Fungus with function of degrading insoluble inorganic phosphorus and application thereof
An insoluble, inorganic phosphorus technology, applied in fungi, solid waste removal, microorganism-based methods, etc., to achieve large-scale application, reduce recycling costs, and avoid a lot of waste.
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[0024] This embodiment provides a kind of Paecilomyces lilacinus, its morphological characteristics: the strain is cultured on PDA medium at 30°C for 7 days, the diameter of the colony is 4.5-5.5 cm, the colony is a regular circle, and the color of the outer circle of the colony is white , the raised part in the middle is lavender, and the longer the time, the darker the color. The colonies are not easy to stir up and are opaque. The conidiophores are bottle-shaped under the scanning electron microscope. The spores are solitary or aggregated into spore bundles, with wheel-shaped branches. The base of the bottle peduncle is nearly cylindrical and the top is slender. Conidia are colorless, smooth, of different sizes, elliptic, 2-8×2-4μm, strung together, forming long spore chains that diverge at the base. The strain was deposited in the China Center for Type Culture Collection (Address: Wuhan University, Wuhan, China) on April 27, 2018, with the preservation number: CCTCC NO: M2...
experiment example 1
[0039] Experimental example 1. Determination of phosphorus solubilizing ability under solid culture conditions
[0040] Spot the isolated and purified strains on the inorganic phosphorus-selective solid medium plate, culture at 30°C for 7 days, observe the formation of the phosphorus-dissolving circle, and measure the diameter of the phosphorus-dissolving circle (D) and the diameter of the colony (d) by the cross method , and calculate the ratio of dissolved phosphorus circle diameter to colony diameter (D / d).
[0041] The test proved that the (D / d) value of the GZP5 strain was 1.15±0.003.
experiment example 2
[0042] Experimental Example 2. Determination of Phosphorus Solubilizing Ability under Liquid Culture Conditions
[0043] Add 1 mL of GZP5 strain seed solution into a sterilized Erlenmeyer flask containing 100 mL of inorganic phosphorus selective liquid medium. After inoculation, they were cultured in a shaker at 30°C and 160r / min for 7 days. At the same time, 1 mL of sterile water was added as a blank control, and three groups were set up in parallel. Samples were taken every 24 hours, and the OD700 value was measured by the molybdenum-antimony anti-colorimetric method, and the available phosphorus content in the supernatant was calculated according to the phosphorus content standard curve.
[0044] The result is as Figure 5 , the test proved that the available phosphorus content in the strain GZP5 culture solution rose to the highest at 72h, which was 98.09μg / mL.
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