Kit for detecting enterovirus and distinguishing coxsackievirus A16 from enterovirus 71

Abstract

The invention provides a kit for detecting enterovirus and distinguishing coxsackievirus A16 from enterovirus 71. According to the invention, a TaqMan probe multicolor fluorescent PCR technology is adopted, a universal primer for enterovirus is designed in a relatively conserved sequence of viral sequences, and a universal type of enterovirus, enterovirus 71 and coxsackievirus A16 are detected byspecific probes. According to the kit, the enterovirus can be detected only by virtue of the detection of different fluorescent labeled probes in the detection process without melting curve analysis after the fluorescence PCR, and the enterovirus 71 can be distinguished from the coxsackievirus A16. At the same time, the invention further introduces an artificially constructed internal reference system, so that false negative detection results can be avoided; and the kit is high in detection sensitivity, stable and reliable in results, easy to use, and suitable for environment, disease controland preservation as well as rapid detection of clinical enterovirus, coxsackievirus A16 and enterovirus 71.

Description

technical field [0001] The invention is a kit for detecting enterovirus and distinguishing coxsackievirus A16 and enterovirus 71. Background technique [0002] Hand, foot and mouth disease is a childhood infectious disease, also known as exanthematous vesicular stomatitis. It mostly occurs in children under 5 years old. The incubation period is usually 2-10 days, with an average of 3-5 days. It can cause herpes on the hands, feet, mouth and other parts. Most of the children recover by themselves in about a week, and a few children can cause myocarditis, etc. Complications can lead to death in severely ill children. The disease is the main clinical symptom with the formation of ulcers after herpes or ulceration of the hands, feet and oral mucosa. Common cases manifest as: acute onset, fever, mouth pain, anorexia, oral mucosa with scattered herpes or ulcers, maculopapules on hands, feet, buttocks, arms, and legs, and then turn into herpes. The number of rashes ranges from a...

AI Technical Summary

Problems solved by technology

However, the integrity of virus particles, the strength of antiserum titers, and the sensitivity of host cells all affect the detection results, and the whole experiment requires double serum, which takes a long time to interpret the results, which is not conducive to rapid diagnosis