Method for detecting aflatoxin in corn feed stored in farm

Abstract

The invention discloses a method for detecting the aflatoxin in corn feed stored in a farm, including the following steps of: processing the corn feed before using it to obtain a powder sample; carrying out continuous reflux extraction on the powder sample to obtain a corn feed extract; and passing the corn feed extract through a micro-column chromatography tube composed of alumina and Si-Mg adsorbents, and irradiating with ultraviolet light having a wavelength of 365 nm to observe whether blue-violet fluorescence appears on the Si-Mg adsorption layer or not so as to judge whether this batch of corn feed can be used or not. The method for detecting the aflatoxin in corn feed stored in a farm is based on a micro-column screening method which induces and stimulates the stored corn feed sample, so that the aspergillus flavus possibly contained in the corn feed sample can heavily multiply and generate a large amount of aflatoxin. In this way, the aflatoxin can be easily detected by the micro-column screening method, so that the problem that the aflatoxin cannot be detected by the micro-column screening method due to low mildew and low aflatoxin content is avoided, and the detection precision is improved.

Description

technical field [0001] The invention relates to the field of livestock product feed detection, in particular to a method for detecting aflatoxin in corn feed stored in a farm. Background technique [0002] Corn is one of the main raw materials for animal feed, and a large amount of corn is often stored as feed in animal farms or animal feed production plants. Under normal circumstances, when these corn feeds are purchased, samples are generally sent to a special laboratory for testing to determine whether the aflatoxin in them exceeds the standard. However, during the storage process, some corn feed may become moldy due to reasons such as storage time, storage conditions and changes in the external environment, thereby producing aflatoxins. Since the storage period of corn feed is generally not too long, the number of moldy corn is very small, and the degree of mold is generally low. It is difficult to distinguish the presence of mold in corn feed from the surface. [0003...

AI Technical Summary

Problems solved by technology

[0020] The detection cost of this method is low, but it also has the problems of cumbersome operation, time-consuming, unsatisfactory extraction and purification effects, and poor sensitivity. Aflatoxin, which will lead to the accumulation of trace amounts of aflatoxin in animals, thereby endangering the human body

[0021] Since a large amount of corn feed stored in a farm or animal feed factory (especially an animal feed farm) is used in large quantities every day, if it is tested every time before use, not only the detection cost is high (need to purchase professional equipment and Training professional inspectors), and more importantly, due to the need for frequent use, the frequency of detection is very high, the existing detection methods are very cumbersome, and it is difficult to detect trace amounts of mold, resulting in trace amounts of aflatoxin in animals enrichment, thereby affecting food and personal safety

Images