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Nano gene carrier for in-vivo targeting tumor imaging and treatment and preparation method and application thereof

A tumor imaging and gene carrier technology, which can be used in preparations for in vivo experiments, gene therapy, anti-tumor drugs, etc. It can solve the problems of poor hydrophilicity and photostability of fluorescent molecules, and achieve good hydrophilicity , Improve the targeting ability, the effect of good biocompatibility

Active Publication Date: 2019-04-19
XI AN JIAOTONG UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the current use of non-viral vectors with high autoimmunity and potential carcinogenicity in the process of gene therapy, as well as the disadvantages of traditional fluorescent molecules such as poor hydrophilicity, photostability and stability in the body, the present invention The purpose is to provide a nano-gene carrier for in vivo targeted tumor imaging and treatment and its preparation method and application. The method is simple in process and the prepared material has good in vivo targeted tumor imaging and treatment capabilities

Method used

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  • Nano gene carrier for in-vivo targeting tumor imaging and treatment and preparation method and application thereof
  • Nano gene carrier for in-vivo targeting tumor imaging and treatment and preparation method and application thereof
  • Nano gene carrier for in-vivo targeting tumor imaging and treatment and preparation method and application thereof

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preparation example Construction

[0034] The present invention is committed to preparing a biocompatibility and good gene transfection, tumor targeting and fluorescence capabilities, so that it can be used for in vivo targeted tumor imaging and treatment. The preparation method comprises the following steps:

[0035] 1) Preparation of PPF polymer: PP prepolymer and tumor active targeting receptor molecules with a molar ratio of 1: (0.2-1.0) were mixed in MES buffer (pH 4-6), with EDC and NHS as catalysts The reaction is carried out; the reaction system uses NaOH solution to adjust the pH value of the solution to 8.0-9.0, and then stirs the reaction at room temperature. A PPF polymer is obtained. The active tumor targeting receptor molecule is folic acid. The structural formula is as follows:

[0036]

[0037]2) Preparation of PPR polymer: react the PP prepolymer and cell fluorescent molecules with a molar ratio of 1:(0.2~1.0) in MES buffer (pH 4-6), using EDC and NHS as catalysts; The system uses NaOH s...

Embodiment 1

[0045] 1) Preparation of PPF 0.2 polymer: Dissolve 0.2mmol folic acid completely in 50mM, pH 4-6 MES buffer, add 3mmol EDC and stir at room temperature for 30min, add 3mmol NHS and stir at room temperature for 12h, then add 1mmol PP prepolymer, and use NaOH solution was used to adjust the pH value of the solution to 8.0, and stirred at room temperature for 24 hours. After the final product was purified by dialysis, it was freeze-dried to obtain a PPF 0.2 polymer;

[0046] 2) PPR 0.2 polymer preparation: Dissolve 0.2mmol Rhodamine B completely in 50mM, pH 4-6 MES buffer, add 3mmol EDC and stir at room temperature for 30min, add 3mmol NHS and stir at room temperature for 12h, then add 1mmol PP prepolymer, And use NaOH solution to adjust the pH value of the solution to 9.0, and stir at room temperature for 24 hours. After the final product is purified by dialysis, it is freeze-dried to obtain the PPR 0.2 polymer;

[0047] 3) Preparation of nano-gene carrier: the above synthesized...

Embodiment 2

[0050] 1) Preparation of PPF 0.2 polymer: Dissolve 0.2mmol folic acid completely in 50mM, pH 4-6 MES buffer, add 3mmol EDC and stir at room temperature for 30min, add 3mmol NHS and stir at room temperature for 12h, then add 1mmol PP prepolymer, and use NaOH solution was used to adjust the pH value of the solution to 9.0, and stirred at room temperature for 24 hours. After the final product was purified by dialysis, it was freeze-dried to obtain a PPF 0.2 polymer;

[0051] 2) PPR 0.2 polymer preparation: Dissolve 0.2mmol Rhodamine B completely in 50mM, pH 4-6 MES buffer, add 3mmol EDC and stir at room temperature for 30min, add 3mmol NHS and stir at room temperature for 12h, then add 1mmol PP prepolymer, And use NaOH solution to adjust the pH value of the solution to 8.0, and stir at room temperature for 24 hours. After the final product is purified by dialysis, it is freeze-dried to obtain the PPR 0.2 polymer;

[0052] 3) Preparation of nano-gene carrier: the above synthesized...

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Abstract

The invention discloses a nano gene carrier for in-vivo targeting tumor imaging and treatment and a preparation method and application thereof. The method comprises the steps that 1, a PPF polymer isprepared; 2, a PPR polymer is prepared; 3, a nano gene carrier is prepared; 4, a PPFR polymer and a gene are added in an HEPES buffer solution, and then incubating in conducted in a water bath to formstable nanocomposites. by making certain tumor active targeting acceptor molecules or cell fluorescent molecule grafted to the PP polymer, and polymers are obtained. The obtained polymers have very good biocompatibility and good hydrophilicity, and a synthesis method of a thermal polymerization reaction and a catalytic reaction is environmentally friendly, easy to operate and low in cost of raw materials. Due to introduction of the certain tumor active targeting receptor molecules and cell fluorescent molecules, the targeting ability and biological imaging ability of the polymers for tumors are improved, and application of the polymers in biological imaging and the tumors is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of degradable biomedical materials, and specifically relates to a nano-gene carrier used for in vivo targeted tumor imaging and treatment, a preparation method and application thereof. Background technique [0002] With the continuous deepening of gene therapy research, the key to the success of gene therapy is to develop vectors with targeted, controllable and effective expression of the target gene. However, in the current gene therapy process, the widely used non-viral vectors have many safety problems in their clinical use due to their high immunogenicity and potential carcinogenicity. The unique advantages have attracted more and more attention. In addition, with the rapid development of various science and technology, intracellular fine structures, intercellular interactions, cell signal transduction, and the role of macromolecular proteins can be observed through fluorescence microscopy, but the auto...

Claims

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Application Information

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IPC IPC(8): A61K47/34A61K47/22A61K48/00A61K31/713A61K31/7088A61K49/00A61P35/00
CPCA61K31/7088A61K31/713A61K47/22A61K47/34A61K48/005A61K49/0041A61K49/0054A61P35/00
Inventor 雷波王敏
Owner XI AN JIAOTONG UNIV
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