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A kind of mole cricket extract and its extraction method

An extraction method and extraction technology, applied in the field of mole cricket extract and its extraction, can solve the problems of insignificant inhibition, complicated extraction process, and high cost, and achieve the effect of obvious inhibition, simple extraction process, and low cost

Active Publication Date: 2022-07-12
SHANGHAI INST OF PHARMA IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the defects in the prior art that the extraction process of the mole cricket drug active ingredient is complicated, the cost is high, and the inhibitory effect of the obtained mole cricket extract on the brain glioma cell U87 is not obvious, and a mole cricket is provided. Extract and its extraction method

Method used

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  • A kind of mole cricket extract and its extraction method
  • A kind of mole cricket extract and its extraction method

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Take 100 g of the pulverized dry medicinal powder of mole crickets, extract three times with 800 mL of petroleum ether (boiling range is 60-90 ° C) under reflux, and concentrate under vacuum at 60 ° C until there is no solvent smell, and the step yield is about 25%. It was then dissolved in 6 times the volume of 70% acetone aqueous solution, and then placed in the refrigerator for 12 hours. The lower layer solution was taken out and concentrated under reduced pressure at 60°C. The yield in this step was 25%; the total yield calculated by medicinal materials was 6.25%. . The obtained extract was subjected to in vitro inhibition test against glioma cells U87.

Embodiment 2

[0043] Take 100 g of the pulverized dry medicinal powder of mole crickets, extract three times with 600 mL of cyclohexane under reflux, and concentrate under vacuum at 60° C. until there is no solvent smell. The yield in this step is about 25%. It was then dissolved in 4 times the amount of 80% acetone aqueous solution, and then placed in the refrigerator for 10 hours. The lower layer solution was taken out and concentrated under reduced pressure at 60 ° C under vacuum. The yield of this step was about 20%, and the obtained extract was subjected to anti-brain In vitro inhibition assay of glioma cells U87.

Embodiment 3

[0045] Take 100 g of the pulverized dry crude medicinal powder of mole crickets, extract 4 times with 1000 mL of hexane under reflux, and concentrate under vacuum at 60° C. until there is no solvent smell. The yield in this step is about 25%. It was then dissolved in 8 times the amount of 60% acetone aqueous solution, and then placed in the refrigerator for 12 hours. The lower layer solution was taken out and concentrated under reduced pressure at 60 ° C under reduced pressure. The yield of this step was about 22%. The obtained extract was subjected to anti-brain In vitro inhibition assay of glioma cells U87.

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Abstract

The invention discloses a method for extracting a mole cricket extract. The extraction method includes the following steps: extracting the mole crickets with a low-polarity solvent, then concentrating under reduced pressure, mixing with the acetone aqueous solution or the methyl ethyl ketone aqueous solution until the extract has no solvent smell, then refrigerating, removing the lower layer liquid, and concentrating and drying. Mole cricket extract. The extraction process of the invention is simple, the cost is low, and the pollution is less, and the extracted mole cricket extract has obvious inhibitory effect on the brain glioma cell U87.

Description

technical field [0001] The invention relates to a mole cricket extract and an extraction method thereof. Background technique [0002] The mole cricket is an insect of the family Orthoptera. Among them, the North China mole cricket in China has medicinal value, and it is recorded in some ancient medical books that it can be used for diuresis and detoxification, swelling and detoxification, stone stranguria, scrofula, and malignant sores. [0003] Glioma is the most common primary brain tumor arising from the canceration of glial cells in the brain and spinal cord. The annual incidence is about 3-8 per 100,000 population. Clinical experience has found that gliomas are difficult to cure and often recur. After tumor recurrence, reoperation, radiotherapy, chemotherapy and other treatments are required according to the functional status of the patient. CN1582970A mentioned that the effective parts extracted from mole crickets can be used to prepare antitumor drugs. [0004] ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/64A61P35/00
CPCA61K35/64
Inventor 杨培明李睿
Owner SHANGHAI INST OF PHARMA IND CO LTD