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Active biological substance purification and separation medium

A technology for purification and separation of biological substances, applied in the fields of alkali metal oxides/hydroxides, inorganic chemistry, alkali metal compounds, etc., to achieve the effect of large adsorption capacity, large specific surface area and excellent performance

Inactive Publication Date: 2019-04-30
CHINA BLUESTAR CHENGRAND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problems of the existing purification and separation media, and provides a purification and separation medium for active biological substances, which has the specificity and advantages of the two purification and separation media

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] A matrix resin of an active biological substance purification and separation medium, comprising the following raw materials in parts by weight:

[0060] Oil phase:

[0061] Glycidyl methacrylate 60 parts

[0062] 30 parts of ethylene glycol dimethacrylate

[0063] 3 parts of divinylbenzene

[0064] Diethylbenzene 50 parts

[0065] 20 parts of 2-ethylhexanol

[0066] Quinol 20 servings

[0067] 3 parts dibenzoyl peroxide

[0068] water box:

[0069] 1500 parts deionized water

[0070] 5 parts of 10% polyvinyl alcohol in water

[0071] Biochemical grade gelatin 45 servings

[0072] 75 parts sodium chloride

[0073] 15 parts of 0.1% methylene blue aqueous solution.

Embodiment 2

[0075] A matrix resin of an active biological substance purification and separation medium, comprising the following raw materials in parts by weight:

[0076] Oil phase:

[0077] Glycidyl methacrylate 70 parts

[0078] 40 parts of ethylene glycol dimethacrylate

[0079] 5 parts of divinylbenzene

[0080] Diethylbenzene 60 parts

[0081] 2-Ethylhexanol 30 parts

[0082] Quinol 30 servings

[0083] 5 parts dibenzoyl peroxide

[0084] water box:

[0085] Deionized water 2500 parts

[0086] 10% polyvinyl alcohol in water 10 parts

[0087] Biochemical grade gelatin 75 servings

[0088] 125 parts sodium chloride

[0089] 25 parts of 0.1% methylene blue aqueous solution.

Embodiment 3

[0091] A matrix resin of an active biological substance purification and separation medium, comprising the following raw materials in parts by weight:

[0092] Oil phase:

[0093] Glycidyl methacrylate 65 parts

[0094] 35 parts of ethylene glycol dimethacrylate

[0095] 4 parts of divinylbenzene

[0096] 55 parts of diethylbenzene

[0097] 25 parts of 2-ethylhexanol

[0098] Quinol 25 servings

[0099] 4 parts dibenzoyl peroxide

[0100] water box:

[0101] Deionized water 2000 parts

[0102] 7.5 parts of 10% polyvinyl alcohol in water

[0103] Biochemical grade gelatin 65 servings

[0104] 100 parts sodium chloride

[0105] 20 parts of 0.1% methylene blue aqueous solution.

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PUM

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Abstract

The invention relates to an active biological substance purification and separation medium, and belongs to the technical field of purification and separation mediums, wherein the active biological substance purification and separation medium comprises 100-120 parts of a matrix resin, 500-1000 parts of diethylamine hydrochloride, 50-100 parts of alcohol, and 3-5 parts of sodium hydroxide. Accordingto the present invention, the prepared molecular separation and purification medium of the bioactive macromolecule integrates the advantages of natural mediums and synthetic separation mediums, and is a protein separation and purification medium with characteristics of macroporous structure, high adsorption rate, large specific surface area and large adsorption amount so as to provide a class ofseparation medium with excellent performance in the protein separation and purification.

Description

technical field [0001] The present invention relates to a purification and separation medium, more particularly, the present invention relates to a purification and separation medium of active biological substances, which belongs to the technical field of purification and separation media. Background technique [0002] With the continuous progress of bioengineering technology and the rapid increase in the demand for returning to nature, the demand for bioactive products in the fields of health food and biomedicine is increasing, and the requirements for separation and purification technology of bioactive products are also getting higher and higher. [0003] As the core component of separation and purification technology, the performance requirements of separation and purification media are becoming more and more demanding. Separation and purification media are generally divided into two categories: one is the natural carbohydrate separation media, which is mainly characteriz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F291/08C08F220/32C08F222/14C08F212/36B01J20/26B01J20/28
CPCB01J20/046C08F261/04C08F289/00B01J20/261B01J20/28011B01J20/28057B01J20/28085C08F222/102C08F220/325
Inventor 高川赵奕王煦怡刘咏梅
Owner CHINA BLUESTAR CHENGRAND CO LTD