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Method for synthesizing phenyllactic acid through microbial fermentation based on colloidal crystals

A microbial fermentation and phenyllactic acid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low conversion efficiency of strains, low yield of phenyllactic acid, and difficult separation, and achieve mass transfer and diffusion Rapid, simple fermentation process and broad application prospects

Inactive Publication Date: 2019-04-16
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the microbial fermentation process is limited by substrate inhibition, small cell concentration, low strain transformation efficiency, expensive substrates, low yield of phenyllactic acid, low concentration, and difficult separation, so it is urgent to study and solve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The Lactobacillus paracasei strain was activated in 30 mL MRS liquid medium for 24 hours to obtain seed liquid; 30 mL of seed liquid was inoculated into 500 mL MRS liquid medium, and the culture liquid was obtained by standing culture for 8 hours; 1.7 g of crystal gel Put it into the culture solution and leave it to stand for 16 hours, remove the crystal gel and drain the surface water of the crystal gel to obtain a seed crystal gel carrier. Take 1.5 mL of seed crystal gel carrier and put it into 30 mL MRS liquid medium to continue fermentation. The dry cell weight of Lactobacillus paracasei in crystal gel carrier reaches 3.43 mg / mL. After 12 hours of fermentation, the fermentation broth is subjected to high performance liquid chromatography Detection and analysis showed that the concentration of phenyllactic acid reached 73 mg / L.

Embodiment 2

[0028] The Lactobacillus paracasei strain was activated in 30 mL MRS liquid medium for 24 hours to obtain seed liquid; 30 mL of seed liquid was inoculated into 500 mL MRS liquid medium, and the culture liquid was obtained by standing culture for 8 hours; 1.7 g of crystal gel Put it into the culture solution and leave it to stand for 16 hours, remove the crystal gel and drain the surface water of the crystal gel to obtain a seed crystal gel carrier. Take 1.5 mL seed crystal gel carrier and put it into 30 mL MRS liquid medium to continue fermentation. The dry cell weight of Lactobacillus paracasei in crystal gel carrier reaches 6.43 mg / mL. After 36 hours of fermentation, the fermentation broth is subjected to high performance liquid chromatography Detection and analysis showed that the concentration of phenyllactic acid reached 90 mg / L.

Embodiment 3

[0030] The Lactobacillus paracasei strain was activated in 30 mL MRS liquid medium for 24 hours to obtain seed liquid; 30 mL of seed liquid was inoculated into 500 mL MRS liquid medium, and the culture liquid was obtained by standing culture for 8 hours; 1.7 g of crystal gel Put it into the culture solution and leave it to stand for 16 hours, remove the crystal gel and drain the surface water of the crystal gel to obtain a seed crystal gel carrier. Take 1.5 mL of seed crystal gel carrier and put it into 30 mL MRS liquid medium to continue fermentation. The dry cell weight of Lactobacillus paracasei in crystal gel carrier reaches 6.67 mg / mL. After 60 hours of fermentation, the fermentation broth is subjected to high performance liquid chromatography Detection and analysis showed that the concentration of phenyllactic acid reached 95 mg / L.

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Abstract

The invention discloses a method for synthesizing phenyllactic acid through microbial fermentation based on colloidal crystals. According to the method, the icon exchange colloidal crystals are adopted as a carrier, the adsorption function of the colloidal crystals on lactobacillus casei cells is utilized, a high-concentration whole-cell catalyst is formed in the fermentation process, substrate inhibition is relieved through the high-concentration whole-cell catalyst, the biomass of the fermentation process is increased, and then the yield of phenyllactic acid is increased. Phenyllactic acid obtained through the method is higher than that obtained through conventional free cell fermentation, phenyllactic acid in fermentation liquid is easy separate, the fermentation process is simple, operation is convenient, high safety is achieved, amplification is easy, and the method has wide application prospects in the field of biochemical synthesis.

Description

Technical field [0001] The invention belongs to the field of biochemical industry, and specifically relates to a method for synthesizing phenyllactic acid by microbial fermentation based on crystal gel. Background technique [0002] Phenyllactic acid is a new broad-spectrum antibacterial agent and an important monomer for the synthesis of new polyphenyllactic acid materials. It can also be used as an intermediate for synthetic drugs. It has broad applications in many fields such as chemical industry, new materials, food industry and pharmaceuticals. Application prospects. [0003] Phenyllactic acid can be synthesized by chemical methods such as phenylpyruvic acid catalytic hydrogenation, asymmetric epoxidation synthesis, zinc amalgam reduction, etc. However, it often requires the use of precious metal catalysts, highly solvents, hazardous gases, high temperature and high pressure and other harsh reaction conditions. There are many reaction steps, the process consumes energy, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/42C12R1/225
CPCC12P7/42
Inventor 吕妍婷许娜潘曼曼贠军贤
Owner ZHEJIANG UNIV OF TECH
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