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Application of traditional Chinese medicine sulfated radix codonopsis polysaccharide as antioxidant

A technology of Codonopsis polysaccharide and antioxidant, applied in the field of animal antioxidant drugs, can solve the problems of complex ingredients, weak antioxidant effect of traditional Chinese medicine, and difficult quality control.

Inactive Publication Date: 2019-03-22
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] A large number of studies have shown that many traditional Chinese medicines and their active ingredients have certain antioxidant effects. However, due to the complexity of the ingredients and the difficulty in quality control, the antioxidative effects of traditional Chinese medicines are weak, and the effect is not ideal.
However, there is currently no information on the use of sulfated Codonopsis polysaccharides for inhibiting and / or repairing H 2 o 2 Oxidative damage has been reported

Method used

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  • Application of traditional Chinese medicine sulfated radix codonopsis polysaccharide as antioxidant
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  • Application of traditional Chinese medicine sulfated radix codonopsis polysaccharide as antioxidant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation process of sulfated Codonopsis polysaccharide is as follows:

[0026] The total polysaccharides of Codonopsis pilosula were extracted from Codonopsis pilosula decoction pieces by water decoction and alcohol precipitation method, and then the protein in the polysaccharides was removed by sevage method, purified by DEAE-52 cellulose column, and freeze-dried to obtain purified polysaccharides. Sulfated Codonopsis polysaccharides were modified by chlorosulfonic acid-pyridine method, and the modification process conditions were optimized. The modified polysaccharides were purified by dialysis and freeze-dried to obtain sulfated Codonopsis polysaccharides. The phenol-sulfuric acid method and ultrasonic acid chromic acid The barium method was used to determine that the polysaccharide mass proportion of the prepared Codonopsis polysaccharide was 66.30%, and the sulfate radical mass proportion was 34.48%.

Embodiment 2

[0028] 1. Test method

[0029] Test cells: macrophages.

[0030] Adjust the concentration of cells in the logarithmic growth phase to 5 × 10 4 Each sample / mL was added to a 96-well plate, 100 μL per well, and after 24 hours of adherent culture, 100 μL of different concentrations of Codonopsis polysaccharides (Dangshen polysaccharide treatment group) and sulfated Codonopsis polysaccharides (sulfated Codonopsis polysaccharide treatment group) were added, and a blank was set The control group, model control group and positive control group (VC control group) were cultured for 24 h, and then each group was added with a final concentration of 12.5 mmol / L H 2 o 2 Incubate for 1 h, add 30 μL of MTT and continue to culture for 4 h, remove the supernatant, add 100 mL of DMSO, shake well, and detect with ELISA A 570 The value is used to screen the safe concentration range of drugs for macrophages.

[0031] The blank control group was DMEM culture medium supplemented with 10% fetal ...

Embodiment 3

[0034] With the concentration range that embodiment 2 tests further research sulfated Codonopsis polysaccharide is for in H 2 o 2 The growth status of macrophages under stimulation was observed.

[0035] testing method:

[0036] Adjust the concentration of cells in the logarithmic growth phase to 5 × 10 4 Each sample / mL was added to a 96-well plate, 100 μL per well, and after 24 hours of adherent culture, different concentrations of sulfated Codonopsis polysaccharides were added (sulfated Codonopsis polysaccharide treatment group), and a blank control group, a model control group, a Codonopsis polysaccharide control group, Positive control group (VC control group), after continuing to culture for 24 h, add H at a final concentration of 12.5 mmol / L 2 o 2 Cultured for 1 h, placed under an inverted microscope, and observed the growth status of the cells at 250× and 400× magnifications, respectively.

[0037] The blank control group was DMEM culture medium supplemented with 1...

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Abstract

The invention discloses application of a traditional Chinese medicine sulfated radix codonopsis polysaccharide as an antioxidant. Through research, the sulfated radix codonopsis polysaccharide is found to be capable of ensuring the growth morphology of macrophages stimulated by H2O2 and no significant differences existing between detection indexes and normal conditions, especially that the contents of malondialdehyde, superoxide dismutase and glutathione peroxidase in the macrophages are at normal levels, and the traditional Chinese medicine sulfated radix codonopsis polysaccharide has a goodpreventive and / or therapeutic effect on stress oxidative damage in mammals induced by H2O2 stimulation, and is of great value in veterinary clinical application.

Description

technical field [0001] The invention relates to the technical field of animal antioxidant drugs, more specifically, the application of the traditional Chinese medicine sulfated Codonopsis polysaccharide as an antioxidant. Background technique [0002] Oxidative stress refers to that when the body is subjected to various harmful stimuli, the body produces too many high-activity molecular free radicals, the degree of oxidation exceeds the ability to scavenge oxides, and the dynamic imbalance between the oxidation system and the antioxidant system leads to tissue damage. In the animal husbandry production chain, oxidative stress caused by factors such as intensive breeding, malnutrition, feed fermentation, long-distance transportation and extreme environments affects the development of the breeding industry and causes huge economic losses. In order to solve the production problems caused by oxidative stress, research on exogenous antioxidants has been extensively carried out. ...

Claims

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Application Information

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IPC IPC(8): A61K31/737A61P39/06
CPCA61K31/737A61P39/06
Inventor 刘翠孙嘉琪谭盈
Owner SOUTH CHINA AGRI UNIV
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