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Method for repairing arsenic-polluted soil by inducing active iron and manganese oxides through bacteria under aerobiotic/microaerobic conditions

An iron-manganese oxide, inducing activity technology, applied in the restoration of polluted soil and other directions, can solve the problems of easy migration of arsenic and limited arsenic repair ability, and achieve the effects of low treatment cost, convenient operation and simple process

Active Publication Date: 2019-04-05
QINGDAO TECHNOLOGICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problem of the above-mentioned microbial remediation technology is that the ability to remediate arsenic is very limited, and arsenic is easily migrated.

Method used

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  • Method for repairing arsenic-polluted soil by inducing active iron and manganese oxides through bacteria under aerobiotic/microaerobic conditions
  • Method for repairing arsenic-polluted soil by inducing active iron and manganese oxides through bacteria under aerobiotic/microaerobic conditions
  • Method for repairing arsenic-polluted soil by inducing active iron and manganese oxides through bacteria under aerobiotic/microaerobic conditions

Examples

Experimental program
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Effect test

Embodiment example 1

[0027] 1) Enrichment culture of bacteria

[0028] Pseudomonas putida P. putida Strain MnB1 was inoculated in Pseudomonas putida enrichment medium A at a volume ratio of 5%, and then aerobic enrichment culture was carried out at 25 degrees Celsius (150 rpm) for 2 days;

[0029] The components of the medium A are 0.5 g of yeast extract powder, 0.5 g of hydrolyzed casein, 0.5 g of glucose, 0.29 g of calcium chloride, 0.5 g of magnesium sulfate, 1 mL of trace elements, and 1 liter of deionized water;

[0030] The selected bacterial strains come from ATCC, the American Type Bacteria Collection Center, with the preservation number ATCC 23483;

[0031] 2) Preparation of active metabolites:

[0032] Add the Pseudomonas putida bacteria solution enriched in step 1) to the Pseudomonas putida culture medium B with an initial concentration of manganese carbonate of 1 g / L and a pH of 6.8 at an inoculum of 6% by volume, and then in 25 Celsius, cultivated under aerobic conditions for 4 day...

Embodiment example 2

[0038] 1) Enrichment culture of bacterial strains:

[0039] Pseudomonas putida P. putida Strain MnB1 was inoculated in Pseudomonas putida enrichment medium A at a volume ratio of 10%, and then aerobic enrichment culture was carried out at 25 degrees Celsius for 5 days under horizontal shaking (150 rpm);

[0040] The components of medium A are 0.5g of yeast extract powder, 0.5g of hydrolyzed casein, 0.5g of glucose, 0.2g of calcium chloride, 0.3g of magnesium sulfate, 4mL of trace elements, and 1 liter of deionized water;

[0041] The selected bacterial strains come from the American Type Bacteria Collection Center, with the preservation number ATCC 23483;

[0042] 2) Preparation of active iron manganese oxide:

[0043] Add the Pseudomonas putida bacteria solution enriched in step 1) to the Pseudomonas putida culture medium B containing manganese carbonate with an initial concentration of 1.0g / L and a pH of 6.8 at an inoculation volume of 10% by volume, and then in 30 degree...

Embodiment example 3

[0048] 1) Enrichment culture of bacterial strains:

[0049] Pseudomonas putida P. putida Strain MnB1 was inoculated in Pseudomonas putida enrichment medium A at a volume ratio of 8%, and then aerobic enrichment culture was carried out at 20 degrees Celsius (120 rpm) for 5 days;

[0050] The composition of medium B is 0.35g of yeast extract powder, 0.35g of hydrolyzed casein, 0.35g of glucose, 0.2g of calcium chloride, 0.5g of magnesium sulfate, 2mL of trace elements, and 1 liter of deionized water;

[0051] The selected bacterial strains come from the American Type Bacteria Collection Center, with the preservation number ATCC 23483;

[0052] 2) Preparation of active iron manganese oxide:

[0053] Add Pseudomonas putida culture medium B containing manganese carbonate with an initial concentration of 1.0g / L and a pH of 7.0 to the inoculum of Pseudomonas putida obtained in step 1) at a volume ratio of 7%. The ingredients are 0.25g of ferrous ammonium sulfate, 0.25g of sodium c...

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Abstract

The invention relates to a method for repairing arsenic-polluted soil by inducing active iron and manganese oxides through bacteria under aerobiotic / microaerobic conditions. The method comprises the steps of selecting a Pseudomonas putida strain (MnB1, ATCC 23483), culturing the strain in a culture medium containing manganese carbonate for 3-10 days until a brownish black flocculent product is generated by bacterial induction, then adding a metabolite in arsenic-polluted soil, and culturing for 3-21 days under the aerobiotic / microaerobic conditions. Under the effect of bacterial metabolites, arsenic which is in an exchangeable form in the soil can be transformed into arsenic which is in a residue form, migration and bioavailability of arsenic in the soil are reduced, and the arsenic in thesoil is fixed effectively. The process is simple to operate, low in treatment cost, wide in application range and free of secondary pollution.

Description

technical field [0001] The invention belongs to the technical field of soil remediation treatment for environmental protection and relates to a method for remediating arsenic-contaminated soil by bacteria-induced active iron-manganese oxides under aerobic / micro-aerobic conditions. technical background [0002] Arsenic (As) is a highly toxic metalloid that can cause skin cancer, bladder cancer, and kidney disease, posing a serious threat to human health. Due to the extensive use of sewage irrigation, industrial sludge and arsenic-containing pesticides in agricultural production, the soil is seriously polluted by arsenic. Studies have shown that arsenic-contaminated soil is mainly composed of inorganic trivalent arsenic and pentavalent arsenic, and in the soil environment, the mobility of trivalent arsenic is higher than that of pentavalent arsenic. [0003] There are three main categories of arsenic-contaminated soil remediation technologies: physical-chemical remediation, p...

Claims

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Application Information

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IPC IPC(8): B09C1/08B09C1/10
CPCB09C1/08B09C1/10
Inventor 王华伟王亚楠孙英杰高莹
Owner QINGDAO TECHNOLOGICAL UNIVERSITY
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