Distant grafting method of arabidopsis thaliana and nicotiana benthamiana
A technology of Nicotiana benthamiana and Arabidopsis, which is applied in the field of grafting of small plants, can solve the problems of the difficulty and risk of Arabidopsis and tobacco grafting system, and the high toxicity of nicotine, so as to achieve high survival rate of grafting, fast wound healing, Simple Effects of Genetic Background
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Embodiment 1
[0023] (1) Disinfection and germination of Arabidopsis and tobacco seeds: Take a 1.5mL centrifuge tube, mark the names of Arabidopsis and Nicotiana benthamiana seeds with a pencil on the cap and wall of the centrifuge tube, take an appropriate amount of seeds and put them into the corresponding centrifuge tube. The tube was treated with chlorine gas for 1 hour, and then the aseptic seeds were planted on a sterile petri dish containing solid medium on the ultra-clean workbench, and placed in a culture room for 7-9 days. The growth conditions of the culture room were: the temperature was controlled at 23±1℃, relative humidity 70%, long-day conditions, 16h light and 8h dark treatment.
[0024] (2) Select a robust and complete Arabidopsis plant as a scion; under the same conditions, select a robust and complete tobacco plant as a rootstock.
[0025] (3) Grafting under sterile conditions: Put the prepared Arabidopsis scion on the moist and sterile whatman paper on the ultra-clean w...
experiment example
[0066] 20 strains are all grafted in above embodiment 1 and comparative example 1-comparative example 5, respectively observe grafting survival strain number and count as follows:
[0067] Table 1
[0068]
[0069] It can be seen from the above table 1 that the growth conditions after the completion of the grafts have a great influence on the healing of the grafts. Comparative examples 1-3 were all completed on a sugar-free medium, but no fully healed grafts appeared after 4 days of grafting. Embodiment 4-6 is all completed on aseptic paper, because the common filter paper moisture retention property that adopts in the comparative example 4 is not good, there is no graft that heals complete; Comparative example 5 (adopts moist and aseptic A4 printing paper) Although there were grafts that healed completely, the later growth of the grafts had a greater impact. In Example 1, the survival rate of the fully healed graft was as high as 90%. It can be seen from the above table ...
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