Phenylbenzofuran compound, preparation method thereof, composition and medical application
A compound, phenylbenzene technology, applied in the field of medicine, can solve the problems of increasing cancer burden and shortage of drugs for nasopharyngeal cancer, and achieve the effects of low production cost, easy purification, and mild reaction conditions
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Embodiment 1
[0061] Chinese medicine extraction method of phenylbenzofuran compound
[0062] The specific process of Chinese medicine extraction method:
[0063] Step 1, extracting the pea root coarse powder with 75% ethanol solution in parts by mass (specifically, multiple extractions can be carried out, and each extraction is separated by one week) to obtain the extract, and the ethanol solution is removed under reduced pressure, and the extract is concentrated to obtain Concentrate, the mass ratio of bean root meal to ethanol solution is 1:3, and the amount of bean root meal is 55kg;
[0064] Step 2, adjusting the pH of the concentrate to 2 with a hydrochloric acid solution with a concentration of 0.4M, and extracting with ethyl acetate, collecting the organic phase, removing the ethyl acetate and concentrating to obtain an extract;
[0065] Step 3. First, purify the extract by silica gel column chromatography. The elution system is a chloroform-methanol system, and collect the eluate...
Embodiment 2
[0066] Chinese medicine extraction method of phenylbenzofuran compound
[0067] The specific process of Chinese medicine extraction method:
[0068] Step 1, extracting the pea root coarse powder with 75% ethanol solution in parts by mass (specifically, multiple extractions can be carried out, and each extraction is separated by one week) to obtain the extract, and the ethanol solution is removed under reduced pressure, and the extract is concentrated to obtain Concentrate, the mass ratio of bean root meal to ethanol solution is 1:4, and the amount of bean root meal is 55kg;
[0069] Step 2, adjusting the pH of the concentrate to 3 with a hydrochloric acid solution with a concentration of 0.4M, and extracting it with ethyl acetate, collecting the organic phase, removing the ethyl acetate and concentrating to obtain an extract;
[0070] Step 3. First, purify the extract by silica gel column chromatography, the elution system is a chloroform-methanol system, and collect the elu...
Embodiment 3
[0071] Chinese medicine extraction method of phenylbenzofuran compound
[0072] Step 1, extracting the pea root coarse powder with 75% ethanol solution in parts by mass (specifically, multiple extractions can be carried out, and each extraction is separated by one week) to obtain the extract, and the ethanol solution is removed under reduced pressure, and the extract is concentrated to obtain Concentrate, the mass ratio of bean root meal and ethanol solution is 1:5, and the amount of bean root meal is 55kg;
[0073] Step 2, adjusting the pH of the concentrate to 4 with a hydrochloric acid solution with a concentration of 0.4M, and extracting with ethyl acetate, collecting the organic phase, removing the ethyl acetate and concentrating to obtain an extract;
[0074] Step 3. First, purify the extract by silica gel column chromatography. The elution system is a chloroform-methanol system, and collect the eluate with a volume ratio of chloroform-methanol of 20:1 to obtain the fir...
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