Unlock instant, AI-driven research and patent intelligence for your innovation.

A key gene mutant for alkanes originating from cyanobacteria and its application

A key technology for alkane production, applied in applications, genetic engineering, plant genetic improvement, etc., can solve the problems of difficulty in meeting industrialization needs and low biological alkane production, and achieve speeding up the commercialization process, improving productivity, and reducing costs. Effect

Active Publication Date: 2022-02-11
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to overcome the deficiencies of the prior art, to solve the existing technical problems of low bio-alkane production based on wild-type hydrocarbon-producing genes, and difficulty in meeting the needs of industrialization

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A key gene mutant for alkanes originating from cyanobacteria and its application
  • A key gene mutant for alkanes originating from cyanobacteria and its application
  • A key gene mutant for alkanes originating from cyanobacteria and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Test material

[0032] 1) Preparation of LB medium:

[0033] LB liquid medium: 10g / L tryptone, 5g / L yeast extract, 10g / L sodium chloride;

[0034] LB solid medium: add 15g of agar per liter of LB liquid medium;

[0035] Kanamycin-resistant LB solid medium: Heat the prepared LB solid medium to dissolve completely, add a total of 2‰ (v / v) of kanamycin when the temperature drops to about 55°C, and then slowly Pour into a petri dish, cool and solidify for later use.

[0036] 2) Improved M9 buffer: take 6g of Na 2 HPO 4 , 3g of KH 2 PO 4 , 0.5g of Nacl was dissolved and fixed in 1L of ultrapure water, and sterilized at high temperature and high pressure to obtain liquid A. Weigh the rest of the components and sterilize them separately under high temperature and high pressure or by filtration, and add the following components to liquid A: 2g / L NH 4 Cl, 0.25g / L MgSO 4 ⅹ7H 2 O, 11mg / L of CaCl 2 , 27mg / L of FeCl 3 ⅹ6H 2 O, 2mg / L ZnClⅹ4H 2 O, 2mg / L Na 2 MoO 4 ⅹ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a mutant of alkane-producing key gene derived from cyanobacteria, the gene has the nucleotide sequence shown in SEQ ID NO: 1, or is complementary to the nucleotide sequence shown in SEQ ID NO: 1 the nucleotide sequence. The invention also discloses a method and application of the plasmid pEASY-1594-1711 constructed from the wild-type alkanes-producing key gene derived from cyanobacteria as a template to obtain the key hydrocarbon-producing gene mutant. The hydrocarbon-producing gene is a mutant of the wild-type hydrocarbon-producing gene derived from cyanobacteria. The total amount of biological alkanes produced by the strain based on the hydrocarbon-producing gene mutant is 2.9 times higher than that of the wild type, thereby improving the biological production of alkanes. Reducing the cost of bio-oil production will help accelerate the commercialization of bio-oil production.

Description

technical field [0001] The invention relates to the bioenergy field of genetic engineering, in particular to a mutant of a key gene for alkanes originating from cyanobacteria and its application. Background technique [0002] With the rapid growth of the global economy and the sharp increase in energy demand, fossil fuel resources such as petroleum continue to decrease, and the environmental problems caused by the burning of fossil fuels are increasing day by day, the development of renewable biofuels has gradually attracted people's attention (G.Stephanopoulos, Challenges in enginerting microbes forbiofuels production, Science 315(2007) 801-804). As a renewable energy source, further research on bioenergy can ease or even eliminate the energy crisis. [0003] Alkanes are the most important components of gasoline, diesel, and aviation kerosene. Bio-produced alkanes are closer to the existing fossil-sourced diesel and are ideal substitutes for diesel. Hydrocarbons exist wid...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/31C12N15/70C12N1/21C07K14/195C12P5/00C12R1/19
CPCC12N15/70C12P5/00C07K14/195
Inventor 李鹿之陈少鹏吴李君
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI