A kind of cultivation method of cassava axillary bud somatic cell embryo in vitro culture virus-free seedling

Abstract

The invention provides a culture method for cassava axillary bud somatic embryogenesis in-vitro culture of virus-free seedlings. The method comprises the following steps of performing pre-culturing, wherein cassava tender stems infected with ACMV are subjected to conventional surface disinfection, then, stems with auxiliary buds or incompletely-detoxified stems with auxiliary buds infected with virus test tube seedlings are cut and inoculated to an auxiliary bud expansion induction culture medium for performing dark culture for 3-7 d; performing somatic embryogenesis induction culture, whereinauxiliary buds expanded after culture are stripped and inoculated on a somatic embryogenesis induction culture medium for performing dark culture for 25-35 d; performing somatic embryogenesis matureculture, wherein somatic embryogenesis tissue blocks are inoculated to a somatic embryogenesis mature culture medium for performing illumination culture for 25-35 d; performing plant regeneration culture, wherein mature somatic embryogenesis tissue blocks are inoculated to a plant regeneration culture medium for performing illumination culture for 28-40 d. The virus-free efficiency and the breeding coefficient are high, explant materials are multiple, operation is easy, speed is high, the number of regeneration plants is large, the seedling rooting rate is high, and seedlings grow robustly.

Description

Technical field [0001] The present invention relates to the culture method of a cellular embryogenic cultured detoxification seedlings of cassava marsh bracket cells. Background technique [0002] MANIHOT ESCULENTA CRANTZ is Euphorbiae plant, the chromosome is 2N = 36, and the born shrub is 1-5m. Cassava is second only to rice, corn, and sorghum, the roots contain rich starch, which can be used as food, can also be used as industrial raw materials or feed. It is a main food source of 600 million people in the tropical area. Cassava originated in South America, in the 16th-18th century, in Africa, Asia and Oceania. Cassava is now widely distributed around the world's tropical and partial subtropical regions. Cow potatoes were introduced to my country 1820, and there were more than 180 years of cultivation history. At present, my country's cassava producing area includes Hainan, Guangdong, Guangxi, Fujian, Yunnan and other places in Sichuan, Guizhou, Hunan, Jiangxi, etc., existing ...

AI Technical Summary

Problems solved by technology

In addition, the growth point of the stem tip is not only small in length, but also very tender. During the peeling process, it is difficult to quickly find the exact location, and it is easily broken and damaged during the peeling and transfer process. The operation is very difficult, which not only wastes materials , and difficult to mass-produce

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