-3 strain on the LB plate, and after 72 h...

Embodiment 2

[0058] When using the composite microbial bacterial agent prepared in Example 1 to treat tobacco leaves, in order to determine the optimal ratio of the composite microbial bacterial agent and its specific effect on tobacco leaves, the inventors carried out partial orthogonal experiment optimization. The specific process is:

[0059] According to the single dosage ratio of each active bacterial agent in the tobacco leaves, the three kinds of bacterial solutions were absorbed and mixed, and then the mixed bacterial solutions were evenly applied to the tobacco leaves (each treated tobacco leaf was 100 g). The single-bacteria solution treatment group, and then adjust the moisture content of the tobacco leaves of each experimental group to 25%, place them in a constant temperature and humidity box (control temperature at 37°C, humidity at 75%), take them out after 72 hours of fermentation, and place them in an oven at 80°C for 20 min, to inactivate the microorganisms, and at the sam...

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Abstract

The invention belongs to the technical field of cigarette raw material production processing, and particularly relates to the patent application issue of a composite microbial inoculum for improving tobacco quality prepared by using specific microorganism strains. The effective ingredients of the composite microbial inoculum are the viable bacteria of a bacillus thuringiensis strain XC-3, a bacillus pumilus strain SMXP-3 and a bacillus subtilis strain SMXP-58. The microbial inoculum is the combination which is obtained after liquid culturing objects are mixed. According to microbial inoculum,a simpler technical scheme of using a composite microbial inoculum preparation for processing reconstituted tobaccos is designed through the screening of the specific strains and the combination developing of the composite microbial preparation, so that the microbial inoculum has the advantages that the technological process is simpler, the processing cost is lower, the processing period is shorter, the content of starch, protein and pectin in the tobaccos can be significantly reduced, the quality of the tobaccos is improved, and therefore the microbial inoculum has better application popularization value in the technical field of cigarette production.

Description

technical field [0001] The invention belongs to the technical field of production and processing of cigarette raw materials, and specifically relates to a patent application for a compound microbial bacterial agent prepared by using a specific microbial strain for improving the quality of tobacco leaves. Background technique [0002] Tobacco is one of the important economic crops in our country and occupies an important position in the national economy. Tobacco leaves are the basis for the development of the tobacco industry. Continuously improving the quality of tobacco leaves is one of the important goals of tobacco leaf production. "Improving quality and optimizing structure" has always been the focus of tobacco leaf work. [0003] Pectin, starch, protein, etc. are the main macromolecular chemical components in tobacco leaves. Among them, pectin is the main substance that constitutes the plant cell wall, which has a direct impact on the combustibility of tobacco products...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/20A24B3/12C12N1/20C12R1/125C12R1/07
CPCA24B3/12A24B15/20C12N1/20
Inventor 杨宗灿张展刘超程良琨张婷婷冯颖杰刘向真许衡彭玉富申洪涛王根发
Owner CHINA TOBACCO HENAN IND
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