A kind of preparation method of reduced graphene oxide-polydopamine-glutathione composite material
A polydopamine and glutathione technology, applied in the field of composite materials, can solve problems such as narrow application
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Embodiment 1
[0021] (1) Preparation of graphite oxide: the first step of pre-oxidation process: 1.25gK 2 S 2 o 8 and 1.25gP 2 o 5 Add to 12.5mL concentrated H at one time 2 SO 4 Then add 1g of graphite powder, heat the mixed solution to 80°C, and heat to reflux for 5h under magnetic stirring. After cooling, add 200mL water to the solution to dilute, then filter, wash neutral, and vacuum at 60°C Set aside to dry. The second step is further oxidation process: Weigh 1g of the above-mentioned dried graphite oxide, and disperse it in 120mL of mixed acid of concentrated sulfuric acid and phosphoric acid (the volume ratio of concentrated sulfuric acid and phosphoric acid is 3:1) under ice bath conditions, and keep the temperature at 0 ~5°C and 9g of potassium permanganate was added under constant stirring, then the temperature was raised to 50°C and stirred for 12h. Cool the system temperature to room temperature, add 200mL of ice water and 5mL of 30% hydrogen peroxide respectively, and ke...
Embodiment 2
[0027] (1) Preparation of graphite oxide: same as Example 1;
[0028] (2) Preparation of reduced graphene oxide-polydopamine: Disperse 100mg of graphite oxide in 50mL of deionized water (concentration 2mg / ml), and sonicate to form a uniform suspension; then dissolve 50mg of dopamine hydrochloride in 150mL of Tris-HCl (pH=8.5) buffer solution, then add it to the suspension of graphite oxide, stir vigorously at 60°C~65°C for 24h, let it stand for 30~40min, separate, wash with water, and dry to obtain reduced graphite oxide ene-polydopamine composites;
[0029](3) Preparation of reduced graphene oxide-polydopamine-glutathione: Dissolve 30 mg of reduced graphene oxide-polydopamine in 30 mL of Tris-Hcl (pH=8.5) buffer solution, and sonicate for 5-6 hours ;Add 60mg of glutathione to the reduced graphene oxide-polydopamine solution, then add a small amount (13.5mg) of manganese dioxide, and stir and react at 40°C~50°C for 24 hours; after the reaction is completed, filter and freeze ...
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