Production process of heparin sodium in porcine small intestine mucosa

Abstract

The invention discloses a production process of heparin sodium in porcine small intestine mucosa. The production process comprises the following steps that the intestinal mucosa is added into an enzymatic hydrolysis tank, stirring is performed for 10 minutes by adding salt and then heating is performed to reach the temperature of 40-45 DEG C, heating is stopped and stirring is performed for 10 minutes, the pH value is adjusted to 7.2-7.5 with a sodium hydroxide solution, 2709 protease is added after stirring is performed for another 10 minutes, stirring is performed for another 10 minutes andthen heating is performed to 56-58 DEG C, stirring is continued to be performed for 30 minutes and stirring is stopped and heat preservation is performed for 2 hours, temperature is raised to 85 DEG Cafter quick stirring after the heat preservation is performed for 2 hours, then heating is slowly performed to 90-92 DEG C after the temperature is raised to 85 DEG C, heat preservation is performedfor 30-60 minutes, the liquid in the enzymatic hydrolysis tank is layered, the upper layer is protein, the lower layer is a clear enzymatic hydrolysate, the protein and the enzymatic hydrolysate are separated, and the enzymatic hydrolysate is subjected to adsorption, washing, elution, precipitation and drying to obtain the crude heparin sodium. The problems that the content of protein contained inthe enzymatic hydrolysate after the enzymatic hydrolysis of small intestine mucosa is high and the liquid is cloudy in the process of extracting heparin sodium is solved.

Description

technical field [0001] The invention relates to a production process of heparin sodium, in particular to a production process of heparin sodium for pig small intestinal mucosa. Background technique [0002] Heparin is a mucopolysaccharide that widely exists in mammalian tissues, mainly exists in mast cells, has anticoagulant effect, and is widely used in the treatment of surgery, cerebral thrombosis, myocardial infarction, etc. The molecular weight of heparin is about 3000-37500, and research on commercial heparin suggests that there are at least 21 molecular entities. Heparin sodium is the sodium salt of heparin. As a natural anticoagulant substance, heparin sodium has been valued by countries all over the world. It is also one of the main export drugs in my country. With the deepening of research, it has been found that heparin sodium not only has anticoagulant, antithrombotic and blood lipid regulation effects, but also has anti Inflammation, anti-allergy, anti-virus, an...

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Problems solved by technology

[0003] At present, during the extraction process of heparin sodium, the enzymolysis solution is turbid after enzymolysis, and the protein content in the enzymolysis solution is high, which not only affects the quality and purity of heparin sodium, but also increases the processing of the enzymolysis solution such as adsorption and elution. Difficulty, in addition, in the prior art, the protein content in the waste liquid produced after extracting heparin sodium is high, which increases the environmental protection cost of sewage treatment

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