Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Kit for detecting enterovirus and distinguishing coxsackievirus A16 from enterovirus 71

A coxsackie virus and enterovirus technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganism measurement/testing, etc., can solve unfavorable rapid diagnosis, virus particle integrity, antiserum titer host Issues such as the influence of cell sensitivity and long time for result interpretation

Inactive Publication Date: 2019-04-09
SHANGHAI XINGYAO MED TECH DEV CO LTD
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the integrity of virus particles, the strength of antiserum titers, and the sensitivity of host cells all affect the detection results, and the whole experiment requires double serum, which takes a long time to interpret the results, which is not conducive to rapid diagnosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Enterovirus Universal Type, Enterovirus 71 Type and Coxsackievirus A16 Type Nucleic Acid Detection Kit (Fluorescent PCR Method)

[0062] 1. Primer Probe Synthesis

[0063] Entrust Yingwei Jieji (Shanghai) Trading Co., Ltd. to synthesize the primer probe. The sequence of the synthesized primer probe is as follows:

[0064] The upstream primers are:

[0065] 5'-CCCTGAATGCGGMTAATC-3' is shown in SEQ ID No.3;

[0066] Downstream primers are:

[0067] 5'-ATTGTCACCATAAGCAGCCA-3' is shown in SEQ ID No.4;

[0068] The probe sequence is:

[0069] 5'-CGGAACCGACTACTTTGGGTGTCC-3', the 5' end is marked with FAM, and the 3' end is marked with BHQ1, as shown in SEQIDNo.5

[0070] 5'-AGCACACGCCCACAAGCCAGC-3', the 5' end is marked with JOE fluorescent group, and the 3' end is marked with BHQ1, as shown in SEQIDNo.6

[0071] 5'-AGCGCGCACCCTCAAYCCAGG-3', the 5' end is labeled with ROX fluorescent group, and the 3' end is labeled with BHQ1, as shown in SEQIDNo.7

[0072] A...

Embodiment 2

[0090] Application of Example 2 Enterovirus Universal Type, Enterovirus 71 Type and Coxsackievirus A16 Type Nucleic Acid Detection Kit (Fluorescent PCR Method)

[0091] Adopt the gained kit of the present invention to test 20 routine clinical samples, carry out parallel comparative test with EV71, CA16 and universal enterovirus nucleic acid detection kit (fluorescence PCR method) produced by Jiangsu Mole Biotechnology Co., Ltd. simultaneously, evaluate reagent box effect.

[0092] 1. Enterovirus universal, enterovirus 71 and coxsackievirus A16 nucleic acid detection kits (fluorescence PCR method) for detection of clinical samples

[0093] (1) Sample extraction

[0094] Take clinically collected throat swabs, rectal swabs, herpes fluid, feces, and tissue fluid samples (the swabs are first rinsed with 1ml of normal saline), and 400 μl of the samples are respectively placed in wells A1-H1 and A7-H7 of the nucleic acid extraction plate. Before adding samples, add 5 μl of interna...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a kit for detecting enterovirus and distinguishing coxsackievirus A16 from enterovirus 71. According to the invention, a TaqMan probe multicolor fluorescent PCR technology is adopted, a universal primer for enterovirus is designed in a relatively conserved sequence of viral sequences, and a universal type of enterovirus, enterovirus 71 and coxsackievirus A16 are detected byspecific probes. According to the kit, the enterovirus can be detected only by virtue of the detection of different fluorescent labeled probes in the detection process without melting curve analysis after the fluorescence PCR, and the enterovirus 71 can be distinguished from the coxsackievirus A16. At the same time, the invention further introduces an artificially constructed internal reference system, so that false negative detection results can be avoided; and the kit is high in detection sensitivity, stable and reliable in results, easy to use, and suitable for environment, disease controland preservation as well as rapid detection of clinical enterovirus, coxsackievirus A16 and enterovirus 71.

Description

technical field [0001] The invention is a kit for detecting enterovirus and distinguishing coxsackievirus A16 and enterovirus 71. Background technique [0002] Hand, foot and mouth disease is a childhood infectious disease, also known as exanthematous vesicular stomatitis. It mostly occurs in children under 5 years old. The incubation period is usually 2-10 days, with an average of 3-5 days. It can cause herpes on the hands, feet, mouth and other parts. Most of the children recover by themselves in about a week, and a few children can cause myocarditis, etc. Complications can lead to death in severely ill children. The disease is the main clinical symptom with the formation of ulcers after herpes or ulceration of the hands, feet and oral mucosa. Common cases manifest as: acute onset, fever, mouth pain, anorexia, oral mucosa with scattered herpes or ulcers, maculopapules on hands, feet, buttocks, arms, and legs, and then turn into herpes. The number of rashes ranges from a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/686C12R1/93
CPCC12Q1/701C12Q1/686C12Q2600/166C12Q2561/101C12Q2521/531C12Q2545/101
Inventor 郭圣明吴大治夏懿
Owner SHANGHAI XINGYAO MED TECH DEV CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com