Thymalfasin peptide resin pyrolysis method

Abstract

The invention discloses a thymalfasin peptide resin pyrolysis method, and belongs to the field of polypeptide solid-phase synthesis. The method comprises the steps of taking thymalfasin peptide resin,adding a lysate which is 6-12 times the volume of the resin, conducting pyrolysis at 10-30 DEG C for 1-3 h, and filtering out resin to obtain a filtrate; adding the filtrate into ether which accountsfor 6 times or more of the volume of the filtrate; adding a 0.5M-1.5M sodium bicaronate or ammonium hydroxide solution which is 0.5-1 time the volume of the solution into the solution; adding an alkaline solution until the solution is clarified; conducting standing and layering, taking an upper-layer organic phase, adding a moderate amount of water for washing, merging the upper-layer organic phase with a lower-layer aqueous phase, and conducting vacuum pressure reduction to remove residual ether; adding acid to adjust the pH value to 2-8, and conducting filtering and purification. By means of the method, there is no need to use ether to wash crude peptide, the use amount of the ether is greatly reduced, the centrifuging operation is omitted at the same time, and the fire blast risk of the ether is lowered; the crude peptide drying step is omitted, the production labor hour is shortened, the risk of thymalfasin degradation is also lowered, and the purity of crude products is improved.

Description

Technical field: [0001] The invention relates to the field of solid-phase synthesis of polypeptides, in particular to a thymus method new peptide resin cracking method. Background technique: [0002] Thymus Faxin formerly known as thymosin α1, also known as thymus 28 peptide, thymosin α1 or Tα1. Thymus fasin is a kind of thymosin, a natural polypeptide produced by human thymus and present in the thymus circulatory system in the human body. Thymus fasin can promote the transformation of lymphocytes, enhance the phagocytic activity of macrophages, and can be used to treat various immunodeficiency diseases. [0003] In the treatment of chronic hepatitis B with thymus fasin alone, domestic and foreign studies have shown that the sustained response rate is about 37%, which is similar to that of interferon alone, and there is no toxic side effect of interferon. The clinical research on the application of thymusfasin in the treatment of chronic hepatitis B in Taiwan, China shows ...

AI Technical Summary

Problems solved by technology

Ether solutions such as ether MTBE have potential safety hazards of explosion;

[0014] 2. Vacuum drying takes a long time, usually more than 8 hours;

[0015] 3. Due to the long time of the crude peptide state, the product is degraded and a large Asp is produced. 28 - Thymofasin

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