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Method for preparing composite microbial flocculant by using composite flocculating bacteria

A technology of microbial flocculants and composite bacterial agents, which is applied in chemical instruments and methods, biological water/sewage treatment, special compound water treatment, etc., can solve the problems of limiting the application of microbial flocculants, unsatisfactory treatment effects, and high cultivation costs. Achieve the effects of genetically stable strains, reduced intake of organic nitrogen sources, and fast growth

Active Publication Date: 2020-08-07
HARBIN INST OF TECH AT WEIHAI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the research in this area is aimed at a single bacterial group, and the disadvantages of high culture cost and unsatisfactory treatment effect limit the application of microbial flocculants.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the preparation of composite type flocculation bacteria:

[0024] (1) Activation of strains: Prepare common beef extract peptone medium, sterilize at 121°C for 20 min, pick single colonies of flocculating bacteria S2-M-5 and S2-F-5 in beef extract peptone liquid with inoculation loops The culture medium was cultured in a shaker at 28°C and 120 r / min, and after 24 hours, the activation solutions of flocculation bacteria S2-M-5 and S2-F-5 were obtained respectively;

[0025] (2) Mix the activation solutions of flocculation bacteria S2-M-5 and S2-F-5 at a ratio of 2:1 to prepare composite flocculation bacteria.

[0026] The preparation method of the composite flocculation bacteria described in the embodiment of the present invention, the flocculation effect of the composite microbial flocculant obtained by fermentation of the composite bacteria is stable, and the flocculation rate can reach 83.62%, which is higher than the flocculation rate of the flocculant ...

Embodiment 2

[0027] Embodiment 2: the preparation of composite microbial flocculant, utilize the composite flocculation bacterium described in embodiment 1, concrete operation is as follows:

[0028] (1) Preparation of flocculation fermentation medium: Weigh 12.82g of sucrose, 4.00g of dipotassium hydrogen phosphate, 3.70g of magnesium sulfate, 3.00g of potassium dihydrogenphosphate, 33.00g of sodium chloride, and 0.52g of ammonium sulfate in 1L deionized In water, the pH value was adjusted to 6.5, placed in a sterilizing pot at 121°C, and sterilized for 20 min;

[0029] (2) Set the total inoculum amount to 4%, inoculate the composite flocculation bacteria into the flocculation fermentation medium in step (1), and culture in a shaker at 28°C and 120 r / min for 3 days;

[0030] (3) Put the fermentation broth in a centrifuge, centrifuge at 8000 r / min for 15 minutes, and the supernatant is the composite microbial flocculant;

[0031] (4) Add 200 mL of 4 g / L kaolin suspension, 2 mL of compound...

Embodiment 3

[0033] Embodiment 3: Utilize the compound type flocculation bacteria described in embodiment 1, concrete operations are as follows:

[0034] (1) Preparation of flocculation fermentation medium: Weigh 10.5g of sucrose, 4.00g of dipotassium hydrogen phosphate, 3.00g of magnesium sulfate, 3.00g of potassium dihydrogenphosphate, 33.00g of sodium chloride, and 0.52g of ammonium sulfate, and remove them in 1L. Adjust the pH value to 7.5 in ionized water, place in a sterilizing pot at 121°C, and sterilize for 20 min;

[0035] (2) The total inoculum amount was set at 4%, and the composite flocculation bacteria were inoculated into the prepared flocculation fermentation medium, and cultured in a shaker at 28°C and 120 r / min for 4 days;

[0036] (3) Place the fermentation broth in a centrifuge and centrifuge at 8000 r / min for 20 min. The supernatant is the composite microbial flocculant;

[0037] (4) Add 200 mL of 4 g / L kaolin suspension, 2 mL of compound microbial flocculant and 300 ...

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Abstract

The invention discloses a method for preparing a composite microbial flocculating agent by using composite flocculating bacteria, and belongs to the technical field of sewage treatment. The application of the microbial flocculating agent is limited by the disadvantages of high cultivation cost and non-ideal treatment effect of a single flora. Strains are activated separately, then compounded, andinoculated to a fermentation medium, fermental cultivation is carried out, a liquid supernatant is removed after centrifugalization, and the composite microbial flocculating agent is obtained; the composite microbial agent comprises pseudoalteromonas (Pseudoalteromonas hodoensis) S2-M-5 and pseudoalteromonas (Pseudoalteromonas nigrifaciens) S2-F-5 of which the preservation number is CGMCC No. 14429. The method for preparing the composite microbial flocculating agent by using the composite flocculating bacteria has the advantages that the flocculation effect is better, the flocculation rate canreach 93% or above, and the action condition is stable.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment, and in particular relates to a preparation method of a composite microbial flocculant. Background technique [0002] Due to the vigorous development of the mariculture industry, the pollution problems caused by it have also received people's attention. One of the main pollution problems is that the wastewater contains too much suspended colloidal substances. These suspended substances are mainly derived from excess bait, excrement and animal metabolites. It is necessary to add flocculants to destabilize the suspended substances and then flocculate them into large particles and settle down. This is an important link in the treatment of mariculture wastewater. [0003] Flocculants mainly include inorganic flocculants, organic flocculants and microbial flocculants. The most commonly used inorganic flocculants (aluminum and iron salts) will bring a series of problems, such as aluminum salt-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/00
CPCC02F3/00C02F2305/06
Inventor 王凯李斯琪
Owner HARBIN INST OF TECH AT WEIHAI
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