Composite microbial inoculum for improving tobacco quality
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A compound microbial agent and compound bacterial agent technology, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., to achieve the effects of short treatment cycle, low treatment cost, and improved quality of tobacco leaves
Inactive Publication Date: 2019-03-19
CHINA TOBACCO HENAN IND
View PDF7 Cites 2 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, in practical applications, due to the different metabolites of different microbial strains and the needs of different processing techniques, it is often necessary to use different strains and strains in combination. However, due to different growth characteristics of different strains and strains, therefore Further research and discussion are needed on whether it can be compounded and the application effect after compounding
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0042] The composite microbial bacterial agent provided in this application is specifically prepared through the following steps.
[0043] (1) Preparation of Bacillus thuringiensis XC-3 active bacterial agent
[0044] Place the Bacillus thuringiensis XC-3 strain on the LB plate, activate and cultivate it at 37°C for 72 hours, collect the cultured colonies, inoculate them in liquid LB medium, and culture them overnight on a shaker at 37°C as the seed solution;
[0045] The seed solution was transferred to liquid LB medium at a volume ratio of 1%, and cultivated to OD at 30°C and 150 rpm. 600 =2.0 or so as fermentation broth;
[0046] Centrifuge the fermentation broth at 3600 rpm for 10min, discard the supernatant, then add sterile water to suspend fully, and adjust the number of viable bacteria to (10~11)×10 7 A / mL spare.
[0047] (2) Preparation of Bacillus pumilus SMXP-3 active bacterial agent
[0048] Put the Bacillus pumilus SMXP-3 strain on the LB plate, and after 72 h...
Embodiment 2
[0058] When using the composite microbial bacterial agent prepared in Example 1 to treat tobacco leaves, in order to determine the optimal ratio of the composite microbial bacterial agent and its specific effect on tobacco leaves, the inventors carried out partial orthogonal experiment optimization. The specific process is:
[0059] According to the single dosage ratio of each active bacterial agent in the tobacco leaves, the three kinds of bacterial solutions were absorbed and mixed, and then the mixed bacterial solutions were evenly applied to the tobacco leaves (each treated tobacco leaf was 100 g). The single-bacteria solution treatment group, and then adjust the moisture content of the tobacco leaves of each experimental group to 25%, place them in a constant temperature and humidity box (control temperature at 37°C, humidity at 75%), take them out after 72 hours of fermentation, and place them in an oven at 80°C for 20 min, to inactivate the microorganisms, and at the sam...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention belongs to the technical field of cigarette raw material production processing, and particularly relates to the patent application issue of a composite microbial inoculum for improving tobacco quality prepared by using specific microorganism strains. The effective ingredients of the composite microbial inoculum are the viable bacteria of a bacillus thuringiensis strain XC-3, a bacillus pumilus strain SMXP-3 and a bacillus subtilis strain SMXP-58. The microbial inoculum is the combination which is obtained after liquid culturing objects are mixed. According to microbial inoculum,a simpler technical scheme of using a composite microbial inoculum preparation for processing reconstituted tobaccos is designed through the screening of the specific strains and the combination developing of the composite microbial preparation, so that the microbial inoculum has the advantages that the technological process is simpler, the processing cost is lower, the processing period is shorter, the content of starch, protein and pectin in the tobaccos can be significantly reduced, the quality of the tobaccos is improved, and therefore the microbial inoculum has better application popularization value in the technical field of cigarette production.
Description
technical field [0001] The invention belongs to the technical field of production and processing of cigarette raw materials, and specifically relates to a patent application for a compound microbial bacterial agent prepared by using a specific microbial strain for improving the quality of tobacco leaves. Background technique [0002] Tobacco is one of the important economic crops in our country and occupies an important position in the national economy. Tobacco leaves are the basis for the development of the tobacco industry. Continuously improving the quality of tobacco leaves is one of the important goals of tobacco leaf production. "Improving quality and optimizing structure" has always been the focus of tobacco leaf work. [0003] Pectin, starch, protein, etc. are the main macromolecular chemical components in tobacco leaves. Among them, pectin is the main substance that constitutes the plant cell wall, which has a direct impact on the combustibility of tobacco products...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more