Method and kit for simultaneous determination of 35 psychotropic drugs by HPLC-MS

Abstract

The invention discloses a method and kit for simultaneous determination of 35 kinds of psychotropic drugs by high performance liquid mass spectrometry. The simultaneous processing of 35 kinds of psychotropic drugs is realized by optimizing the chromatographic conditions and mass spectrometry conditions, and the pretreatment steps of psychotropic drugs are simple. Methanol is used for protein precipitation. Compared with the existing technology, the kit and the corresponding detection method greatly reduce the cost, greatly shorten the analysis time, improve the sensitivity and throughput, and meet the needs of clinical detection.

Description

technical field [0001] The invention relates to the field of psychotropic drug detection, in particular to a method and a kit for simultaneous determination of 35 psychotropic drugs by high performance liquid mass spectrometry. Background technique [0002] So far, more than 200 kinds of psychotropic drugs have been developed and used clinically. These psychotropic drugs have produced huge medical value and economic benefits. However, due to the problem of metabolic differences among individual patients, the therapeutic effect of these drugs on many patients is not satisfactory. Unsatisfactory, therefore, therapeutic drug monitoring (TDM) is receiving more and more attention. TDM is to titrate the dose of individual patients by quantitatively measuring the concentration of drugs in plasma or serum in order to obtain better curative effect and better tolerance. Tolerance, while also reducing the risk of poisoning. At present, among the chemical detection methods of TDM, LC-M...

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Problems solved by technology

[0005] First, the cost is high. At present, most LC-MS / MS methods use internal standards such as deuterium to correct matrix effects and other effects. The high cost of such internal standards will directly lead to high costs for the entire detection process;

[0006] Second, the throughput is low. Existing methods mostly measure one or several drugs at the same time, but rarely involve dozens of drugs, which affects the efficiency of detection;

[0007] Third, the analysis time is long. The analysis time of the LC part accounts for the vast majority of the measurement methods in the prior art, resulting in a long time for drug detection, and the general analysis time is longer than 5 minutes;

[0008] Fourth, the sensitivity is low. In the process of detecting multiple drugs at the same time, there is a problem of relatively low sensitivity of multiple targets, which affects the accuracy of detection.

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