Composite microorganism deodorizing agent and preparation method and applications thereof

A technology of compound microorganisms and deodorant bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of non-reuse, secondary pollution of the environment, and increased costs, and achieve the promotion of removal of NH3 and The effect of the effect of H2S

Active Publication Date: 2019-04-12
ECOLOGY INST SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two technologies are easy to increase the cost while deodorizing qui

Method used

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  • Composite microorganism deodorizing agent and preparation method and applications thereof
  • Composite microorganism deodorizing agent and preparation method and applications thereof
  • Composite microorganism deodorizing agent and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: the isolation of deodorizing bacterial strain

[0058] Take 10g of manure pile outside a pig farm, put it into a triangular flask containing sterilized glass beads and 50ml of normal saline, shake it at 30°C, 160r / min for 2h, let it stand for 20min, take 1ml of supernatant, and use sterilized Physiological water diluted to a concentration of 10 -1 The suspension was serially diluted 10 times, and 200 μl of suspensions with different dilutions were drawn, spread on MRS agar medium, nutrient broth agar medium, and YPD agar medium respectively, and cultured at 28°C for 28-72 hours to observe the growth of the strains. Growth status, pick a single colony for culture. According to the shape and growth of the strains, the strains with different shapes were picked for streak culture and stored at low temperature. A total of 40 strains of bacteria were isolated in the experiment, including 26 strains of bacteria, 8 strains of yeast and 6 strains of lactobacilli....

Embodiment 2

[0063] Embodiment 2: Screening and identification of bacterial strains

[0064] 1 Primary screening of strains

[0065] Add 150g of fresh pig manure into a 500ml Erlenmeyer flask with a stopper, inoculate the isolated bacterial strain according to an inoculation amount of 5% by weight to volume, cultivate it for 10 days in a 30°C incubator, and use the sensory method to evaluate the odor level, the result See Table 1. It can be seen from Table 1 that the deodorizing ability of the isolated microorganisms is different. Through comparison, it is found that three strains of bacteria, one strain of lactic acid bacteria and two strains of yeast have better deodorizing ability.

[0066] The odor level is determined by the sensory method, and the gas odor level is divided by the 6-level method:

[0067] Level 0: no odor, represented by "-";

[0068] Grade 1: Barely feel the smell, indicated by "+";

[0069] Level 2: weak odor, represented by "++";

[0070] Level 3: Obvious odor,...

Embodiment 3

[0110] Embodiment 3: the compounding of microbial deodorant

[0111] (1) Antagonism experiment

[0112] The obtained three deodorizing bacterial strains were crossed between two pairs on the nutrient solid medium by the method of crossing and crossing, and the growth of the strains at the crossing point was observed. If the intersection point of the two grows, it means that there is no antagonism, otherwise, it means that there is antagonism between the strains. It was found that there was no antagonism among the three strains.

[0113] (2) Compounding of deodorizing strains

[0114] Inoculate the obtained three strains of deodorizing bacteria into nutrient broth, YPD, and MRS liquid medium, respectively, and cultivate them on a shaking table until the logarithmic growth phase, and carry out the culture solution of the three strains according to the following ratio (bacillus: yeast: lactic acid bacteria). Compounding: A: 0:1:1; B: 1:0:1; C: 1:1:0; D: 1:1:1; E: 1:2:2; F: 2:1...

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Abstract

The invention relates to a composite microorganism deodorizing agent and a preparation method and applications thereof. The microorganism agent includes bacillus cereus 2-2, pichia manshurica 2-6 andlactobacillus zeae 2-20, wherein the total bacteria count of the composite microorganism deodorizing agent is 2x10<9> cells/mL. By combining the three strains of the bacillus cereus 2-2, the pichia pastoris 2-6 and the lactobacillus cornicula 2-20, the method finds that the three strains not only have no antagonistic reaction, but also can significantly improve the effect of removing NH3 and H2S.Actual test indicates that the ammonia removal rate of the microorganism deodorizing agent compounded of the three strains reaches 73.04%, and the removal rate of hydrogen sulfide reaches 71.19%.

Description

technical field [0001] The invention relates to a compound microbial deodorizing agent and its preparation method and application, belonging to the technical field of harmless treatment of livestock and poultry manure or biological deodorization. Background technique [0002] With the continuous development of my country's livestock and poultry industry, the degree of intensification is getting higher and higher, the number of livestock and poultry breeding has increased sharply, and the discharge of manure is also increasing, causing certain pollution to air, water and soil. Among them, odor pollution is considered to be one of the six major public hazards next to noise pollution, which not only seriously pollutes the environment, but even endangers people's health, and has the duality of air pollution and harmful gas pollution. A large amount of livestock and poultry manure is fermented during the stacking process to produce various odorous substances, including NH 3 、H ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C02F11/02C12R1/085C12R1/84C12R1/225
CPCC12R2001/225C12N1/205
Inventor 陈贯虹黄玉杰张强王加宁高永超张闻王磊磊赵庆庆郑立稳
Owner ECOLOGY INST SHANDONG ACAD OF SCI
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