Enriching method of microorganism microbial populations and application of enriching method to treatment of Cr heavy metal pollution

A technology of microbial flora and heavy metals, applied in the field of enrichment and cultivation of microbial flora, can solve the problems of strong toxicity

Inactive Publication Date: 2019-04-02
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this reduction process, Cr intermediates with a valence of pentavalent and acti

Method used

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  • Enriching method of microorganism microbial populations and application of enriching method to treatment of Cr heavy metal pollution
  • Enriching method of microorganism microbial populations and application of enriching method to treatment of Cr heavy metal pollution
  • Enriching method of microorganism microbial populations and application of enriching method to treatment of Cr heavy metal pollution

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for enriching microbial flora, which is characterized in that it comprises the following steps:

[0033] (1) Collect the mud bottom of the pond as the environmental bacteria source, put the bottom mud of the pond into a culture bottle, use potassium dichromate to adjust the Cr(VI) concentration in the culture bottle, and dilute the volume to 600 mL, and place it at 28°C after packaging. Cultivation is static for 30 days at temperature to obtain the first generation of enriched flora.

[0034] The physical and chemical properties of the pond bottom mud are 30.15g / kg organic matter, 1.33g / kg total nitrogen, 35.4mg / kg available phosphorus, and 71% water content.

[0035] (2) Transfer the obtained first-generation enriched flora to fresh PCS culture medium containing Cr(VI), and cultivate at 28°C for 7 days to obtain the second-generation enriched flora. This method was transferred 5 times to obtain the 5th generation enriched flora. Cr(VI) transformation ability is sta...

Embodiment 2

[0043] The bacterial colony was cultured in PCS medium containing Cr(VI), the initial pH of the culture medium was adjusted, and the ability of the bacterial colony to reduce Cr(VI) under different pH conditions was examined. The result is figure 2 As shown, when pH=7 and pH=8, the flora can effectively reduce Cr(VI) in the culture solution. When pH=7, after 73 hours of culture, the Cr(VI) concentration in the culture medium dropped from 92.15μg / mL in the initial culture to 2.19μg / mL, and 97.6% of Cr(VI) was reduced and transformed. When pH=8, after 73 hours of cultivation, the Cr(VI) concentration in the culture solution was 0μg / mL, and the reduction rate reached 100%.

Embodiment 3

[0045] Cultivate the flora in PCS culture medium of about 100μg / mL Cr(VI), adjust the pH=8, and detect the change of OD600 of the flora during the culture process, the results are as follows image 3 . OD600 can reflect the changes in biomass during the growth of microorganisms. by image 3 It can be found. Bacteria can start to grow in Cr(VI) solution, cultured for 24h, the OD reached 0.41, and with the increase of culture time, the biomass of the flora also increased, and reached 0.83OD at 92.5h.

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Abstract

The invention provides an enriching method of microorganism microbial populations. The enriching method comprises the following steps of using pond bottom mud microorganisms as an environment microbial source, performing continuous directional domestication and subculture screening on high-concentration Cr(VI), so as to finally obtain a group of microorganism microbial populations which can effectively reduce Cr(VI). The microbial populations can effectively reduce the Cr(VI), and within 49.5h, 91 [mu]g/mL of the Cr(VI) can be totally reduced. The microbial population can tolerate the Cr(VI) concentration environment of 435[mu]g/mL, and can maintain reducing capacity. Under the condition of 15-45 DEG C, the microbial populations have the capacity for reducing the Cr(VI), and under the condition of 30 DEG C and 35 DEG C, the efficiency of reducing the Cr(VI) is the best.

Description

Technical field [0001] The invention relates to a method for enriching and cultivating microbial flora, and applying the microbial flora to environments polluted by heavy metals containing Cr, including water environment, soil environment, and organic pollution environment, and belongs to the field of biotechnology. Background technique [0002] my country's heavy metal pollution is relatively serious. According to statistics, the area of ​​soil heavy metal pollution in my country is about 2×10 7 hm 2 , How big is the annual contaminated grain is 1.2×10 7 t, the economic loss reaches 2×10 10 Yuan (Zhao Qiguo, et al., Acta Pedologica Sinica, 2007, 44(6): 1127-1134). [0003] Chromium is one of the main heavy metal pollutants. Because it is an important raw material for some industries such as electroplating, wood preservation, dyes, leather production, and alloy manufacturing, it is widely used in industrial production, and with the discharge of industrial wastewater, a large amount...

Claims

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Application Information

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IPC IPC(8): C12N1/02C12N1/20C02F3/34B09C1/10C02F101/22
CPCB09C1/10C02F3/34C02F2101/22C12N1/02C12N1/20
Inventor 吕育财杨涛祁正李平王奔腾向振史子瑶龚大春张耀平李宁郭金玲田毅红
Owner CHINA THREE GORGES UNIV
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