Production process of heparin sodium in porcine small intestine mucosa

A production process and heparin sodium technology, applied in the field of heparin sodium production technology, can solve problems such as increasing the processing difficulty of enzymatic hydrolysis solution adsorption and elution, increasing the environmental protection cost of sewage treatment, affecting the quality and purity of heparin sodium, etc., so as to save environmental protection. The effect of process, short adsorption time and thorough adsorption

Inactive Publication Date: 2019-03-26
胡晓辉
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, during the extraction process of heparin sodium, the enzymolysis solution is turbid after enzymolysis, and the protein content in the enzymolysis solution is high, which not only affects the quality and purity of heparin sodium, but also increases the processing of the enzymolysis solution such as adsorption and elution. Difficulty, in addition, in the prior art, the protein content in the waste liquid produced after extracting heparin sodium is high, which increases the environmental protection cost of sewage treatment

Method used

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  • Production process of heparin sodium in porcine small intestine mucosa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The heparin sodium production process of pig small intestine mucosa of the present invention comprises the following steps:

[0032] S1. Enzymolysis: Add the intestinal mucosa into the enzymolysis tank, add salt and stir for 10 minutes, then heat to 40-45°C, stop heating and stir for 10 minutes, adjust the pH value to 7.2-7.5 with sodium hydroxide, and stir for another 10 minutes Finally, add 2709 protease, stir for another ten minutes, then heat to 56-58°C, continue to stir for 30 minutes, then stop stirring and keep warm for 2 hours. -92°C, then keep warm for 30-60 minutes until the liquid in the enzymolysis tank is separated, the upper layer is protein, and the lower layer is clarified enzymolysis solution, separate the protein and enzymolysis solution, collect the enzymolysis solution and filter;

[0033] S2. Adsorption: After filtering the protein solution in the enzymolysis solution collected in step S1 by manger filtration, use clean water to reduce the temperatu...

Embodiment 2

[0042] Based on Example 1, the enzymatic hydrolysis in step S1 includes the following steps: adding the intestinal mucosa into an enzymatic hydrolysis tank, adding 3.5% of the intestinal mucosa weight and stirring for 10 minutes, then directly steaming and heating to 45°C, stopping the heating and stirring for 10 minutes Minutes, adjust the pH value to 7.2-7.5 with sodium hydroxide, stir for another 10 minutes, add 2709 protease, stir for another ten minutes, steam directly to 58 ° C, continue stirring for 30 minutes, stop stirring and keep warm for 2 hours, keep warm for 2 hours Finally, use steam direct heating and steam coil heating to quickly heat up to 85°C, turn off steam direct heating after the temperature rises to 85°C, use steam coil heating to slowly heat to 92°C, and then keep warm for 60 minutes to wait for the enzyme The liquid in the hydrolysis tank is layered, the upper layer is protein, and the lower layer is clarified enzymolysis solution, the protein and enzy...

Embodiment 3

[0044] The enzymolysis solution produced according to the enzymolysis method described in Example 2 is subjected to membrane filtration to obtain a treatment solution, and the components of the treatment solution and the unfiltered stock solution are detected. The unfiltered stock solution refers to the method described in Example 2. The enzymatic hydrolyzate produced by the enzymatic hydrolysis method, the test results are as follows:

[0045]

[0046] Conclusion: each index in the enzymolysis solution produced by the enzymolysis method of Example 2 of the present invention is better than that of the enzymolysis solution produced by the traditional method. In summary, it can be clearly seen that the enzymolysis solution produced by the present invention is clean and environmentally friendly, and contains The amount of protein and other impurities is small, and all the indicators of the treatment liquid after membrane filtration can reach the environmental protection value, ...

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Abstract

The invention discloses a production process of heparin sodium in porcine small intestine mucosa. The production process comprises the following steps that the intestinal mucosa is added into an enzymatic hydrolysis tank, stirring is performed for 10 minutes by adding salt and then heating is performed to reach the temperature of 40-45 DEG C, heating is stopped and stirring is performed for 10 minutes, the pH value is adjusted to 7.2-7.5 with a sodium hydroxide solution, 2709 protease is added after stirring is performed for another 10 minutes, stirring is performed for another 10 minutes andthen heating is performed to 56-58 DEG C, stirring is continued to be performed for 30 minutes and stirring is stopped and heat preservation is performed for 2 hours, temperature is raised to 85 DEG Cafter quick stirring after the heat preservation is performed for 2 hours, then heating is slowly performed to 90-92 DEG C after the temperature is raised to 85 DEG C, heat preservation is performedfor 30-60 minutes, the liquid in the enzymatic hydrolysis tank is layered, the upper layer is protein, the lower layer is a clear enzymatic hydrolysate, the protein and the enzymatic hydrolysate are separated, and the enzymatic hydrolysate is subjected to adsorption, washing, elution, precipitation and drying to obtain the crude heparin sodium. The problems that the content of protein contained inthe enzymatic hydrolysate after the enzymatic hydrolysis of small intestine mucosa is high and the liquid is cloudy in the process of extracting heparin sodium is solved.

Description

technical field [0001] The invention relates to a production process of heparin sodium, in particular to a production process of heparin sodium for pig small intestinal mucosa. Background technique [0002] Heparin is a mucopolysaccharide that widely exists in mammalian tissues, mainly exists in mast cells, has anticoagulant effect, and is widely used in the treatment of surgery, cerebral thrombosis, myocardial infarction, etc. The molecular weight of heparin is about 3000-37500, and research on commercial heparin suggests that there are at least 21 molecular entities. Heparin sodium is the sodium salt of heparin. As a natural anticoagulant substance, heparin sodium has been valued by countries all over the world. It is also one of the main export drugs in my country. With the deepening of research, it has been found that heparin sodium not only has anticoagulant, antithrombotic and blood lipid regulation effects, but also has anti Inflammation, anti-allergy, anti-virus, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
CPCC08B37/0075
Inventor 胡晓辉
Owner 胡晓辉
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