Construction of photo-induced electrochemical sensor for alpha fetoprotein

A photoelectrochemical and alpha-fetoprotein technology, which is applied in the fields of nanomaterials and immunity, can solve the problems of being susceptible to external interference, long analysis time and high price, and achieve the effects of improving photoelectric conversion efficiency, simple operation and high sensitivity.

Inactive Publication Date: 2019-05-03
UNIV OF JINAN
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have inherent defects, such as the loss of some labeled antibodies, radiation hazards and environmental pollution, etc.; the markers required in fluo

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Construction of a photoelectrochemical sensor for alpha-fetoprotein, taking the detection of alpha-fetoprotein in serum as an example:

[0022] (1) CdS / SnS 2 / TiO 2 / The specific steps for the preparation of conductive glass are as follows: 0.05 g tetrabutyl titanate and 3.0 g anhydrous oxalic acid were dissolved in 30 mL N,N-dimethylformamide, and 15 mL of the solution was transferred to a 25 mL polytetrafluoroethylene liner In a stainless steel reactor, place the conductive glass in the reactor, react at 170 °C for 10 h, cool down to room temperature naturally, wash the conductive glass with ethanol three times, dry it at 60 °C, and then calcinate at 400 °C for 5 h to obtain the conductive glass Mesoporous TiO grown on the surface 2 ; the TiO 2 / Conductive glass is placed in a 25 mL reactor containing 6.0 mmol / L of SnCl 4 and 20 mmol / L L-cysteine ​​in 15 mL solution, reacted at 180 °C for 6 h, cooled naturally to room temperature, cleaned the conductive glass wit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses construction of a photo-induced electrochemical sensor for alpha fetoprotein. A cascaded construction sensing interface is constructed by using three kinds of semiconductor materials of CdS, SnS2 and TiO2, so that the photoelectric conversion efficiency is improved. (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) and N-hydroxysuccinimide are covalently coupledwith an alpha-fetoprotein capture antibody and the alpha fetoprotein is identified specifically. With a sandwiched mode, an electron donor is provided for a photo-induced electrochemical reaction ina sodium ascorbyl phosphate solution by combining an alkaline phosphatase/alpha-fetoprotein secondary antibody/gold nanoparticle complex. With a three-electrode system, the photocurrent of the systemis measured by using the white light xenon lamp with the wavelength of 200nm to 2500nm as a light source, so that the high-selectivity high-sensitivity detection of the alpha-fetoprotein is realized.

Description

technical field [0001] The invention relates to the fields of nanometer material technology, immune technology and the like, and more specifically relates to the construction of a photoelectrochemical sensor suitable for alpha-fetoprotein. Background technique [0002] Alpha-fetoprotein is the most important biomarker for the diagnosis of primary liver cancer. The detection of serum alpha-fetoprotein level is of great significance for the clinical diagnosis and early screening of liver cancer. Primary liver cancer is one of the common malignant tumors, and its morbidity and mortality rank first in the world. At present, the determination of alpha-fetoprotein content in serum is mainly carried out by immunoassay in clinical testing, and routine immunoassays include enzyme-linked immunosorbent assay, radioimmunoassay and fluorescent immunoassay. However, these methods have inherent defects, such as the loss of some labeled antibodies, radiation hazards and environmental pollu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N27/26G01N27/30G01N27/327G01N33/574
Inventor 葛慎光李娜付翠萍于京华刘海云颜梅
Owner UNIV OF JINAN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap