Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Blattella germanica sodium-ion channel resistance mutant gene and protein, and amplification primer, identification primer and identification method

A technology of sodium ion channel and Blattella germanica, applied in botany equipment and methods, biochemical equipment and methods, genetic engineering, etc., to achieve the effect of simple experimental operation, less manpower and material resources, and intuitive detection results

Pending Publication Date: 2019-05-07
中国人民解放军东部战区疾病预防控制中心
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to reduce the impact and harm brought by German cockroaches to people's life and work, various chemical insecticides have been used to kill it for a long time. However, due to the frequent use and various abuse of insecticides, Germany The reproduction speed of the cockroach is fast, and over time, the German cockroach has developed varying degrees of resistance to various commonly used insecticides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Blattella germanica sodium-ion channel resistance mutant gene and protein, and amplification primer, identification primer and identification method
  • Blattella germanica sodium-ion channel resistance mutant gene and protein, and amplification primer, identification primer and identification method
  • Blattella germanica sodium-ion channel resistance mutant gene and protein, and amplification primer, identification primer and identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: German cockroach resistance gene mutation and identification

[0032] (1) Determination of the knockdown time of the German cockroach: get the prepared medicine (perchlorinated, 98% permethrin, 95% tetramethrin, D-trans permethrin, concentration 0.4mg / ml) 15ml, Dilute the volume to 30ml with medical alcohol, and place it in a newly taken paper cup; completely wet the clean gauze cut into 50cm×40cm, and dry the gauze indoors (the final drug concentration is 30mg / m 2 ). Take 11 German cockroaches as a group, use a petri dish to buckle them upside down on the dried gauze, observe and record the knockdown situation of German cockroaches every 2 minutes, and take out the knocked down German cockroaches in time, Placed in a 1.5ml EP tube. Each drug was tested in two groups, a total of 88 rats.

[0033] (2) The tested German cockroaches (88 cockroaches) were extracted using the DNA extraction kit provided by Japan TAKARA Company according to the operating inst...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a blattella germanica sodium-ion channel resistance mutant gene. The DNA sequences of the mutant gene are shown as SEQ ID NO.1. A protein, an amplification primer, an identification primer and an identification method are also disclosed. A pair of primers can be used to amplify the gene fragments of related resistance gene mutation of blattella germanica, and sequencing canbe performed; and blattella germanica resistance genes can be identified through a four-primer ARMS-PCR method. The mutant gene can be widely applied to the identification demands of blattella germanica resistance gene mutation and corresponding scientific research fields.

Description

technical field [0001] The invention relates to a sodium ion channel resistance mutant gene of the German cockroach, a protein, an amplification primer, an identification primer and an identification method, and belongs to the field of biotechnology. Background technique [0002] The German cockroach is a common global household hygiene pest that prefers moist, warm conditions, but is also resistant to dry conditions. With the traffic activities of human beings, they are widely distributed all over the world. Under suitable conditions, a female can reproduce about 4 generations a year. The strong reproductive capacity of the German cockroach enables it to quickly become the local dominant indoor population. As an omnivorous species, almost everything can become its proper food source, whether it is sugar, starch, meat, or textiles, spoiled items, food and drink residues, body fluids and hairs, etc. are german cockroaches food. In addition to damaging food, furniture, tex...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12N15/11C07K14/435C12Q1/6858
Inventor 谭伟龙吕瑞辰艾乐乐王刚王宗才杨露朱长强吕恒丁晨曦
Owner 中国人民解放军东部战区疾病预防控制中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com